CAZyme Information: MGYG000001705_01616

Basic Information

• Species: Citrobacter portucalensis
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Citrobacter; Citrobacter portucalensis
• CAZyme ID: MGYG000001705_01616
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
552	MGYG000001705_1|CGC23	62668.87	9.3478

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001705	4942442	Isolate	not provided	not provided

• Gene Location: Start: 1699948; End: 1701606 Strand: -

Full Sequence      

MKPAARYTLAFIALIALYFILPINSRMLWQPDETRYAEISREMLNSGDWIVPHFLGLRYF
EKPIAGYWLNSIGQWLFGANNFGVRAGAIFATLLTALLVAWLAMRLWQDKRTAVLSSAIF
LTFFSVYSIGTYAVLDPIIALWLMAGMCCFWEGMQATTRTRKIIAFLLLGLTCGMGVMTK
GFLALAVPVLSVLPWVFAQKRWKDLILYGLLAVIGCIAIVLPWGIAIAQREPDFWHYFFW
VEHIQRFAQDDAQHKAPIWYYIPIFVAGTLPWLGLLPGALRTGWRERDVPRSALYLLSWI
IMPILFFSIAKGKLPTYILSCFAPLAILTARFGLQAAEKDSASLRINGWINVLFGVVGIV
ATFIVSTLGPLKTPVWSHIETYKVFCAWGIFIIWTLFGWYTLNNREKNWKFAALCPLGLA
LLFGFSIPDRVMESKQPQFFVEMTKESLEPSRYILTDSVGVAAGLAWSLKRDDIMMYGQT
GELKYGLNYPDAQGRFVSRENFADWLAQHRQQGNITLVLSVGKDEDINQLAIPPADYIDN
QGRLVLIQYRPK

Enzyme Prediction     

No EC number prediction in MGYG000001705_01616.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	5	524	1.2e-156	0.9648148148148148

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK13279	arnT	0.0	1	551	1	552	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
COG1807	ArnT	1.04e-110	12	525	14	535	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
pfam02366	PMT	6.58e-47	4	238	1	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
pfam13231	PMT_2	2.26e-09	62	225	2	159	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUV44554.1	0.0	1	552	1	552
AVD78790.1	0.0	1	552	1	552
ATX92408.1	0.0	1	552	1	552
AWV26145.1	0.0	1	552	1	552
QNM26103.1	0.0	1	552	1	552

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	6.09e-38	4	388	30	412	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B7LM74	1.21e-315	4	552	3	551	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CCUG 18766 / IAM 14443 / JCM 21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73) OX=585054 GN=arnT PE=3 SV=2
B4TBG8	2.18e-315	1	552	1	548	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Salmonella heidelberg (strain SL476) OX=454169 GN=arnT PE=3 SV=1
B7UFR9	6.40e-313	4	552	3	550	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) OX=574521 GN=arnT PE=3 SV=1
B5EZI0	3.26e-310	1	552	1	548	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Salmonella agona (strain SL483) OX=454166 GN=arnT PE=3 SV=1
B4TPI4	6.57e-310	1	552	1	548	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Salmonella schwarzengrund (strain CVM19633) OX=439843 GN=arnT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000012	0.000015	0.000004	0.000000	0.000000	0.000003

TMHMM  Annotations     

start	end
7	29
82	104
113	135
176	198
205	227
258	280
293	310
315	334
347	369
384	402
409	428