CAZyme Information: MGYG000001466_01113

Basic Information

• Species: Providencia alcalifaciens
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Providencia; Providencia alcalifaciens
• CAZyme ID: MGYG000001466_01113
• CAZy Family: GT44
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2995		340168.09	8.4784

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001466	4044196	Isolate	not provided	not provided

• Gene Location: Start: 69900; End: 78887 Strand: -

Full Sequence      

MRVNTISLPSQQERNPVEKTNTPNAINRNNKEKITHEPFINRITKVKNTSTDEVIAIGKL
IQEEIRKTINPVSPLPVSTTGNWKSAIMHRLGLLMSGDSSQISLIDSNNAHQPKFTSVIP
QASSLPFSVDVHHPYNEQTSTTSISSVQYVKVKKIKKRSLDNIEKNNMETNKNKNVKYSL
SKDNIENKIFNNSQNKQIPHHLLLEPLKKSINSYNSLSEKNSREGIELLKEQANLLSRLH
EELNEKSNGENYSNERSNILKLISEVQNEYMSHKVEIEKLIHVVWVAGAPPESITKYAHA
YKNAYPEFTFNLWIDPNAMSAYLFNKEIKEIAFDNAKHEVINLLTPKELETLKTKTDLTP
EFNEKLKDNFEYHLLKCILRLQDAVMRYAYVKGVLTFNDQNRLDFLKEVLSYDDEKINKF
KSKLQDNITKLNVIEKKLAHIFGPDKIKIHNVNHLPEMKIVNYKQNYQKELILRANYAAA
SDMLRTYILKNHGGIYADYDVTPGYTQGVYKIIQENSQDFDFLEKEEHRRALNDELLTLV
SGEPSSELKNKLSSDDKNRLNIILNKIKDQKDEIKVFAPINTEVIRDSMLMSKRHQFWGT
VWNVRGNNNFLATHKGSRVMDFVIDGQKHAYQEIWGIREQLRREDIGKQHSYYNPNNNDH
LEPLRAREKVEAKLFVSDLESNDMKLRKKENLKTLEKDLTEYGKLLALEENGVKKKDIRA
TPEFLKGYKDSNAVNSIPEFKREMNIKNIISLMKKYEKNLSPEQKGALAYEVESRALAVT
FQPRVEEYHQLFEKVKSDGELDPSAKERLIPQLFLLNLVGDGFGGRCDPLAMLLLTEKFL
ESKNKQGLTESLVEKLYSTAAVLSEPIRYTDTEVEKAKQLINVLARLHAKNPMYSTNEKV
WKDKKENQSLNEIIKLLHETNNEPILLKLEAPGHAMAAWSIKNKENRIYGFYDPNGGLVE
FSSINKLELYIHGLFEKSGLNKAIKYHLNIKDDKKTFVFNNVITLDGEILSKYKTAPNDK
TLKEILEISIFDSKAQNTKKVKKQKIIFDPKKYNPGSLFSHYRMDGIIPKKYSTLQITGP
DAIMRSIKNYYNSLGALGQCRLDQSDNRFKGLGESTFVGNLKEISGTSHKHYDWVNQKSV
GINDITPDDESTWLGKKINIKETFANINSITRDNAFEKITPTGLDLKKLQIGWPSELKQK
LNAEWPTLEKDYNQLISSDYLDLDKLSDIDRKIYQYLLLSENSLVRWVGISLSDQLYEKI
NKSSIPIENKAHYLLTDINKKLNENQKSINSILASNHATKIIIWESDFTNKLLIAKELTI
LTARKKEVMELIKNKNKNLNNELNQYCSLKNKEQLETITNTEKERLSLILTKISDTPKIA
SELAEIEKKLINIPISTTFKINDKEINILNIEDKNSLKNNFNGDGDAITLWDKYITNKDK
IKNALQQDAIKTYGNNRVEFKNILTNLKENELIKKITDYKYSFNDIKEIIEYGILQKESG
VIINSAALAAPSNELVNIILNCTGGNDSDAKILLKKLYDYLFNKDDVHFGPSSYEQSLKV
SFMSVLELLDKKNLSKYFLSVMNQNVSALGVSFSSIEGNLTSDIMIAGMKDGLSNKNTIL
DRMNYLFNILYQINNDIKTGKSITVESIKTIFKQKELGFMLQSDSLIHDYLKKIENTREV
SLTEMIQGFTGRNSFLECVPYITADKYPTITNSLLNEIKSRSPSIYSMSDSTFVEPKMLK
GLGYIGNDSYLTNPILGSKLHDVSISAKYQALQWSDFYGRNALLWQDTAIKFEGKNVKFH
PQILLTPQEGRCMGLAELYLRTNSEEQYNTLQTNLDLASALYQESQNQQSQLSESDKHLL
TSIQSQIEHAQQHGNSKLLLSSSVNKIRLSDFETKSVADYLIKNNIIKLLITTPFHSIII
SKLNEKWRVTDPNFGGVNFSNLEKALSFIEQSINISPEVHRLYTGGDANASIDIHFVPDN
DWNQIVSYDALELTSRPYQSTLEKISTLPITISIKNRNIPLVELYKFGVTVEGKRIDEKN
INGIDDASTKLKINGNLLNDYLSKHHLNEGEVSTIHYLLSSLQYQPDTQKVKLSEVIAGP
SAPTSLLVRLEHQRNKFAAFLKSELTRMSMTLIKNGVDLNSPTTKVIDLKLNRSLSEIDV
SLQTSQGKKSVKLDVSTMGLSFKESFDALQEGFDAMHLDEIMSILGIMQYAKMAATGEHL
TSLDHANHVSDLKTLFDSALGLTLLAVGEKSFGTSVSQIKLETIVATKIQQIATKFGGAT
GSLLTKAATVIKLPLLDTALNLWSLGDAVQTYLNPNISSEERLLAGIDIGFATTYTSLAL
LGTVFPPLALSTIPIYFFQQEVKNLLRHNHHIDMRRKAWLSLEHYLNESSKLTILAKPEK
KLLDLSPNKLLGGVTIDLSVSPPTFNAYRSYNDGRDFGNHAHLSDQQVKESSGYAKACTS
TLDIPEIHLFGSIDKVICNDNAKHEYQLAKGYANRLWPPTFPVIPKGDYTTVILGYSARI
IAYTEAKRMNDGTYKELARTDHVLMHKDHKSTIITCSDNFTTFAIPPLEKELFLLKNQDI
LHAMKESWFTLKGGKNGMILQTNGIGNVDFQGQPHTQNILSFKNLGQGFSVHVNLNQNSR
QTVVRSLLPYLTHHSSKMMDLIQNNINTLIGSDYGYDTFIGNQYDNHFTLGAIGGTVHLG
GGNNVVEIPVPDTKTKVYRTSIHLSPNSGMQYFKYKGGINDIDHITYNDDSIYLFFDKNR
NLDAKRIEIHAHKDGDISEYVNKLVFSTEDGLNLKLTEDKKTFTLESANMTSWRKYHKNN
ELDYELIINSLYESYCKKNTCIFFDELMKIFVTNKNIECIINKPDISFNVPTNYSSITYG
ESGVHYISNAINSEPTTIILKNSASKIEHIDISPLLVPVSGEKIAVYAKVSGSKLIITVH
KGSHQKEYTLLSSDSSKLDSSLSRLEFSNSTYLTLLDIYKLVSTSQGNVLIFECN

Enzyme Prediction     

No EC number prediction in MGYG000001466_01113.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT44	415	513	1.2e-25	0.98

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
NF033479	Efa1_rel_toxin	0.0	1051	2920	693	2552	LifA/Efa1-related large cytotoxin. Members of this family are large and almost certainly multifunctional proteins found in various pathogens from genus Chlamydia, about 3000 amino acids in size and related to lymphostatin (Efa1/LifA) from enteropathogenic Escherichia coli. Roles have been suggested for Efa1 (EHEC factor for adherence) in adhesion, so some members have been annotated as adherence proteins rather than cytotoxins.
NF033479	Efa1_rel_toxin	9.99e-99	47	639	13	664	LifA/Efa1-related large cytotoxin. Members of this family are large and almost certainly multifunctional proteins found in various pathogens from genus Chlamydia, about 3000 amino acids in size and related to lymphostatin (Efa1/LifA) from enteropathogenic Escherichia coli. Roles have been suggested for Efa1 (EHEC factor for adherence) in adhesion, so some members have been annotated as adherence proteins rather than cytotoxins.
pfam12919	TcdA_TcdB	1.75e-43	279	634	1	316	TcdA/TcdB catalytic glycosyltransferase domain. This domain represents the N-terminal glycosyltransferase from a set of toxins found in some bacteria. This domain in TcdB glycosylates the host RhoA protein.
cd20495	C58_PaToxP-like	6.76e-41	804	1006	1	179	peptidase C58 domain of Photorhabdus asymbiotica toxin PaTox and LifA/Efa1-related large cytotoxin, and similar proteins. This subfamily includes the cysteine protease domain of Photorhabdus asymbiotica toxin PaTox, a large virulence-associated multifunctional protein toxin. This domain is similar to AvrPphB protease found in Pseudomonas syringae, a C58 protease. Mutation studies show that this domain enhances cytotoxic effects of the toxin, and therefore is essential for full PaTox activity. Also included in this family is the enteropathogenic Escherichia coli (EPEC) factor for adherence/lymphocyte activation inhibitor (efa1/lifA) gene which is strongly associated with diarrhea. Efa1/LifA proteins are important for A/E lesion formation efficiency in EPEC strains lacking multiple effectors. This domain contains the invariant C/H/D residues conserved in the C58/YopT family.
cd20495	C58_PaToxP-like	8.83e-28	1794	1971	1	170	peptidase C58 domain of Photorhabdus asymbiotica toxin PaTox and LifA/Efa1-related large cytotoxin, and similar proteins. This subfamily includes the cysteine protease domain of Photorhabdus asymbiotica toxin PaTox, a large virulence-associated multifunctional protein toxin. This domain is similar to AvrPphB protease found in Pseudomonas syringae, a C58 protease. Mutation studies show that this domain enhances cytotoxic effects of the toxin, and therefore is essential for full PaTox activity. Also included in this family is the enteropathogenic Escherichia coli (EPEC) factor for adherence/lymphocyte activation inhibitor (efa1/lifA) gene which is strongly associated with diarrhea. Efa1/LifA proteins are important for A/E lesion formation efficiency in EPEC strains lacking multiple effectors. This domain contains the invariant C/H/D residues conserved in the C58/YopT family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVF62353.1	0.0	29	2993	14	2971
AMG08231.1	0.0	29	2993	14	2967
AMG29389.2	0.0	125	2995	1	2868
QST64945.1	0.0	39	2993	27	2972
QST74263.1	0.0	39	2993	27	2972

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6II2_A	1.80e-83	690	1033	315	662	Crystalstructure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II2_B Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II2_C Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II2_D Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus]
6II0_A	4.57e-83	695	1033	5	347	Crystalstructure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II0_B Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II0_C Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II0_D Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II6_A Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O in complex with a human ADP-ribosylation factor 3 (ARF3) [Vibrio vulnificus],6II6_B Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O in complex with a human ADP-ribosylation factor 3 (ARF3) [Vibrio vulnificus]
7POG_A	9.75e-13	210	632	29	407	ChainA, Toxin A [Clostridioides difficile]
3SRZ_A	1.05e-12	210	632	29	407	ChainA, Toxin A [Clostridioides difficile 630],3SS1_A Chain A, Toxin A [Clostridioides difficile 630]
4DMV_A	1.06e-12	210	632	44	422	ChainA, Toxin A [Clostridioides difficile],4DMW_A Chain A, Toxin A [Clostridioides difficile]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0A2S3R7M0	9.47e-79	690	1033	3215	3562	Multifunctional-autoprocessing repeats-in-toxin OS=Vibrio vulnificus OX=672 GN=CRN52_02910 PE=1 SV=1
P0DUB4	6.84e-12	277	593	94	391	Cytotoxin-L OS=Paeniclostridium sordellii OX=1505 GN=tcsL PE=1 SV=1
P16154	1.19e-11	210	632	29	407	Toxin A OS=Clostridioides difficile OX=1496 GN=tcdA PE=1 SV=2
Q46342	3.43e-11	277	593	94	391	Cytotoxin-L OS=Paeniclostridium sordellii OX=1505 GN=tcsL PE=1 SV=1
T0D3N5	5.87e-11	277	593	94	391	Cytotoxin-L OS=Paeniclostridium sordellii (strain ATCC 9714 / DSM 2141 / JCM 3814 / LMG 15708 / NCIMB 10717 / 211) OX=1292036 GN=tcsL PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000068	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001466_01113.