CAZyme Information: MGYG000001542_05019

Basic Information

• Species: Paenibacillus_A sp900069005
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A sp900069005
• CAZyme ID: MGYG000001542_05019
• CAZy Family: GH46
• CAZyme Description: Chitosanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
332	MGYG000001542_44|CGC15	36856.37	7.9172

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001542	5846629	Isolate	not provided	not provided

• Gene Location: Start: 831582; End: 832580 Strand: +

Full Sequence      

MKMTPEKGTHPKGRFTAIMLSFALLSSMVSFSGPVQTKAYAELLENNTDTEATQTLTLTE
KQTNAQEISPMAAETPESKFSPQTLQFLKTNTGLDAEQWDNIMKLVNKPEQDDLNWTKYY
GYCENIDDDRGYTIGIFGATTGGPNDEGPDAPELFKEYDRIKGASNPSVEGALKRLGING
KMKNGILVISDSNSTFVKKINSLQNDSAWREAMWRTFYNVYIKYSVDQAKKRGFNSALTI
GSFVDTALNQGATGDSGSLQGILSRSGSSKDEKTFMTKFYAERTKVVDTNQYNTPPNGKN
RVKQWSTLLSMGETDLKNADAAIKQVTNWKLK

Enzyme Prediction     

EC	3.2.1.132

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH46	93	317	4e-77	0.9954954954954955

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd00978	chitosanase_GH46	3.95e-63	96	319	1	221	chitosanase belonging to the glycosyl hydrolase 46 family. This family is composed of the chitosanase enzymes which hydrolyzes chitosan, a biopolymer of beta (1,4)-linked-D-glucosamine (GlcN) residues produced by partial or full deacetylation of chitin. Chitosanases play a role in defense against pathogens such as fungi and are found in microorganisms, fungi, viruses, and plants. Microbial chitosanases can be divided into 3 subclasses based on the specificity of the cleavage positions for partial acetylated chitosan. Subclass I chitosanases such as N174 can split GlcN-GlcN and GlcNAc-GlcN linkages, whereas subclass II chitosanases such as Bacillus sp. no. 7-M can cleave only GlcN-GlcN linkages. Subclass III chitosanases such as MH-K1 chitosanase are the most versatile and can split both GlcN-GlcN and GlcN-GlcNAc linkages.
pfam01374	Glyco_hydro_46	6.20e-24	104	286	1	178	Glycosyl hydrolase family 46. This family are chitosanase enzymes.
cd16889	chitinase-like	1.23e-11	102	249	1	105	chitinase-like domain. This family includes proteins such as chitinases, chitosanase, pesticin, and endolysin, which are involved in the degradation of 1,4-N-acetyl D-glucosamine linkages in chitin polymers and related activities. Chitinases are enzymes that catalyze the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. Chitosanase enzymes hydrolyze chitosan, a biopolymer of beta (1,4)-linked-D-glucosamine (GlcN) residues produced by partial or full deacetylation of chitin. Pesticin (Pst) is a anti-bacterial toxin produced by Yersinia pestis that acts through uptake by the target related bacteria and the hydrolysis of peptidoglycan in the periplasm. The dsDNA phages of eubacteria use endolysins or muralytic enzymes in conjunction with hollin, a small membrane protein, to degrade the peptidoglycan found in bacterial cell walls. Similarly, bacteria produce autolysins to facilitate the biosynthesis of its cell wall heteropolymer peptidoglycan and cell division.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AZS18150.1	5.39e-212	19	332	1	314
QOS79836.1	9.96e-149	18	332	7	290
AXJ99406.1	1.54e-148	1	332	1	342
ALA43323.1	7.78e-147	12	332	3	334
QAV19074.1	8.02e-147	77	332	60	315

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1QGI_A	3.08e-139	76	332	3	259	ChainA, Protein (chitosanase) [Niallia circulans]
2D05_A	6.19e-139	76	332	3	259	ChainA, Chitosanase [Niallia circulans]
5HWA_A	8.79e-139	76	332	3	259	ChainA, Chitosanase [Niallia circulans]
7C6C_A	1.28e-07	120	328	27	234	ChainA, Chitosanase [Bacillus subtilis subsp. subtilis str. 168]
7C6D_A	1.28e-07	120	328	27	234	ChainA, Chitosanase [Bacillus subtilis subsp. subtilis str. 168]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P33673	3.19e-139	69	332	38	301	Chitosanase OS=Niallia circulans OX=1397 GN=csn PE=1 SV=2
P33665	1.55e-07	81	289	32	236	Chitosanase OS=Streptomyces sp. (strain N174) OX=69019 GN=csn PE=1 SV=1
P48846	3.75e-07	95	287	48	234	Chitosanase OS=Nocardioides sp. (strain N106) OX=47919 GN=csn PE=3 SV=1
O07921	9.00e-07	120	328	62	269	Chitosanase OS=Bacillus subtilis (strain 168) OX=224308 GN=csn PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.007493	0.988256	0.002933	0.000681	0.000357	0.000257

TMHMM  Annotations     

start	end
13	35