CAZyme Information: MGYG000001063_01901

Basic Information

• Species: Robinsoniella sp900540475
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900540475
• CAZyme ID: MGYG000001063_01901
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2180	MGYG000001063_52|CGC3	238514.46	4.7273

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001063	7637037	MAG	Sweden	Europe

• Gene Location: Start: 85005; End: 91547 Strand: +

Full Sequence      

MGKRRKKLLSMALVVGMVVSNLSPYTNVLAAEPEHVSPGSAETAELSEDPGDALPPGTAK
AIEETESETAPVSQEVTEEVTEAAPETEIITETEPVETEEETMGETQTQLTEETETETTT
EAEKKNANAVNNVDVNGLFAEYYTTSGSGTNVKLNVLKSKGIDYNINYGDLNPKLMLTTG
QDDAAGIRWSGRIRVPETADYTFYGLVDNGIRLWVNGEQLFNYWDGGSWDIAQTSKAVTL
EAGKFYDIKVEYFDYEGGAHATLSWSNNKGMKDKATIPSSAFYLPSNYNGMYIGSIDTSE
ANLKEGEDFKGDVTVNGFGLDQAERFEIVKTSGESLKTPVYADVTSKKNEEAKIVIPNME
TGTYKLKIIQSNTAVISKGLIIVKPGTDEVRTRTERPRADWERDSYVNLNGIWEFDFDAD
EEGKNAGWFKTDQEFTRSINVPFCWESSLSGISDPDYKGQAWYKKTVNVDKSWEGKKIFL
KFGAVDWKCKLWVNGEEVGEHIGGYSAFEMDVTEYMNVGEDNVVTLWVEDKGSYGDDSYP
ALIGKQGRNAPCGYIHTSGIWQTVGMEARSATYLDNAKAVSDIDNATVTYDLDVTTDKDQ
TLTVEYDFASTVYDLENDVDIPTGSVVEGSQEIQVESGANSVELSPIAIANQKLWNYDDP
NLYQGTLTVKDVDGNVLDELSTYFGLRKVEAKYYNEDLGVKYIYLNDEPVYMSGLLDQGF
WEEGIYTAPSEDALKYDILAMKEAGFNMIRKHLKIEDPLQYYWCDKLGMMVWQDMPHATA
MVPSKEGGEALGRKYYEECLDAAMEMNYNHPSIVSVMLFNETWGLYAAYNDNGKNRNVKA
ADGKSTAEWVEYLYNKTKGAYPNMLIEDMSPCNSDHVQPSDLNTYHMYPKSYDGAVGDVE
NRVNNAYVGSENNYKFGFKQDGDPLLNSEYGGVAAYDGDYDISYCFKYMTDIQRRYEKQS
GFVYTEPFDVEYERNGILTYDRQMKIFGYDEVAYGGDMGIKDLVQETYIGIVDSPIKNVK
PGAKIKTKIMAMSWTNDVPENAVVKWRFDGTDIYGNNISTDLAGTLGMDIIPYEKAEASL
SFRVPAQACVGTLTVWIEGPDGAKIAKNFTNIVVADPSSDNKEMVLDNEKGSITMKAAVN
DRKMVTTKGTGSQDYSYTLPENFNLDSLNGMKIIAEASSYKGKIGTDKNHSSYSSEFSQT
AEGRAVASDMTVSVNGVEIDTVYLPDNPRDMRGTLSLNAPYNGATSAGDFGYLVNLSVSK
EKLAEIKAAMGDSKVMKVTYAVKADAANQNGLRIYNSVYGRYAVNPTIILNPEDIEKADQ
VTAVKNIKTTSDNYSAEGVLSGNSNLTVRTGEKEGYIIALANGGSSITVTNKKTNEVIGV
AAELAAGAHHVKVTLFDEQIRVFADNNPEAVINVYDRSGFTGGITVNASKTNPVSDLVVS
PESYEAVKTEIDDTVKDVEVLDDFSDADYTKRYEIMGNAWSGNVVNGALSMGASDQGEKM
IMKDVSMSDGIYEMDIKVTNSAETHGNVGFVFRSSNYNMGPDGANGYYAGIGDRYVQLGR
MSQGWKELAKVTVPELIVGSTHTLRVAVFGSRIQIYVDDAAVPCVDLNDSTYLEGGVAVR
GYRVAATLDNIHVASIPRYSSTFEKGSDEWEANGNWKTVDGAYTSGSKGAYSLVDSNKIK
DALFAADVKPGTEDSVTALLLRTVSGSVGISGYQLVADAKNDKVQIVKSEKGKTTVLAET
GMRLQAGKTYRLTVEMQGSVIKAYKNDSREALIIAEDAAFTAGQIGIMNVNGTTTIDNVA
INNQFIDGELMKPADPKALDEIIAEAQKVDAGKYTADSYSRLKAALEAALDADRYDQQEI
DQAVSAVREALNQLVLKPIDLTVLNNKIAEAKKLDPSKYTADSYARVKSALAAAEAVNKK
DQAAVNAAAKALSDAIAQLKIKPVDLGALNQKIAEAKKLDANKYTADSYAKVRTALAAAE
AVNKNDQAAVNAAVKNLSDAMAQLKVKPAVPTVPKKNAVYTVNSLVYKVTKSSSKSGTVK
VMKAKKKTYTSISVPKTVKLNGYTFKVTEIYKNAFKNNTKLKKVKIADNVSKIGSYAFAG
CNKLKSVTIGKGLKSIGSKAFYNDKLLKSIVIKSTKVSKVDSKAFSGIHKNAYINVPNKK
ASAYKKTFKKGGQAKTVKIK

Enzyme Prediction     

No EC number prediction in MGYG000001063_01901.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	383	885	2.7e-95	0.538563829787234
CBM66	1488	1631	9.5e-24	0.8774193548387097
CBM66	1650	1799	2.9e-21	0.9612903225806452

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	9.82e-40	409	838	15	429	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	5.07e-33	396	821	3	417	beta-D-glucuronidase; Provisional
pfam02837	Glyco_hydro_2_N	2.65e-25	409	564	4	163	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
sd00036	LRR_3	5.15e-25	2050	2159	17	139	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
sd00036	LRR_3	5.97e-24	2066	2179	1	113	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AWV34213.1	2.18e-132	393	1305	15	911
AIQ74895.1	1.89e-131	393	1305	15	911
AWV99395.1	2.92e-127	395	1311	26	907
AIQ24463.1	2.30e-125	393	1305	10	906
QGQ94319.1	5.03e-124	407	1305	29	907

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	2.88e-67	384	991	6	572	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
5Z1A_A	3.36e-25	397	899	16	471	Thecrystal structure of Bacteroides fragilis beta-glucuronidase in complex with uronic isofagomine [Bacteroides fragilis NCTC 9343]
3CMG_A	5.56e-25	407	899	7	452	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
5N6U_A	1.05e-23	407	829	27	457	Crystalstructure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
5UJ6_A	1.13e-23	432	824	51	414	CrystalStructure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],5UJ6_B Crystal Structure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],6NZG_A Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis],6NZG_B Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	4.08e-25	421	825	53	434	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P05804	1.06e-21	409	821	15	413	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
T2KM09	4.31e-21	393	822	41	433	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	6.03e-20	407	893	6	465	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
A7MN76	2.55e-17	395	822	51	472	Beta-galactosidase OS=Cronobacter sakazakii (strain ATCC BAA-894) OX=290339 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000362	0.998830	0.000265	0.000199	0.000183	0.000156

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001063_01901.