CAZyme Information: MGYG000001748_00587

Basic Information

• Species: CAG-56 sp900762665
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56; CAG-56 sp900762665
• CAZyme ID: MGYG000001748_00587
• CAZy Family: GH127
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2282	MGYG000001748_4|CGC3	249705.31	5.1642

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001748	4218051	MAG	Sweden	Europe

• Gene Location: Start: 152241; End: 159089 Strand: +

Full Sequence      

MKKRVIAFVMCLVLVFSMLPAACLDSVAAAETEVEVELGENIALKATAYADYTNTGTNVK
NVNNGILATGSATTWNTWKKGGAEYPAPVWLEWDVDYMVSGMRIIWWADNANITGSEGVT
FPKSCEVEYLNDNGEWTKITGMQNEAGAQVDTMGVLYNPSDGNGLNGSNKLWNVVTFPEP
VRTKKLRLLIERNGTGDNGIGISEWEVFGGKAPSDLGVGENIALKGQVEAEYSNNGTSPS
NVNDGKLAVGASTTWNTWKLNGYTYPCPITIKWDKEYEISSMKLMWWADDIDGPGSSGGG
DGVMFPKSCQVQYYDLSQKAWIDAAGMVDETGAMVDALGVKYGTAAEAGEDQTAFTQEKN
RYWNGVIFKSKIKTNQLRLLIDRQDGATVNSGIGIGEWEVYGEEAVRNIADGVNIASRAV
ATAEYSNTDTAPERVNDLSLAGNAATSWNTWKLGGDLTYPQPITLTWDEPYDISSMRVMW
WADDLEAGTSSGDGVLYPKSCEAWYFDYDQNDWAQLTDMTNENGEKVSTVGVLGEGTQGN
NRVWNGVLFNTPVKTTKLRLYIDRPDGTTERGGIGIGEWEVYGEKVKNEMVSAKISGKNR
LVKSEVSSYYGVSVPGELSGTFDYEWSVPEESKDILQIIGGSDQQEAQVKALDTGKGTLH
LKLTYKEDDIQTVRETSFEVQIDEITSVDDYITTTMEGREPILPKTVVANGITFDTPTPS
LKSSTNPSFDFAETFDSKLVPVEWEPIDPSAYSKAGNVFEVTGKVLNSDLTATAKITVNK
KVAAPDENSTVTFENIKLTDEFWLPKQRVNALNSLNAAIYRIGLSSGGEPNFDNAIKRLN
GEPYSPFSGLVFQDTDIYKTLEAISYTLSVIQDETEPEITEQKKNLEDTLQRWIEKIEKV
QYADGYINTHFTLRAAAYEGGRAPGTHRWRNFNNHEMYNAGHFLESVVAYTRYREGIGDP
DYSLYVAGRRFADHVVERFGPEGTRHEVPGHEEIELALVKFAKLVEEYEGAGAGQKYVDT
AKLLIDRRGEDYKLRESGYRGYTEGVREYSQDAKPFTEETNAVGHAVRAAYLYTGATDVA
RLLPDSDPDKTAYMSILDNIWDSVANRKTYITGGIGVASHGEDFGGDYELPNNDSYNEIC
ASIALTNWNQRMNLVHEDTKYVDVMERALYNGVLVGTNLEGNKFYYATKLEIPKKGNVVD
GGMYGGVQRQDWFNCACCPPNLMRTIAKLSEYIYTTHKDNVFVNLFIGSDGHVNVDGTRV
GLKQETQYPWNGAVKLTVQPEEKKSFTMNIRIPGWVKEQKDKKVTIRVNDTEVTEKAKKG
YVAITREWDVNDVITIDMPMEIRKTEANPKVVTNKDKIAIERGPIVYAIEKAGNAQINKE
TISENEFDPRNFVIPRDARLTADYDADLLKGVMEITGDVTYKKGTETVPAKLQAIPYYAS
NNRGDSGVYGDGISTRMTVWTAASGTPVARYTVSLDPNGGTVSKTSFDVADGSEIGELPE
PERLYHLFQGWYTEKEGGEKVTEETIVTGNMTLYAHWVLNSEVAIRTVTLNPNGGKVTPA
SLDIESGNAIGALPTPTKTGSVFQGWFTSLEGGKQVQANDIVNENMTIFARWIDQDVVFG
ASGAQTETDGTYLIAMTGGTSSEGADHAAAYIQGVTATGGSPLGSTRIGCLAFKLLDEWK
AMDPDKITATVTMNVSRVNGNLGTAKTKAALFAVDKPLNEITLTNASTYPAKDLDYSATA
TVFSNEWIAANDTGNKTFDVTDMVKQALKDPDATHAIFRLQTVISGFYVTNQGENAPALI
VSEKPAEKEAGKLTIKCDGFTYNGKTKPNPQVVSTTNTGAAVTYKYYSDQACEKEITAPV
NAGTYYVKGTAAETETHTSAASAAVKFTIAKAQPTYTDTSEIKLTAKTGQTLNDVKLQLP
DGFHFEADLTTPVGEVGKNTHKVSFTPADTDNYEIVTGLKVIIMVTSPEKELGSLTISCA
GFVYTGTKKPVPMVVSTTNSDAAVTYRYYSDQACTKEIAAPVNAGTYYVIGIAAETETHM
TATSAAVSFTIGKAEGAITIQCVGFVSNGKTKPSPQIIAITGQAAKVSYKYYSDQACKKE
ISAPVSAGTYYVRATAEETQNYRSAVSNVVKFTIQREIPKKNSSWKVGNYNYKVTKAAAK
NGTVELKSPRKKTLKTATIPSTIKINGYTFKVTSISANAFKNNKRLRKVTIGSNVKKIGK
SAFSGCKSLRTIYLKTKVLTSVGKSALKGISSKAKIQSPKGKKKAYTKLFKNKGQKKTVK
VK

Enzyme Prediction     

No EC number prediction in MGYG000001748_00587.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	797	1368	7.9e-170	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	1.94e-128	844	1465	55	589	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	1.81e-127	795	1368	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
sd00036	LRR_3	2.02e-15	2174	2272	16	127	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
pfam09479	Flg_new	2.81e-14	1471	1536	1	65	Listeria-Bacteroides repeat domain (List_Bact_rpt). This model describes a conserved core region of about 43 residues, which occurs in at least two families of tandem repeats. These include 78-residue repeats which occur from 2 to 15 times in some proteins of Bacteroides forsythus ATCC 43037, and 70-residue repeats found in families of internalins of Listeria species. Single copies are found in proteins of Fibrobacter succinogenes, Geobacter sulfurreducens, and a few other bacteria.
sd00036	LRR_3	1.41e-12	2174	2240	62	142	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGS53277.1	3.79e-264	411	1465	29	1078
AKA69366.1	2.30e-144	791	1521	53	745
AWI04507.1	1.09e-143	791	1521	53	745
QGY44796.1	4.28e-128	789	1460	35	648
BBE18432.1	1.05e-127	791	1444	32	640

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	1.95e-121	795	1445	15	638	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.04e-117	795	1445	15	638	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
6EX6_A	4.36e-107	791	1445	10	616	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
3WRE_A	4.51e-75	836	1443	57	647	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	5.76e-75	836	1443	57	647	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	2.47e-74	836	1443	57	647	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000087	0.117582	0.882220	0.000049	0.000043	0.000035

TMHMM  Annotations     

start	end
5	22