CAZyme Information: MGYG000001172_01436

Basic Information

• Species: Fusobacterium sp000235465
• Lineage: Bacteria; Fusobacteriota; Fusobacteriia; Fusobacteriales; Fusobacteriaceae; Fusobacterium; Fusobacterium sp000235465
• CAZyme ID: MGYG000001172_01436
• CAZy Family: GT30
• CAZyme Description: tRNA (guanine-N(7)-)-methyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
640		75457.09	9.3703

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001172	2065216	MAG	Austria	Europe

• Gene Location: Start: 12606; End: 14528 Strand: +

Full Sequence      

MYNLLRKVGLTLYRPFMKEKMKTFIDKRLSQNFSDLKDEEYVWIHCSSVGEVNLSEDLVK
KFYSISRKNILISTFTDTGYENAVKKYSDKKKIKVIYFPIDDKKKINEILNKIKLKLLVL
VETELWPNLINEVNKKNSRIIVVNGRISDRSYPRYKKLKFLLKSMLQKIDYFYMQSEIDR
ERIVSLGADEKKTENVGNLKFSISLEKYSDDEKEEYRKFLNIGDRKVFVAGSTRTGEDEV
ILDVFKKVKNYVLIIVPRHLDRIPKIESLIKENNLTYVKYGDLENNTSTGKEDIILVDKM
GVLRKLYSISDIAFVGGTLVNIGGHNLLEPLFYRKAVIFGKYTQNVVDIAKEILRRKIGF
QVNDVEEFVEAIKNIESGKISDEEINSFFEENKMIALNIVKKENLIMNNIKDEAKDLWKH
FFHSEKSNYNIYMYKLLDYPDYIMYDNDVMKAKKSKWNEYFGNSNPIAVEIGTGSGNFMY
QLAERNPNKNFIGLELRFKRLVLATQKCQKRNLKNVAFLRKRGEELEDFLADNEISEMYI
NFPDPWEGTEKNRIIQERLFETLDKIMKKDGVLYFKTDHDTYYSDVLELVKTLKNYEVVY
HTSDLHNSEKAENNIKTEFEQLFLHKHNKNINYIEIKKLV

Enzyme Prediction     

No EC number prediction in MGYG000001172_01436.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT30	36	203	1e-52	0.9378531073446328

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1519	KdtA	3.80e-145	1	411	10	416	3-deoxy-D-manno-octulosonic-acid transferase [Cell wall/membrane/envelope biogenesis].
PRK05749	PRK05749	9.47e-108	12	377	22	387	3-deoxy-D-manno-octulosonic-acid transferase; Reviewed
COG0220	TrmB	7.23e-56	426	638	9	225	tRNA G46 methylase TrmB [Translation, ribosomal structure and biogenesis].
pfam04413	Glycos_transf_N	9.40e-55	37	203	11	176	3-Deoxy-D-manno-octulosonic-acid transferase (kdotransferase). Members of this family transfer activated sugars to a variety of substrates, including glycogen, fructose-6-phosphate and lipopolysaccharides. Members of the family transfer UDP, ADP, GDP or CMP linked sugars. The Glycos_transf_N region is flanked at the N-terminus by a signal peptide and at the C-terminus by Glycos_transf_1 (pfam00534). The eukaryotic glycogen synthases may be distant members of this bacterial family.
PRK00121	trmB	2.60e-44	435	626	10	202	tRNA (guanine-N(7)-)-methyltransferase; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATV68159.1	0.0	1	640	1	640
AVQ26185.1	0.0	1	640	1	640
ATV69624.1	0.0	1	640	1	640
ATV66724.1	0.0	1	640	1	640
ATV57243.1	0.0	1	640	1	640

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2XCI_A	1.41e-36	14	379	20	347	Membrane-embeddedmonofunctional glycosyltransferase WaaA of Aquifex aeolicus, substrate-free form [Aquifex aeolicus],2XCI_B Membrane-embedded monofunctional glycosyltransferase WaaA of Aquifex aeolicus, substrate-free form [Aquifex aeolicus],2XCI_C Membrane-embedded monofunctional glycosyltransferase WaaA of Aquifex aeolicus, substrate-free form [Aquifex aeolicus],2XCI_D Membrane-embedded monofunctional glycosyltransferase WaaA of Aquifex aeolicus, substrate-free form [Aquifex aeolicus],2XCU_A Membrane-embedded monofunctional glycosyltransferase WaaA of Aquifex aeolicus, complex with CMP [Aquifex aeolicus],2XCU_B Membrane-embedded monofunctional glycosyltransferase WaaA of Aquifex aeolicus, complex with CMP [Aquifex aeolicus],2XCU_C Membrane-embedded monofunctional glycosyltransferase WaaA of Aquifex aeolicus, complex with CMP [Aquifex aeolicus],2XCU_D Membrane-embedded monofunctional glycosyltransferase WaaA of Aquifex aeolicus, complex with CMP [Aquifex aeolicus]
2FCA_A	8.99e-30	454	623	28	197	Thestructure of BsTrmB [Bacillus subtilis],2FCA_B The structure of BsTrmB [Bacillus subtilis]
7NYB_A	8.99e-30	454	623	28	197	ChainA, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168],7NYB_B Chain B, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168],7NZI_A Chain A, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168],7NZI_B Chain B, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168],7NZJ_A Chain A, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168],7NZJ_B Chain B, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168],7NZJ_C Chain C, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168],7NZJ_D Chain D, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168],7NZJ_E Chain E, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168],7NZJ_F Chain F, tRNA (guanine-N(7)-)-methyltransferase [Bacillus subtilis subsp. subtilis str. 168]
1YZH_A	1.17e-26	454	626	31	203	CrystalStructure of the Conserved Hypothetical Protein, Methyltransferase from Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],1YZH_B Crystal Structure of the Conserved Hypothetical Protein, Methyltransferase from Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae]
3DXX_A	2.11e-12	461	604	31	177	Crystalstructure of EcTrmB [Escherichia coli K-12],3DXY_A Crystal structure of EcTrmB in complex with SAM [Escherichia coli K-12],3DXZ_A Crystal structure of EcTrmB in complex with SAH [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8R6G8	0.0	1	640	1	640	Bifunctional glycosyltransferase/methyltransferase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=trmB PE=3 SV=1
P44806	1.97e-52	42	374	53	389	3-deoxy-D-manno-octulosonic acid transferase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=waaA PE=1 SV=1
P0AC75	1.99e-45	42	389	51	394	3-deoxy-D-manno-octulosonic acid transferase OS=Escherichia coli (strain K12) OX=83333 GN=waaA PE=1 SV=1
P0AC76	1.99e-45	42	389	51	394	3-deoxy-D-manno-octulosonic acid transferase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=waaA PE=3 SV=1
P0AC77	1.99e-45	42	389	51	394	3-deoxy-D-manno-octulosonic acid transferase OS=Escherichia coli O157:H7 OX=83334 GN=waaA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000036	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001172_01436.