CAZyme Information: MGYG000001129_00480

Basic Information

• Species: Ruminococcus_C sp000437255
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus_C; Ruminococcus_C sp000437255
• CAZyme ID: MGYG000001129_00480
• CAZy Family: CBM35
• CAZyme Description: Mannan endo-1,4-beta-mannosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
556	MGYG000001129_68|CGC1	61667.41	3.9773

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001129	1878365	MAG	China	Asia

• Gene Location: Start: 3213; End: 4883 Strand: +

Full Sequence      

MKKIGFLLLAAGMLFSTVGCEKIVQVPAVPYATATEEKQAEWEQEVPSYDPIPLNVPEVE
REAVQEVVEAEEETTLPEPLEVDNTRTGFSGVGYVTGLSAEEKASITLTVEVPTAQHYDL
TIVSAADENASSTLQVNDVEVYVLNTSGSGKFIRATVQGIFLEKGENQITLTPQYGSIDL
DCLEIVNNDTLYETEQKADDALSNPDASPGAKRLMAFLAEHYGESVISGQHVSQANQEIE
QIYHTTGKYPAIRFADLQEYSGNGGTPDTATEIADSLAWGQEGGIVGLMWYWNAPIGPAT
VYAKDATFSLKRAVTDELVATCSEEQLRQMATDGTISDECYAIIRDIDLIAEALMPLRDA
DIPVLWRPLHEAGGAWYWWGASGADSYKWLWNLMYSRMTEYHHLNNLIWVWNGQSSEYLV
SEKRYDIASVDAYLEADATYGSRYEQFVALRNMVGNKIMGLSECSTIPDVNLLFRDHAVW
SYFGLWYGKYLLNPTEPYTTAQQLIETYNSEGVLTREDYQLYCEAHPLENAEAGQETAPA
ASESEAQTEETTSSDE

Enzyme Prediction     

EC	3.2.1.78	3.2.1.100

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH26	203	517	9.2e-84	0.9933993399339934
CBM35	70	185	1.7e-17	0.9747899159663865

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02156	Glyco_hydro_26	5.17e-42	204	490	2	286	Glycosyl hydrolase family 26.
cd04086	CBM35_mannanase-like	1.13e-15	81	185	14	118	Carbohydrate Binding Module 35 (CBM35); appended to several carbohydrate binding enzymes, including several glycoside hydrolase (GH) family 26 mannanase domains. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes, including periplasmic component of ABC-type sugar transport system involved in carbohydrate transport and metabolism, and several glycoside hydrolase (GH) domains, including GH26. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB35s belonging to this family are mannanase A from Clostridium thermocellum (GH26), Man26B from Paenibacillus sp. BME-14 (GH26), and the multifunctional Cel44C-Man26A from Paenibacillus polymyxa GS01 (which has two GH domains, GH44 and GH26). GH26 mainly includes mannan endo-1,4-beta-mannosidase which hydrolyzes 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans, and galactoglucomannans, but displays little activity towards other plant cell wall polysaccharides. A few proteins belonging to this family have additional CBM3 domains; these CBM3s are not found in the CBM6-CBM35-CBM36_like superfamily.
pfam16990	CBM_35	1.21e-06	80	186	12	118	Carbohydrate binding module (family 35). This is a mannan-specific carbohydrate binding domain, previously known as the X4 module. Unlike other carbohydrate binding modules, binding to substrate causes a conformational change.
cd04083	CBM35_Lmo2446-like	1.36e-06	86	185	20	125	Carbohydrate Binding Module 35 (CBM35) domains similar to Lmo2446. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes. Some CBM35 domains belonging to this family are appended to glycoside hydrolase (GH) family domains, including glycoside hydrolase family 31 (GH31), for example the CBM35 domain of Lmo2446, an uncharacterized protein from Listeria monocytogenes EGD-e. These CBM35s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. GH31 has a wide range of hydrolytic activities such as alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, or alpha-1,4-glucan lyase, cleaving a terminal carbohydrate moiety from a substrate that may be a starch or a glycoprotein. Most characterized GH31 enzymes are alpha-glucosidases.
COG4124	ManB2	1.47e-06	362	416	180	234	Beta-mannanase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL18231.1	2.65e-152	54	519	58	527
CCO05604.1	2.83e-106	82	517	92	527
BCN30045.1	5.34e-102	72	527	55	515
ABX41451.1	5.05e-99	82	515	65	503
BCJ93286.1	5.12e-94	72	518	56	507

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3ZM8_A	9.21e-67	82	520	27	448	ChainA, Gh26 Endo-beta-1,4-mannanase [Podospora anserina S mat+]
6HPF_A	9.95e-49	204	518	8	309	Structureof Inactive E165Q mutant of fungal non-CBM carrying GH26 endo-b-mannanase from Yunnania penicillata in complex with alpha-62-61-di-galactosyl-mannotriose [Yunnania penicillata]
3WDQ_A	9.34e-40	205	515	37	348	Crystalstructure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus [Symbiotic protist of Reticulitermes speratus],3WDR_A Crystal structure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus complexed with gluco-manno-oligosaccharide [Symbiotic protist of Reticulitermes speratus]
6D2X_A	8.18e-34	202	518	4	333	Crystalstructure of the GH26 domain from PbGH26-GH5A endo-beta-mannanase/endo-beta-glucanase from Prevotella bryantii [Prevotella bryantii B14]
6HF2_A	1.86e-32	202	487	45	315	Thestructure of BoMan26B, a GH26 beta-mannanase from Bacteroides ovatus [Bacteroides ovatus ATCC 8483],6HF4_A The structure of BoMan26B, a GH26 beta-mannanase from Bacteroides ovatus, complexed with G1M4 [Bacteroides ovatus ATCC 8483]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P49425	2.49e-64	82	518	31	456	Mannan endo-1,4-beta-mannosidase OS=Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) OX=518766 GN=manA PE=1 SV=3
G2Q4H7	1.35e-61	82	520	56	477	Mannan endo-1,4-beta-mannosidase OS=Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) OX=573729 GN=Man26A PE=1 SV=1
P55297	6.47e-56	87	518	42	460	Mannan endo-1,4-beta-mannosidase B OS=Piromyces sp. OX=45796 GN=MANB PE=2 SV=1
P55296	6.10e-55	87	518	41	459	Mannan endo-1,4-beta-mannosidase A OS=Piromyces sp. OX=45796 GN=MANA PE=2 SV=1
P55298	2.15e-52	84	518	37	458	Mannan endo-1,4-beta-mannosidase C OS=Piromyces sp. OX=45796 GN=MANC PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000021	1.000033	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001129_00480.