CAZyme Information: MGYG000001560_00403

Basic Information

• Species: Massilioclostridium coli
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium coli
• CAZyme ID: MGYG000001560_00403
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1302	MGYG000001560_1|CGC6	142568.13	4.0369

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001560	2985330	Isolate	not provided	not provided

• Gene Location: Start: 485230; End: 489138 Strand: -

Full Sequence      

MKNKKWAKVLSALVATSLTMTTLMASINVSATPDTNVNLKDLTVEYVDSPEGIDVEAPRF
GWKMDSNLVGAEQTAYQIVVKDTDGNLAWDSGKVNSDNSIAVEYASKTPLAPETDYVVSV
TSWDNYGNQATADTTFSTGVDGWDDAKWVSPGETDYAVAMMRSPKQTIQDKTIKNAKLYM
TALGDYKAYINGQLVESEKGETAFDPGWVNYNNYIQYQTYDVTDYISGTDLVLSAELGRG
WYKSSISAKGYKGVAIGGEGDLDLAMIGKLVIEYEDGTEQTIATDDTWEYNKNGPIYNHD
FYTNGSFDPNATTDPDNFVDGVKWGGEKYDASKEVPGWNLPSFDTTQSEDWNDVRIITYN
GDIISNNGAAVAYYNEENNQYPQTSSFTYKDSEINYSKEEGGTSEHVLGEVVRHNVDLMN
EEVTIQPGERLIVNLGQNMAGVLETTMEAEPGTTVVFSHAEMLNDGNKNPDMPRGGSTGP
DGTIYTASLRGEKTAVYKFGEEAEVTYRPDFTFFGFQYVQITATKPVTIKNVVGVPITSA
TKQTGNITTNNADLNQLFSNVLWSQRSNFLSIPTDCPQRNERCGWTGDVQLFAGTGTFNF
DSVAFLQNYQAICDDQAASYNDLYTAVMPAQQGYGKNVSSGWSDVGIVLPWTLYQQTGDI
SIIEESFDQMDAYMDAIGPNGEGYNENWYGDWVSLQACSIQYLNLCYRAYDAQLMAKMAE
ALGYQEKVDKYNQIFDETKAFFFEKYVAENGDILSATADGKSEGDTYHTEYVDNSQTAIS
WALKLGFYKDEAHRQYIISKLLESIKNEDGSFRPGYAEDTLAVGFLGLNVILPSLSGNGQ
REYAYKLLQQNQFPSWLYSVEQGATSIWERWNSYSKENSFMEPGMNSFNHFSFGACLEWM
YQEMAGISRDEANPGFKHIDLTPSIDTTGGINDVQASYDSYYGVIESGWTAENGKLTTYN
TTVPANTTATLYLPVEASAVENFTNITGIEFTGMTIHNDMEVASFRVAAGSYNFAVNDGV
LTVSLADGYVPAVETNKTILNDVIDYADAQMQDEAYQNVIPMVRETFEAALTNAKNVAAD
ITADQDTVNNAWRTLLNEIHKLGFVQGDKASLGSLISAAGSIETNLDRYVEAGKQEFVDA
LKAARAVYEDDNAMQAEIDTVSSALLDAMMELRFKADKSVLEDVLAEASKLDASAYTAES
YSVLEAAVNNANAVLADENATQDDVDLAVNAVRTAIDGLVAVDGTPAETPTDNGNTVDGT
QTGQESTTTKANAAKTGDFAPIAGIAALAVAGAALAITRRKK

Enzyme Prediction     

No EC number prediction in MGYG000001560_00403.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	426	973	2.6e-135	0.9900793650793651
CBM67	146	357	8.4e-25	0.9488636363636364

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	4.46e-92	542	902	1	338	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	9.78e-40	171	358	1	154	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	1.77e-18	427	522	4	85	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	1.62e-17	906	977	1	67	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam07554	FIVAR	1.08e-04	1181	1240	3	69	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYA74416.1	9.70e-256	39	1024	35	1001
QXE01526.1	1.34e-210	157	1037	332	1195
ACX65306.1	1.77e-204	36	1014	4	1004
QUC58338.1	2.90e-200	39	1019	30	1009
QKZ16767.1	6.54e-200	39	1012	51	1030

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	1.78e-111	43	1016	10	1030	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6GSZ_A	7.69e-88	37	974	2	847	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]
6I60_A	1.33e-86	41	1014	34	939	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	7.58e-111	43	1016	10	1030	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	4.25e-105	40	1014	36	920	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	1.03e-80	49	980	42	885	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	1.73e-53	35	998	28	923	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000373	0.998760	0.000202	0.000246	0.000208	0.000184

TMHMM  Annotations     

start	end
9	31
1279	1298