CAZyme Information: MGYG000001712_02235

Basic Information

• Species: Bacillus_A thuringiensis_S
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae_G; Bacillus_A; Bacillus_A thuringiensis_S
• CAZyme ID: MGYG000001712_02235
• CAZy Family: GT2
• CAZyme Description: Dimodular nonribosomal peptide synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2385	MGYG000001712_1|CGC21	267564.05	4.8494

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001712	5736823	Isolate	United States	North America

• Gene Location: Start: 2195469; End: 2202626 Strand: +

Full Sequence      

MPNSQKIRHSLSSAQSGMWFAQQLDPLNPIYNTGEYVEINGNIHQEIFELAVRKVVIEAE
ALHVRFEEDEIGPWQVIEESQFYMHFIDVRKEENPEEAAKVWMKNDLSMPVDLKKDILFT
EALIQVENNRFFWYQRIHHIVMDGYGFSLLSQKVANEYTSLIEETNKNEKPFGSLTKVVQ
EDIEYRESKQFQEDRTFWLEKFADEPEVVSLAERAPRTSNGFSRETAYLSSSSTKTLLED
INISLTSWPEFIVAVTSIYMHKLTGENDIVLGLPMMGRLGSVSIHTPSMVMNLVPLRLTV
TPNITLAELLQQVSKEIRDVRRHHKYRHEELRRDLKLLGENQRLFGPLVNVMPFDYGLNF
AGNRGITHNLSAGPVDDLSINVYKRFDQNELMIHFDANPEVYNGAELALHKERFMNLFEL
VIKNYEKNESIGKINITLPEENHKVLLEWNETKEDDELISLPISFEKQVQKNPNKLAITC
DGINLTYKELNERANELAHYLVEEGIRPNQFVALVFPRSIEMVVSMLAVLKAGAAYLPID
PEYPAERVNYIVDDAKPVCIITHSSVSSKLVIENGMKKIVLDGEETKLALHTYSRMNIAC
KNDVLLLNPAYTIYTSGSTGNPKGVIVPMRGLSNFLMAMQQKFSLNENDHLLAVTTFAFD
ISALEIYLPLISGASLTIAQKEDIQEPSALTTLLQEERVTVMQATPTLWQALVTDYPEKL
QGLNILVGGEALPAHLANKLKELGCSITNLYGPTETTIWSTFMNIDESEKGIPPIGKPIS
NTEVYVLDAGLQPVPPGVIGELYIAGEGLASGYLGKPELTAERFVANPYGESGKRMYRTG
DLVKWRSDGALEYISRADHQIKIRGFRIELAEIETVLQRHENIQQAVVMVREDRPNDKRI
IAYIIAEEKEPINLSEIRSYVSESLANYMIPSAFVVLEELPLTPNGKVDRKKLPAPNFNG
MNNERVARNPKEEILCDLFAEVLGVSRISIDDNFFEMGGHSLLASRLMARIRETLSVELG
IGKLFESPTVAELAKQLNHAKSARPAIQKASRPNEVPLSFAQRRLWFLNCLEGPSPTYNI
PLVIRMNGILNEEALQGAFYDVVEKHETLRTIFPNVLGSSYQKILDMENLNLEMIITKTC
KDELESVLSEAVRYSFNLDIEPAVRLQLFTVSENEHVLLILLHHIVGDGWSLQPLTRDFT
AAYKARCQGDRVQLETLPVQYADYALWQQQLLGDETTPESLISTQLDFWKEELKGLPDQM
ELPTDFQRPIETSYRGETIHFHIDEGMHSRLVELARKNGVSLFMVLQAGLSALFTRLGAG
TDIPIGSPIAGRNDDVLSDIVGLFVNTLVLRTNTSGDPSFKELLNRVKQVNLAAYENQDV
PFERLVEVLNPVRTRNSHPLFQVMLAFQNTPEATFHVPGLEASLEIQSVGSAKFDLTFEI
SESNGDDGTPNGLHGLLEFSTDLYKRETVQKLIERFILLLDDAATNPDQSIGRLEILTLA
EKNTVLEKWNGGFQIAPEMTLPQLFEKQSHINPNSIAVVFEDKKLTYEELNRKANKIARF
LIAKGIGPDQLVALAMPRSLNMVVSLLAVLKAGAGYLPLDPDYPADRISFMLHDAKPSCV
LTNSEVEIECDEALKVLVDDVKVIAEVEKYSEDNIDEVERIKPLSPSHIAYVIYTSGSTG
RPKGVMIPHQNVVRLLGATDHWFQFDGNDVWTMFHSYAFDFSVWEIWGPLLYGGRLVVVP
HTVSRSPKEFLQLLVKEKVTVLNQTPSAFYQLMQADRENEEIGQKLSLRYVVFGGEALEL
SRLEDWYSRHPHNAPKLINMYGITETTVHVSYIELDETIVSLRANSLIGCSIPDLKVYVL
DNYLQPVPPGVVGEMYVAGAGLARGYLGRAGLTAERFIADPFGKPGTRMYRTGDLARWRK
DGTLDYIGRADHQIKIRGFRIELGEIEAVIMKHPKVEQVAVIVREDQPGDKRLVSYIVAS
NDEEIDTNEMRQFASGSLPDYMVPYAFVVVNELPLTPNGKLDRKALPAPEFIASSSSRGP
RTPQEEMLCDLFTEVLSVPQIGIDDGFFDLGGHSLLAVQLMSRIKEALGVELNIGTLFAA
PTVAGLAERLEMGNGQSALDVLLPLRASGDHLPLFCVHPAGGLSWCYAGLMKSLGTDYPI
YGVQARGIAKNEELPKTLEEMAADYLKHVREVQPHGPYRLLGWSLGGNVVHAMAAQLQNG
GEEVELLVMLDSYPGHFLPNTEAPTEEEALIALLALGGYDPDNMDGKPLTMESAVEILRK
DGSALASLEEETILNLKETYVNSVGLLGKYVPKVYNGDILFFRSTVIPDWFDPISPNTWL
NYLDGQIVQHDIDCRHKDLCQPGPLTEIGQVLAKYLQNKKGVSRV

Enzyme Prediction     

No EC number prediction in MGYG000001712_02235.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
NF038078	NRPS_MxcG	0.0	5	1043	1	1049	myxochelin non-ribosomal peptide synthetase MxcG.
cd17643	A_NRPS_Cytc1-like	0.0	1533	2026	1	450	similar to adenylation domain of cytotrienin synthetase CytC1. This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
PRK05691	PRK05691	0.0	1	2107	670	2772	peptide synthase; Validated
PRK12316	PRK12316	0.0	8	1265	49	1294	peptide synthase; Provisional
cd05930	A_NRPS	0.0	1533	2026	1	444	The adenylation domain of nonribosomal peptide synthetases (NRPS). The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QND46664.1	0.0	438	2376	986	2929
ACX49739.1	3.42e-257	11	1767	12	1816
BAZ00088.1	1.91e-251	972	2377	2147	3562
BAZ75991.1	1.91e-251	972	2377	2147	3562
BAY90071.1	1.11e-250	972	2377	2138	3553

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U89_A	0.0	438	1506	3	1071	Crystalstructure of a cross-module fragment from the dimodular NRPS DhbF [Geobacillus sp. Y4.1MC1]
6MFZ_A	1.47e-278	465	2107	211	1794	Crystalstructure of dimodular LgrA in a condensation state [Brevibacillus parabrevis],6MFZ_B Crystal structure of dimodular LgrA in a condensation state [Brevibacillus parabrevis]
6MFY_A	5.58e-264	465	2030	211	1716	Crystalstructure of a 5-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis],6MG0_A Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis],6MG0_B Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis]
6P1J_A	7.53e-210	1051	2026	1	964	Thestructure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae],6P1J_B The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae]
6OYF_A	2.42e-174	1054	1938	1	873	Thestructure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo1 serine module [Eleftheria terrae],6OZV_A The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo1 serine module in complex with AMP [Eleftheria terrae],6P4U_A The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo1 serine module in complex with Mg and AMP [Eleftheria terrae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39845	0.0	9	2110	6	2077	Plipastatin synthase subunit A OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsA PE=1 SV=2
P45745	0.0	1	2377	1	2374	Dimodular nonribosomal peptide synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=dhbF PE=1 SV=4
P39846	0.0	9	2110	11	2076	Plipastatin synthase subunit B OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsB PE=1 SV=1
Q04747	0.0	4	2118	1053	3112	Surfactin synthase subunit 2 OS=Bacillus subtilis (strain 168) OX=224308 GN=srfAB PE=1 SV=3
P27206	0.0	9	2121	6	2092	Surfactin synthase subunit 1 OS=Bacillus subtilis (strain 168) OX=224308 GN=srfAA PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000055	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001712_02235.