CAZyme Information: MGYG000001995_02608

Basic Information

• Species: Parabacteroides sp900541965
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; Parabacteroides sp900541965
• CAZyme ID: MGYG000001995_02608
• CAZy Family: GH63
• CAZyme Description: Glucosidase YgjK

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
639	MGYG000001995_38|CGC1	71619.05	5.0747

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001995	4648473	MAG	Spain	Europe

• Gene Location: Start: 42608; End: 44527 Strand: +

Full Sequence      

MNKKFSVITFTLLALFGWAGCTVQQSLPVYTAEKYGNIVDIRYTPDTTSRCRGWFTDAGS
WFGFTLPQENKWVNGFCGPFHLDMNRREWLSDALVKVGFADEDTAVAYTPDSVSYFPGRL
YMASSSGKGRIEQELVFIDKGTALLSCRSDRDALFRFSGKTLPGGTTSLVDKNTLTLTLP
DGEGVALTFSPEMGILSTENGYETLSLKPGKAAVVISFFEKDSLSLPVLQKAASIQTDPE
KYKAETAARWEGYLTKVLRPDMPEAYNRIAVKAVVTLMANWKSARGDLFHDGVVPSHAVG
YFMGFWGWDSWKHAAGIARFAPELAKDQVRTMFDYQTPEGMIIDCIYSDAAENNARDSKP
PLAAWAVDEIYKATGDSSFVKEMFPKLLAYYKWWFKYRDHNQNGICEFGSCDGTLEAAAW
ESGMDNAIRFDDAVMVKNGENAWSMNQESVDLNAFLALEHRLLTKLAPVAGATFDEPSRS
ADVQNYFFVPERGFFFDKRLDGTFVLEEGTEACIPLWAGIATKEQAADCMKLFTDTAKFA
TYIPFPTIAADNPKFMPAGYWRGPIWLDQVYFGISGIRNYGYKKEADAFTEQVFNRLQGL
KEDAPIHENYDTHNGGRLKAPHFSWSSAHLLMLYEEYGK

Enzyme Prediction     

No EC number prediction in MGYG000001995_02608.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH63	230	637	1.6e-105	0.5

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10137	PRK10137	2.58e-128	1	637	1	783	alpha-glucosidase; Provisional
COG3408	GDB1	6.29e-16	291	634	270	603	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
pfam01204	Trehalase	6.60e-11	293	638	119	509	Trehalase. Trehalase (EC:3.2.1.28) is known to recycle trehalose to glucose. Trehalose is a physiological hallmark of heat-shock response in yeast and protects of proteins and membranes against a variety of stresses. This family is found in conjunction with pfam07492 in fungi.
PLN02567	PLN02567	4.27e-05	353	638	193	545	alpha,alpha-trehalase
pfam03200	Glyco_hydro_63	0.001	377	635	185	494	Glycosyl hydrolase family 63 C-terminal domain. This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyze the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the C-terminal catalytic domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT86878.1	1.89e-281	1	639	1	644
QJR62245.1	5.39e-281	1	639	1	644
QJR74523.1	5.39e-281	1	639	1	644
QJR66502.1	5.39e-281	1	639	1	644
AND18792.1	1.08e-280	1	639	1	644

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W7S_A	5.28e-75	32	637	2	756	Escherichiacoli K12 YgjK complexed with glucose [Escherichia coli K-12],3W7S_B Escherichia coli K12 YgjK complexed with glucose [Escherichia coli K-12],3W7T_A Escherichia coli K12 YgjK complexed with mannose [Escherichia coli K-12],3W7T_B Escherichia coli K12 YgjK complexed with mannose [Escherichia coli K-12],3W7U_A Escherichia coli K12 YgjK complexed with galactose [Escherichia coli K-12],3W7U_B Escherichia coli K12 YgjK complexed with galactose [Escherichia coli K-12]
3W7X_A	2.72e-74	32	637	2	756	Crystalstructure of E. coli YgjK D324N complexed with melibiose [Escherichia coli K-12],3W7X_B Crystal structure of E. coli YgjK D324N complexed with melibiose [Escherichia coli K-12],5CA3_A Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12],5CA3_B Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12]
3W7W_A	3.77e-74	32	637	2	756	Crystalstructure of E. coli YgjK E727A complexed with 2-O-alpha-D-glucopyranosyl-alpha-D-galactopyranose [Escherichia coli K-12],3W7W_B Crystal structure of E. coli YgjK E727A complexed with 2-O-alpha-D-glucopyranosyl-alpha-D-galactopyranose [Escherichia coli K-12],5GW7_A Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12],5GW7_B Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12]
3D3I_A	5.04e-71	32	637	3	757	Crystalstructural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63 [Escherichia coli K-12],3D3I_B Crystal structural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63 [Escherichia coli K-12]
6XUX_A	3.64e-37	17	410	313	768	ChainA, Nanobody,Glucosidase YgjK,Glucosidase YgjK,Nanobody [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P42592	3.10e-74	23	637	16	779	Glucosidase YgjK OS=Escherichia coli (strain K12) OX=83333 GN=ygjK PE=1 SV=1
D8QTR2	5.61e-18	298	628	90	479	Mannosylglycerate hydrolase MGH1 OS=Selaginella moellendorffii OX=88036 GN=MGH PE=1 SV=1
D8T3S4	1.32e-17	298	628	90	479	Mannosylglycerate hydrolase MGH2 OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_447962 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000195	0.031575	0.968163	0.000033	0.000042	0.000029

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001995_02608.