CAZyme Information: MGYG000001370_03251

Basic Information

• Species: Bacteroides fluxus
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides fluxus
• CAZyme ID: MGYG000001370_03251
• CAZy Family: GH2
• CAZyme Description: Exo-beta-D-glucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
872	MGYG000001370_90|CGC2	100919.58	5.8698

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001370	4330086	Isolate	not provided	not provided

• Gene Location: Start: 150465; End: 153083 Strand: +

Full Sequence      

MHITYIYCIFAENPLSKNMKSKLLYVFITCFSLLYVRANDTSETIILNRGWMFAQSGTDK
WLPATVPGTVHQDLLNHELLPDPFFGTNEKKIQWVENEDWEYKTTFTVTGEQLSRGAAMM
TFEGLDTYADVYLNGALLLKSDNMFVGYSVSVKEVLRQGENKLHVFFRSPVKETLPQWAS
NGFDYPADNDHSDKKLSVFTRKAPYSYGWDWGIRMVTSGIWRPVTLRFYDVAAIGDYHVK
QLSLTDRQAEISNELEINSISSGKEEAEIIVSCSLKDGQPLIVRRPVVLNPGINHVKIPV
EIKDPVRWMPNGWGEPVLYDFTAQVVCRGKTVASEHHRIGLRTVRLVNEKDEHGESYYFE
VNGIPMFAKGANFIPNDILLPSMTDERYATLFRDIREANMNVIRVWGGGTYEDNRFYDLA
DENGILIWQDFMFGCTIYPADPVFLKRVSEEVDYNIKRLRNHACLAMWCGNNEILEGLKY
WGWQSRYTPEVYEEFYRNYDKLFRELLPSKVKEWDEGRSYIHTSPYFSNWGRPDSWNIGD
SHNWGIWYGKKTFESSDTEIGRFQSEFGFESFPEMKTIATFASPEDYEIESEVMTAHQKS
SLGNDLIRTYMERDFIVPEKFEDFVYVGLVLQGQGMRYCFEAHRRNRPYCMGTLYWQLND
SWPVVSWSGIDYYGNWKALHYQAKRAFAPLIVNPIHENGNLNVYLLSDKLEDSAGMTLEM
RLMDFNGRKLQQESVKVEAPANSSVKVFGKPLDEWADEGQRKECYLLLSLKDRQGREIAR
DIHYFVPTKDLNLPRAVVKSKLKVSDGRCEVTLSSSKLAKDVFVQIPYQGARFTDNFFDL
LPGETRKIVITSPEIRKGVVPEISIKHIRETY

Enzyme Prediction     

No EC number prediction in MGYG000001370_03251.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	38	806	3e-104	0.7659574468085106

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	4.36e-108	41	852	9	786	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17786	Mannosidase_ig	2.90e-25	699	790	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
pfam17753	Ig_mannosidase	7.90e-15	795	870	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam00703	Glyco_hydro_2	8.91e-12	232	342	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK09525	lacZ	1.41e-10	102	473	124	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADV44735.1	0.0	19	872	1	854
QCQ41352.1	0.0	19	872	1	855
CBW22258.1	0.0	19	872	1	855
QCT78327.1	0.0	19	872	1	855
CAH07509.1	0.0	19	872	1	855

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	0.0	37	872	1	838	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	0.0	37	872	3	840	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	0.0	37	872	1	838	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	0.0	37	872	3	840	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	0.0	37	872	1	838	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	1.60e-136	47	861	9	833	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
B0YBU9	2.40e-135	47	861	9	833	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
A1D911	9.30e-135	47	861	9	833	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
A1CGA8	2.74e-133	47	861	9	833	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
Q2TXB7	6.33e-130	47	861	9	836	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.040159	0.957377	0.001716	0.000238	0.000228	0.000243

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001370_03251.