CAZyme Information: MGYG000001855_00568

Basic Information

• Species: CAG-632 sp000431515
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-632; CAG-632 sp000431515
• CAZyme ID: MGYG000001855_00568
• CAZy Family: GT4
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
422	MGYG000001855_1|CGC4	49248.66	5.1071

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001855	2470907	MAG	Denmark	Europe

• Gene Location: Start: 612194; End: 613462 Strand: -

Full Sequence      

MSLSKKIIDLSEKMRPVLVKVVPMKLLRYMKDKVQERELKRMQNRRRKPYEPGHYEPGIN
LIGCIRAEIGLGQSCRLLADELEHTGISYMIRDFLLDGKLRAEEHSWDHKIEEQPRYGIN
LLHIEPLDLIMAYMKEEPELWDYRYNIAFWLWELEEFPQDWMKSLDLVDEIWTPSEFTSS
CIRKVTDKPVITIPYCVTAPTEPQYNRAYFKLPEDRFLFLVMYDTNSTMDRKNPVGALKA
FCQAFSPEDQSVGLVLKMNNPRKEDLDVLKDQLKEYSNIYYITEVMDKVVVNSLIREADV
FVSLHRAEGFGLVMAEAMLNGTPCIATDWSSNTEFMNSDVACMVDYTFTTLEKSSPPYEK
GARWADANIDTAAAYMKKLKENPEYYRRLAENGKAYIEEKLGMEQAVRAIKQRIGEIEHD
FT

Enzyme Prediction     

No EC number prediction in MGYG000001855_00568.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT4	231	360	3.2e-16	0.675

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0438	RfaB	1.82e-19	105	417	88	377	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
cd03801	GT4_PimA-like	3.43e-19	119	410	88	361	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
cd01635	Glycosyltransferase_GTB-type	5.43e-18	188	344	76	234	glycosyltransferase family 1 and related proteins with GTB topology. Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. The structures of the formed glycoconjugates are extremely diverse, reflecting a wide range of biological functions. The members of this family share a common GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
pfam00534	Glycos_transf_1	9.11e-14	231	394	14	156	Glycosyl transferases group 1. Mutations in this domain of PIGA lead to disease (Paroxysmal Nocturnal haemoglobinuria). Members of this family transfer activated sugars to a variety of substrates, including glycogen, Fructose-6-phosphate and lipopolysaccharides. Members of this family transfer UDP, ADP, GDP or CMP linked sugars. The eukaryotic glycogen synthases may be distant members of this family.
cd03809	GT4_MtfB-like	1.43e-13	128	395	110	346	glycosyltransferases MtfB, WbpX, and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. MtfB (mannosyltransferase B) in E. coli has been shown to direct the growth of the O9-specific polysaccharide chain. It transfers two mannoses into the position 3 of the previously synthesized polysaccharide.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACR71127.1	1.05e-126	1	417	1	426
QUA52503.1	1.96e-102	48	417	53	422
QWT52109.1	3.97e-102	42	403	12	376
AZS17521.1	4.75e-98	55	414	61	422
ASS69318.1	7.29e-98	58	414	42	400

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001855_00568.