CAZyme Information: MGYG000001994_01128

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Schaedlerella
• CAZyme ID: MGYG000001994_01128
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1195		131573.43	4.5408

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001994	2695341	MAG	Spain	Europe

• Gene Location: Start: 31411; End: 34998 Strand: +

Full Sequence      

MKTRFKKFVGISMATVMSVSLLSTTALAEQPAAEPTPLANDNLLRLWYDEPANQNDYYAW
ETEALAIGNGYMGGLLYGGVQKDKIHINEKTVWKGGPADSYNYKYGNTNPTETDEDLQTI
KDNLTAIREKLDDKSEYVFGFSEDSYESSGTSTYGEPMNWLNKLMGDLTGYDAPQDFANL
YISNDAIDESKVENYVRDLDMRTALSTINYDYEGVHYTREYFNSHPDNILVVRLTADQAG
KITFKTNLSDCTGSGVLSTNEDTITMTSTLRRNNLNTEAQLKVLNDSGELTSDGTSITVN
GADSATLILACGTDYKMELPSFRGEHPHEAITSRIASASNIISEEGYDVLKKRHVDDHSA
LFSRMELGFNEEIPQIPTDELIKKYRNMVDNTGGEIPTEAEQRALEIMCYQFGRYLTIAG
SREGSLPTNLQGVWGEGGFAWGGDYHFNINIQMNYWPTMASNLGECHIPYNDYLAVLREA
GRGAAAAAFGIKSNEGEENGWLVGCFSTPYMFATMGQKNNAAGWNPIGSAWALLNCYEYY
LYTGDQEYLRNELYPSMKEVANFWNEALYWSEYQQRYVSSPSYSPENGPIVSGASYDQQF
IWQHFENTIQAARTLGVDEDLITVWEDKQSKLDPVIVGDDGQVKEWFEETSFGKAQAGDL
EEIDIPQWRESLGAQSGGQQPPHRHLSHLMALYPGNLINKDNAEFMNAAMVSLNERGLDA
TGWSKAHKLNLWARTGHSDEAFQIVQSAVGGGNSGFLTNMFSSHGGGANYKQRPIFQIDG
NYGYTAGVNEMLLQSQLGYVQFLPALPEQWNTGYVNGIVARGNFVVDMKWTDGVAESFKI
TSRNGGDFIGEYPNLSEYVVKDSAGNFVATSVPSDDVNKLSFPTTAGESYTISKATTYNI
SIAETENGTVSVPTTANEGSIVALSASPEKGYILNMITVTDASGNLVETTAQEDGSYTFV
MPSSDVTVTAAFKEDTPVEVSYAIHVPEMKNGTIEIPETAVENSTVQLIVKPVKGYVVAN
VVIHTLDGQAVSIADQGNGVYTFTMPASDITVNATFRPESGTETTYKVTIQDSKNGKVTV
PQTSYTVGSKVILTAVPDKGYKLDKITAVDSKNQTIKLKKQKDGTYTFEMPANDVTIKAS
FKTTGKPAVPDSKPNKPGTPPKTGDNSHLTLWSSLVLLSLGSVITFTFYRKRKHK

Enzyme Prediction     

No EC number prediction in MGYG000001994_01128.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	42	848	1.6e-234	0.9750692520775623

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	3.69e-50	46	317	1	233	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
pfam18998	Flg_new_2	2.00e-09	898	976	1	74	Divergent InlB B-repeat domain. This family of domains are found in bacterial cell surface proteins. They are often found in tandem array. This domain is closely related to pfam09479.
pfam18998	Flg_new_2	1.84e-08	1066	1142	1	71	Divergent InlB B-repeat domain. This family of domains are found in bacterial cell surface proteins. They are often found in tandem array. This domain is closely related to pfam09479.
pfam03632	Glyco_hydro_65m	0.001	530	675	122	264	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL25699.1	0.0	1	892	1	888
AMP52821.1	7.86e-272	435	892	1	456
QGH70015.1	1.38e-254	5	932	2	905
AZM50986.1	3.72e-244	28	892	16	845
AMN36379.1	6.03e-190	1	892	1	811

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2EAB_A	3.86e-148	46	894	20	896	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAD_A	2.88e-147	46	894	20	896	ChainA, Alpha-fucosidase [Bifidobacterium bifidum],2EAD_B Chain B, Alpha-fucosidase [Bifidobacterium bifidum]
2EAE_A	5.48e-147	46	894	19	895	ChainA, Alpha-fucosidase [Bifidobacterium bifidum]
2RDY_A	2.67e-104	44	842	3	744	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]
4UFC_A	1.88e-99	44	845	22	736	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	2.33e-84	62	843	64	805	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	1.91e-75	46	847	23	775	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1
Q2USL3	8.72e-57	46	841	18	706	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2
Q5AU81	1.21e-54	67	846	46	792	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000255	0.998985	0.000170	0.000225	0.000176	0.000147

TMHMM  Annotations     

start	end
1170	1189