CAZyme Information: MGYG000001374_00903

Basic Information

• Species: Mediterraneibacter torques
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter torques
• CAZyme ID: MGYG000001374_00903
• CAZy Family: GH84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2022		223147.3	4.5586

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001374	3092406	Isolate	not provided	not provided

• Gene Location: Start: 9847; End: 15915 Strand: -

Full Sequence      

MAGRVIKRLLAGVLTVAVVIPGNFVPAQAAEEPQEDYLIYPNPHKVEYQEGDYILGKELN
VIYDKGIDEATKNRLQEAADLKGIEVNEAEQPKEGATNVYVGVHGQDGTAEDYITEEYQP
EDSLFGKTDSYFLASDENVISVLGKDADSAFYGLTTLYHVFAQMDSLTIRNFEIEDYADV
VSRGFIEGYYGNPWSTEDRVNLMKWGGYYKLNAYFYAPKDDPKHRTQWDQLYTEEELANK
IRPLAEAGNESKCRFVYALHPFPQGNHLRFDDNYEADLAKLQAKFKQVIDQGVRQIAILA
DDFWNPGGPNGVRLLNDMTAWLEEVKKQYPDMKMTIPYVPYDYMGNGSSAELQELKKAPA
NVQIVMTGGRVWGEVTNNFTSTFTNNVGRGPFMWINWPCTDNSKKHLIMGGYDTFLHPGV
EPSKIQGIMLNPMQQSEPSKVAIFGNADYAWNIWETKEEADKSWNDAFSFVDHNSAIMND
ASDALRELSKHMINQAMDTRVTPLQESVVLKEKLNAFKEKLEAETLTAEEVDAVIAEFEV
LQKASKTYRAGGNEAIKGQIVYWLNCWDDTTEAAIAYLNGVKSALAGDVSSVISYNTAGK
TAFDRSKTYDFLYVDHQEYAEVGVQHIVPFINTLAEYVSAKAETAVNPDKVIPKFITSRK
DAPTGSKENIFDGDESTSAVYKTPNSLSKDDYIGVEYNKVIDIDSIRFLLGGGKDHFEHA
KLQYMTEEGTWEDLTLTGMENNFAGEFGKVQDINVKEANLPSDLKAKGIRLIATAANVND
CWLEVREIQINKKEEVSEDTERYTGDVTFSGISTQGNDHTAAKMFDGDLSTEMWIAKGPY
SGEGKDTIAADAYIQITFPEAKQIGSIRMTQGKTSSADVFKKVEIQYSTDGQSGWKKAGE
FTNAKDQTVNINVTDKIKGIRLVNKEQTAGWVRIGELDIRAPKNMTTPITYKVIKTERWK
VAQGKETSLYDGDDNTFVWYDPDGDGNSTSDSVLKDDFLGYDLGTEAVLESAHIVVGHTG
GDKLVKYAVETSVDNSTWTPAEGYAEYTGAAEGKDVLNIDLKGVTARYIRIRNLEKKPTW
AKFSEFTVKQKVDQTGTTENIYTNVKDHGMLGTTEAGMSSLQPGTISLKKDQYIGVDLKN
IKAIENIAVNAGENANVKLQSSMNGVIWTDVNAGALEDARYVRLYNAGNETQNVSVQEFK
VTYAFIGEKTVESDFAQKDSANDMRSNGQVGNVFDGKLNTLGKITGTQDAGKKIVFDLGQ
TVNFESFRYYVKETCLDFLRHAKFEVADRKDATDDQWTKILEVGNAEAVQMSANATAKDA
DYLLHDTTNPGNMYAEATGLNVSGRYLRIVPLTTYTERWVELYELQINGGAYMTTESNRD
IISETAEEAGKIPSNVFDGNFATTYKPSAANGSFTYRISEPDQRTIRIIQNGKASNADVQ
AVLYKDGAKQEAAAIGKLNQTINEFAVGKDSQILEVIVTWAEDIPEISIIKTSEKEKAAV
NKDALNEAIKKPIDDKWTADSKRAAEEAKAAAEEIAANEYVTQEVVDLAEKALLAAHKNA
VVKGDVTALENALNNMKAGKENVGAEEAPVYVEIYSARTYAAYESVMNEIREALKDKENV
SEKEAQDLTAKLEKAEKALVYSSIQRELAETELENAVKYNKDDYTTVSYKAYTDAKSALD
AIVKADKTERKNPKEVYTARNTFAQTEEGLVNVAALKAKLAEVGKLDENLYTKDSWKELQ
DAVKNAEAYLENGTKDQVDKAVSDLENAVNGLVEKTEVTVDDVIAEMEKINGKDYTEVSF
GALQDAISKAKADKDQNDPALDQANITAMKEAKAALVSIVDLKAALNEAAQHKAGTYTVS
SYKLLKDAVTNAEPLKVNGTKEEVANAAAAIRAAIKGLDKRAVGLDEYRDSIVLKKPEGY
TEESYAAYKNAYDALMALDSKETTAEMFANAKSAFEAAQAGLVQKPGSNGTGSSSNGTVS
NGAVATGDTVNVSALAGLFLSLLMIAAFMKRKTFLGFFEKDE

Enzyme Prediction     

EC	3.2.1.35	3.2.1.52

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	183	489	2.5e-102	0.9932203389830508

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	4.02e-124	183	488	1	293	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam02838	Glyco_hydro_20b	8.48e-24	39	176	4	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
pfam00754	F5_F8_type_C	2.96e-10	808	934	1	110	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	3.95e-05	662	788	9	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd07680	F-BAR_PACSIN1	7.19e-05	1630	1781	68	214	The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Protein kinase C and Casein kinase Substrate in Neurons 1 (PACSIN1). F-BAR domains are dimerization modules that bind and bend membranes and are found in proteins involved in membrane dynamics and actin reorganization. Protein kinase C and Casein kinase Substrate in Neurons (PACSIN) proteins, also called Synaptic dynamin-associated proteins (Syndapins), act as regulators of cytoskeletal and membrane dynamics. Vetebrates harbor three isoforms with distinct expression patterns and specific functions. PACSIN 1 or Syndapin I is expressed specifically in the brain and is localized in neurites and synaptic boutons. It binds the brain-specific proteins dynamin I, synaptojanin, synapsin I, and neural Wiskott-Aldrich syndrome protein (nWASP), and functions as a link between the cytoskeletal machinery and synaptic vesicle endocytosis. PACSIN 1 interacts with huntingtin and may be implicated in the neuropathology of Huntington's disease. It contains an N-terminal F-BAR domain and a C-terminal SH3 domain. F-BAR domains form banana-shaped dimers with a positively-charged concave surface that binds to negatively-charged lipid membranes. They can induce membrane deformation in the form of long tubules.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM14174.1	0.0	30	1965	28	1802
AXM91157.1	0.0	1	1970	1	1844
ADP36543.1	0.0	1	1970	1	1844
BBA55766.1	0.0	1	1970	1	1844
ALE11990.1	0.0	1	1970	1	1844

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PV4_A	8.06e-211	37	639	32	645	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
6PWI_A	1.40e-115	22	638	18	624	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV5_A	1.90e-52	35	599	38	593	Structureof CpGH84B [Clostridium perfringens ATCC 13124]
2V5D_A	6.37e-51	34	779	7	734	Structureof a Family 84 Glycoside Hydrolase and a Family 32 Carbohydrate-Binding Module in Tandem from Clostridium perfringens. [Clostridium perfringens]
2CBI_A	1.85e-50	34	550	7	511	Structureof the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase [Clostridium perfringens],2CBI_B Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase [Clostridium perfringens],2CBJ_A Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase in complex with PUGNAc [Clostridium perfringens],2CBJ_B Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase in complex with PUGNAc [Clostridium perfringens],2V5C_A Family 84 glycoside hydrolase from Clostridium perfringens, 2.1 Angstrom structure [Clostridium perfringens],2V5C_B Family 84 glycoside hydrolase from Clostridium perfringens, 2.1 Angstrom structure [Clostridium perfringens],2VUR_A Chemical dissection of the link between Streptozotocin, O-GlcNAc and pancreatic cell death [Clostridium perfringens],2VUR_B Chemical dissection of the link between Streptozotocin, O-GlcNAc and pancreatic cell death [Clostridium perfringens],2X0Y_A Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds [Clostridium perfringens],2X0Y_B Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	0.0	4	1640	7	1491	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q8XL08	4.91e-50	1	779	1	764	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	6.50e-50	1	779	1	764	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q89ZI2	6.73e-41	129	471	100	431	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
O60502	4.07e-20	184	443	63	325	Protein O-GlcNAcase OS=Homo sapiens OX=9606 GN=OGA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000255	0.999054	0.000194	0.000173	0.000164	0.000138

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001374_00903.