CAZyme Information: MGYG000001977_03287

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides
• CAZyme ID: MGYG000001977_03287
• CAZy Family: GH18
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
577	MGYG000001977_215|CGC1	65512.54	6.456

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001977	4322077	MAG	Denmark	Europe

• Gene Location: Start: 3594; End: 5327 Strand: +

Full Sequence      

MKNFRLIVLSVLAVLLSCGNLKAADESKIPLDSKVVVAYVTSWSHVIPDPKYMTHINYAF
GHVNEAFDGVSIANEGRLRQMVELKKQKPDLNVLLSIGGWGSGRFSEMAANEKYRRAFAK
DCRWVLKEYGLDGIDIDWEYPTSNAAGISSSPDDTKNFTLLMRDIRKAIGPKKLLTLATV
ASAEYIDFPAILPYINFVNIMSYDMGNAPKHHSALYSSDNSGWMTGDKAVKAHLAAGVPA
DKLVMGMPFYGRGGKEYPNFQDFNKVGYAKGFRECWDETAQVPYLVNEKDTLVFGYENPR
SLAIKCQYILKQNLLGGMYWDYDGDNEQGDLRRTVYENLIERKPFHDRSYRVLVLTESAG
QHKPFSDAALKWLVDESKVQNLQIQVLNNTRLLAQKEVLDGTDLVIQLDFPPYTWPKEAE
QNFINYINEGRGGWIGFHHATLLGEFDGYPMWQWFSDFMGGIRFQSYIAALADGTVVVED
KEHPVMKNVDASFVLRDDEWYTYDKSPRPNVRVLAHVDESTYSPVSDVKMGDHPVLWVNE
KVKARNVYFQMGHSPKLFKSDDFTTMLHNAISWTLGK

Enzyme Prediction     

No EC number prediction in MGYG000001977_03287.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	34	332	1.8e-72	0.9594594594594594

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06548	GH18_chitinase	3.10e-106	36	325	1	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
smart00636	Glyco_18	6.67e-81	35	325	1	334	Glyco_18 domain.
pfam00704	Glyco_hydro_18	6.81e-78	35	325	1	307	Glycosyl hydrolases family 18.
cd02872	GH18_chitolectin_chitotriosidase	3.70e-70	36	325	1	341	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.
COG3325	ChiA	9.00e-61	7	339	7	436	Chitinase, GH18 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT34047.1	0.0	1	577	1	577
ADV45176.1	0.0	1	577	1	577
QDO71383.1	0.0	1	577	1	576
QUT90190.1	4.23e-313	1	577	1	573
ALJ58703.1	6.97e-312	1	577	1	573

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6INX_A	3.87e-58	51	339	25	342	Structuralinsights into a novel glycoside hydrolase family 18 N-acetylglucosaminidase from Paenibacillus barengoltzii [Paenibacillus barengoltzii]
3QOK_A	2.02e-52	49	339	50	416	ChainA, Putative chitinase II [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
6TSB_AAA	1.09e-51	25	335	27	346	ChainAAA, Peroxiredoxin [Clostridioides difficile 630]
6T9M_AAA	1.75e-51	25	335	46	365	ChainAAA, Peroxiredoxin [Clostridioides difficile 630]
4NZC_A	8.54e-49	49	339	30	396	Crystalstructure of Chitinase D from Serratia proteamaculans at 1.45 Angstrom resolution [Serratia proteamaculans 568],4Q22_A Crystal structure of Chitinase D from Serratia proteamaculans in complex with N-acetyl glucosamine at 1.93 Angstrom resolution [Serratia proteamaculans 568]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20533	6.54e-42	32	328	42	441	Chitinase A1 OS=Niallia circulans OX=1397 GN=chiA1 PE=1 SV=1
Q9W092	1.80e-32	30	338	37	404	Probable chitinase 2 OS=Drosophila melanogaster OX=7227 GN=Cht2 PE=1 SV=1
Q11174	3.74e-31	30	326	49	400	Probable endochitinase OS=Caenorhabditis elegans OX=6239 GN=cht-1 PE=1 SV=1
Q13231	4.16e-31	36	329	24	367	Chitotriosidase-1 OS=Homo sapiens OX=9606 GN=CHIT1 PE=1 SV=1
Q95M17	4.48e-31	36	329	24	369	Acidic mammalian chitinase OS=Bos taurus OX=9913 GN=CHIA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000411	0.486344	0.512626	0.000216	0.000216	0.000180

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001977_03287.