CAZyme Information: MGYG000001040_01748

Basic Information

• Species: Prevotella sp900554045
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900554045
• CAZyme ID: MGYG000001040_01748
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
665	MGYG000001040_174|CGC1	73468.73	7.3273

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001040	2860396	MAG	Denmark	Europe

• Gene Location: Start: 9864; End: 11861 Strand: +

Full Sequence      

MKTIKTLFALSFACITASAQHSGYPVTPVPFTATHVSGGFWKHRLDACRDVTVPLAFSKC
EESGRYANFIKAAHPCDTFKVGGFPFDDTDVYKTIEGASYLLQTYPDKRMERYIDSVLVI
VAGAQEPDGYLYTARTMNPKHPHAWAGANRWDAVEGGSHELYNLGHMIEGAVAYYQATGK
RNFLDIAMRYADNVCKTIGAGEGQIVRVPGHQIAEMALVKLYLATGEKIYLDEAKFFLDM
RGRTSHKDEYSQAHKPVVAQDEALGHAVRATYMYSGMADVAALTGDTAYIHAIDRIWDNI
VSKKLYLTGGIGSRASNEGFGANYELPNATAYCETCASIGNVYVNHRLFLFHGDGKYYDV
LERSLYNGVLAGISLKGDAFFYPNPLESAGGYERKPWFGCACCPSNLCRFLPSVPGYVYA
TRDDSLYVNLFMEGTSDLNIGKNKVRLMQKTEYPFDGEIALTIAKGGGNFAMKIRIPGWV
RGDVVPSDLYRFVDNRQTHYTVKVNGKVVESTIDKGYFTINRKWRKGDVVTISLDMVPRL
VAANDKVEADRGMLAIERGPLVYCAEWPDNEGLDIFSILLPRNPKLVVTDRKAPGGGDVI
TAGVQKVAYDGDGRLVTTDAVLNLIPYYAWANRGQGKMSVWLPYEAGAMHLGPALKVGSN
EFLDK

Enzyme Prediction     

No EC number prediction in MGYG000001040_01748.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	35	564	4e-200	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	35	564	3	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	8.92e-171	25	642	13	585	Uncharacterized conserved protein, DUF1680 family [Function unknown].
cd04791	LanC_SerThrkinase	0.006	174	296	98	230	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADY36175.1	0.0	1	665	1	666
QDO67740.1	0.0	20	664	24	669
ALJ59249.1	0.0	20	664	24	669
QMW86046.1	0.0	1	653	1	654
AAO78779.1	0.0	1	653	1	654

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	0.0	23	653	4	634	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	2.82e-142	32	642	14	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.34e-136	32	642	14	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	1.27e-110	36	641	5	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.70e-110	36	641	5	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	6.96e-110	36	641	5	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000892	0.953967	0.044311	0.000329	0.000245	0.000229

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001040_01748.