CAZyme Information: MGYG000001925_01029

Basic Information

• Species: Phocaeicola sp900544675
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola sp900544675
• CAZyme ID: MGYG000001925_01029
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
782	MGYG000001925_10|CGC2	86698.72	5.1557

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001925	3441241	MAG	Denmark	Europe

• Gene Location: Start: 42389; End: 44737 Strand: -

Full Sequence      

MKNLKNIVLASVLCTGACLSAQGQTVQPAIPSDPQIEANIQEWLKKMTLEQKIGQMCEIT
IDVVTDFKASQKNGFTLDKGKLDTVIGKYKVGSLLNVPLSVAQTKEKWAEAIRQIQELSM
KEIGIPCIYGVDQIHGTTYTLGGTMFPQGINMGASFNRDLVKREAEISAYETKAGCIPWT
YAPVVDLGRDPRWSRMWENYGEDAYVNAEMGKASVAGFQGEDPNHIGPDHVAACMKHYMG
YGVPVSGKDRTPSSISRSDMREKHFAPFLAAVRNGALSVMVNSGVDNGMPFHANKEFLTQ
WLKEDLNWDGLVVTDWADINNLCTRDHIAATKKEAIKIAINAGIDMSMVPYEVSFCDYLK
ELVEEGEVPMSRIDDAVARVLRLKYRLGLFDKPYWSTEDYPKFGSEEFAKVALQAAEESE
VLLKNTDNILPLAKGKKILLAGPNANSMRCLNGGWSYSWQGHRADECAQAYNTIYEALCN
KFGKENIIYEPGVTYAPYKNDNWWEENTPEIEKSVAAAQNADVIIACIGENSYCETPGNL
TDLNLSMNQQNLVKALAATGKPVILILNEGRPRLIKDIEPLAKAVVNIMLPGNYGGDALA
NLLAGDANFSGKMPYTYSKHINALATYDYKPCENIGQMGGNYNYDSVMDIQWPFGFGLSY
TTYKYSNLKVDKPAFDADDELTFTVDVTNTGKVAGKESVLLFSKDLVASSTPDNIRLRNF
TKIALNPGETKTVTLKIKGSDLAFVNYYGQWTLEKGDFKVKCGDQWIDLQCTQTKVWDTP
NK

Enzyme Prediction     

EC	3.2.1.21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	121	349	1.7e-64	0.9768518518518519

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	2.95e-150	1	763	1	751	beta-glucosidase BglX.
COG1472	BglX	9.04e-81	47	465	1	386	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	3.31e-73	48	383	1	316	Glycosyl hydrolase family 3 N terminal domain.
PLN03080	PLN03080	6.52e-56	114	764	65	770	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	5.96e-50	421	660	2	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADY35485.1	0.0	1	782	1	782
ADY36471.1	0.0	1	782	1	782
QRM99675.1	0.0	1	782	1	781
QUT79256.1	0.0	1	782	1	781
QDM10337.1	0.0	1	782	1	781

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JP0_A	0.0	24	782	1	759	Bacteroidesovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus],5JP0_B Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus]
5XXL_A	6.07e-112	44	770	18	745	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	3.27e-111	44	770	18	745	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
5TF0_A	4.07e-109	45	776	18	750	CrystalStructure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393],5TF0_B Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393]
6R5P_A	4.52e-98	16	763	18	751	ChainA, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5P_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXU3	0.0	1	782	1	781	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
Q23892	3.25e-147	44	769	84	818	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
Q56078	3.29e-107	37	763	35	751	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
P33363	1.76e-106	37	763	35	751	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
T2KMH0	8.69e-100	121	763	61	707	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000709	0.694044	0.304249	0.000377	0.000335	0.000276

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001925_01029.