CAZyme Information: MGYG000001340_00373

Basic Information

• Species: Weissella paramesenteroides
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Weissella; Weissella paramesenteroides
• CAZyme ID: MGYG000001340_00373
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
655	MGYG000001340_9|CGC2	74603	4.559

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001340	1962181	Isolate	not provided	not provided

• Gene Location: Start: 60226; End: 62193 Strand: -

Full Sequence      

MSHNEMIQLNQVQVTSEFWQRYRNLVVKEVLPYQWEVMNDQANINIADDPQNNGQDNNSH
AIANLKIAAGLKSGHHYGFPFQDTDVYKWLEAAAYSFSYHQDDNLKKMTDELIDLIADAQ
DDDGYLSTYFQIDAPERKFKRLQQSHELYTMGHYIEAGVAYYQATGNQKALQIAERMADC
IDKNFGLKDGQIHGYDGHPEIELALARLFEATQEQRYLDLAHYFLNQRGQNPEFFDEQIK
ADGVDRDLIAGMRDFPRRYYQAAEPIKDQQTADGHAVRVVYLCTGMAMVARHTGDQELLA
ACKRFWNDIVKRRMYITGNIGSTTTGEAFTYDYDLPNDTMYGETCASVGMSFFAKEMLKI
EAKGEYGDILEKELFNGSLSGMSLDGKHFFYVNPLEADPTASKLNPGKSHILTHRADWFG
CACCPANLARLITSVDQYIYTVHDNTILSHQFIANEASFSDGVTVTQTNNFPWQGDIKYH
LENANHKTYQFGIRVPQWSQDEFSVAVNGQNVDATIEDGFIYLTIDQDNVDIELTLNMAT
KLMRSNNRVKANFGQVAVTRGPLVYAAEEADNEAPLWDYHVNTKDDTTYDYQEETLGGVG
VITVPATREQLESTDDALYKSIDDVPNEIDAQLTLVPYYSWANRLNGQMRVWLYR

Enzyme Prediction     

EC	3.2.1.185

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	12	566	4.5e-211	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	1.48e-169	18	655	22	587	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	1.06e-159	11	566	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
cd04791	LanC_SerThrkinase	7.75e-07	157	301	94	226	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.
cd04792	LanM-like	1.58e-04	159	222	602	677	Cyclases involved in the biosynthesis of class II lantibiotics, and similar proteins. LanM-like proteins. LanM is a bifunctional enzyme, involved in the synthesis of class II lantibiotics. It is responsible for both the dehydration and the cyclization of the precursor-peptide during lantibiotic synthesis. The C-terminal domain shows similarity to LanC, the cyclase component of the lan operon, but the N terminus seems to be unrelated to the dehydratase, LanB.
COG1331	YyaL	0.002	80	221	402	560	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATF41724.1	0.0	1	655	1	655
QPI46807.1	0.0	1	655	1	655
CCC56386.1	0.0	1	655	1	656
APS41207.1	0.0	1	655	1	656
AQW21311.1	0.0	9	653	9	651

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3WRE_A	9.70e-259	12	651	3	654	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.43e-258	12	651	3	654	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	4.07e-258	12	651	3	654	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
3WRF_A	4.25e-250	12	651	3	654	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217]
4QJY_A	1.35e-178	12	653	16	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	5.31e-258	12	651	3	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000060	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001340_00373.