CAZyme Information: MGYG000001374_02459

Basic Information

• Species: Mediterraneibacter torques
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter torques
• CAZyme ID: MGYG000001374_02459
• CAZy Family: GH89
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1863	MGYG000001374_5|CGC1	208099.29	4.4893

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001374	3092406	Isolate	not provided	not provided

• Gene Location: Start: 12758; End: 18349 Strand: -

Full Sequence      

MQKKIRKIVSSILASCMVISAFAGIAPQKAEAKTTLVNVAVNGTVSTQNGENGSYVIGNI
NDGDPKTSWQTQGTWPATAVIQLDKGRSISKVAVELGEDGDNSTGRTALVTVQYAQNGIT
TDLIDFGSKRMTVDSKEEFTADVPKSATHIYVTLSDPQNSDGSQGAFWPSVQEVEVFEEQ
EEKVSDYNNIANQAKITTDGNENQSEGSANLVDGNDKTLYKFHNEAQTSEKYIALNFDEA
RTMDAFRIAFEHVGDTDSVDFAFEYSILAKKAGDSDYTKIADHQKANRTDNYAQVYKFNA
AQYSEVKIVMHSCKTQGGTQNGWPAVAEFEVYGSEIEVQDTGSIAFKKPVHTPSGKNTAK
NITDGSKKTQWTGAYYPSYVDIDLEKNYNLDTVEVYTPENGYSQYTIYTSMDGRDFDKLA
EKTSKDSCDAEKGEVYQAEGKEARIVRVYMEYNSASTAAVLNEVRVTGKESKSEVQKRPE
INTVKYEESEYNVEITENDTKNEVYGIIERRLGKAYKDWFKLELAENPKKNGYDYFELSM
DGDQVKIKGNDGVSLATGLNHYLKYFCQVNLSQVGDQADMPENKPVVTEKVFKETKAEVR
YSYNYCTLSYSMAFWGEQEWRDELDWLALNGVNVVLDATAQEEVWRRFLGELGYSHEDIK
DFIAGPAYYAWAYMANLSGFGGPVHDSWFEERTELARKNQLIMRKLGMQPVLQGYSGMVP
TNIHDYDKNAEVIEQGEWCSFQRPTMLKTTSSTFEKYAKKFYQCQKEVYGDVSNYYATDP
FHEGGITGGMNASDISEKVLTEMITADKDAVWIIQSWQGNPTTALLNGLDRVEKGTDHAL
ILDLYAEKDPHYDEGRPGAEAYGDEEEFDKTPWLFCMLNNFGGRLGLHGHLDNLANNIPK
VFNETKYIAGIGITPEASVNNPVLYDFLFETIWQDDASQKMEVIDLDTWLDDYATRRYGA
ESESANQAWDILKETVYKASLNGLGQGAPESVVNARPNLTIGAASTWGNAVISYEKGDLE
EAAALLLADYDKLKDSAGYQYDLANVLQQVLSNSAQEYQKGMSAAFSAKDLDSFKTYSEK
FMSVIEDMEKVTGTSEYFLLGRWVEQAKALANNADDFTKELYEFNAKALVTTWGSKNQAE
KGGLKDYSNRQWSGLIGDFYKARWQRWITARTNELSGTDYESNIDWFEWEWEWVRSNKEY
PTTATPQDLKTLGAEILENYSVKNPAANDEFDLPTEGMTATAGSAQPQTGKEGPASYVLD
KDVSTLWHSKYEGDDQNNLWIDIALGESKTVNGLRILPRNGAVNGVIVEYRIEVSNDNGQ
SYHEVATGTWTNDSGWKWAQFDQTEATNVRLYAVRTLSDQAGKNFASAAEIRIMAPKKEE
PQPEQVNKQFLEFLIGYAQSAKEKPEYEHVVPEVKKALDAALAKAVQVKDNEKASQEQVD
EAYTELLEIVHMLDFIGDTTSLKVLVDAVSDKTEKDYTPSTWQPFKSALDAANEVLKNEN
ALDADIEAARTALETAVNGLVKRADFTKLQASVDEAKKAYGDLSGYTEESAAKYTQALTA
AETVLKNADASQTEVDQAKTDLETAISGLTAKPSPDKVDKTALQSYVDFAKKYADKEEEY
TPSTWNAFKAAYDKAQEVLKDEKAAQKEVDDALAKLQKAAVDLEMKPSKEKLEELKKAIK
AAEKKDLSGYTDASVEAYKDVVEKAKTMLSDKETTATEVEDMLKTLKDADKLLVEKDNPQ
EEDKKPSKEQLEALKEAIKAAEKKDLSGYTDASVKAYKDVLEKAKAVLEDKKATAKDVED
MLKALKDAEKLLVKKDDVKEDEDKNPDSQKGQAVQTGDQTPIMFWAFMLVASAVVIFRRR
KRA

Enzyme Prediction     

EC	3.2.1.50

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH89	542	1211	2.9e-214	0.9864253393665159
CBM32	1241	1371	8.6e-18	0.9596774193548387

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam05089	NAGLU	9.24e-148	600	935	1	333	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This central domain has a tim barrel fold.
pfam12972	NAGLU_C	2.75e-84	949	1205	1	250	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This C-terminal domain has an all alpha helical fold.
pfam12971	NAGLU_N	7.03e-28	502	585	1	81	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This N-terminal domain has an alpha-beta fold.
pfam00754	F5_F8_type_C	8.40e-16	1239	1371	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
PTZ00121	PTZ00121	1.14e-08	1492	1842	1280	1594	MAEBL; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM12248.1	0.0	558	1589	2	1030
ABG85709.1	3.28e-224	357	1320	44	1002
ABG84150.1	4.55e-224	357	1320	44	1002
AMN35090.1	1.33e-223	357	1320	53	1011
AQW23250.1	1.94e-222	357	1320	53	1011

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VC9_A	1.10e-230	357	1224	19	881	Family89 Glycoside Hydrolase from Clostridium perfringens in complex with 2-acetamido-1,2-dideoxynojirmycin [Clostridium perfringens],2VCA_A Family 89 glycoside hydrolase from Clostridium perfringens in complex with beta-N-acetyl-D-glucosamine [Clostridium perfringens],2VCB_A Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc [Clostridium perfringens],2VCC_A Family 89 Glycoside Hydrolase from Clostridium perfringens [Clostridium perfringens]
7MFK_A	1.42e-230	357	1224	27	889	ChainA, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124],7MFL_A Chain A, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124]
4A4A_A	1.74e-229	357	1224	42	904	CpGH89(E483Q, E601Q), from Clostridium perfringens, in complex with its substrate GlcNAc-alpha-1,4-galactose [Clostridium perfringens]
4XWH_A	1.22e-86	530	1220	36	711	Crystalstructure of the human N-acetyl-alpha-glucosaminidase [Homo sapiens]
4A41_A	5.68e-23	1233	1375	25	161	CpGH89CBM32-5,from Clostridium perfringens, in complex with galactose [Clostridium perfringens],4A44_A CpGH89CBM32-5, from Clostridium perfringens, in complex with the Tn Antigen [Clostridium perfringens],4A45_A CpGH89CBM32-5, from Clostridium perfringens, in complex with GalNAc- beta-1,3-galactose [Clostridium perfringens],4AAX_A CpGH89CBM32-5, from Clostridium perfringens, in complex with N- acetylgalactosamine [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9FNA3	3.42e-93	542	1220	92	804	Alpha-N-acetylglucosaminidase OS=Arabidopsis thaliana OX=3702 GN=NAGLU PE=2 SV=1
P54802	6.13e-86	530	1220	59	734	Alpha-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=NAGLU PE=1 SV=2
E8MGH9	2.02e-15	1458	1667	1667	1881	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1
Q0TR53	4.06e-14	1244	1382	634	774	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q8XL08	5.32e-14	1244	1379	634	771	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000283	0.998966	0.000219	0.000190	0.000161	0.000142

TMHMM  Annotations     

start	end
7	26
1840	1857