CAZyme Information: MGYG000001027_00890

Basic Information

• Species: CAG-56 sp900752065
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56; CAG-56 sp900752065
• CAZyme ID: MGYG000001027_00890
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1934	MGYG000001027_60|CGC1	210365.27	5.1002

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001027	2760146	MAG	Sweden	Europe

• Gene Location: Start: 18500; End: 24304 Strand: -

Full Sequence      

MFSKRIPRRLLAAVMSAALAWSAFPALPPVSASQGITESAELTGREANDFSYHRDFANGL
GNLEKISSGGILKAGNGSASMTGDGIFIDQDSVAAKNTEVEFAFNPKNVSCNYGVILRYV
SEEEYLYVGPSSQHNQHYTSWAVYSQSGKLADIEDAGFVLDGRETPYQAKVRVVDDVVTV
FLDHELIYSGVVNGMTENAGKIGFRTTQSSGMDVYGFTQRSVGNPAESTESQGSFSISSS
EMTVSMDQSFPRVISYALAGGASVSGQTKPIHRVEINNTVYDNPAVTAQADDDSVTYHMA
VSAGEISYAFDISYTVNGNVLEMKLLHIDDAQDKIYTINFPEQCMVSLPSTAPNAKLRVN
NYKNETVYDLTSRSAEKGFSATSLAVLSCDSAAAAISGESYKNRKEIAYQTTDCGAYTST
GLWLNEFTYRGVDGEIEIEEPWVKAAVVGDRNNDGKIDYQDGAVALRDLCMKRKTGADIA
VSSWNMIAMNVGSEAQYPFLRILDNIKKIHLATDGFGQNIIIKGYQSEGHDASHPDFANY
NRRAGGLSDLVTLLEESEQYNAKVGVHINHTDVYPEAPQYAKLASGLSAWSWYDTSMGII
RENDCLDTSANGLDGRLSRLFDTDTSHMLDTVYVDVFFGTRWPMYKLVRNISGRGMALGT
EYVDEMVSYSVFAHHIGSAFGGAGNLVRFVNNNQADIFTNHPLFRGADSRANEQIGIDGW
QGAKNLNTALTAFYTKILPNKYLANFPVMQYETDNRAVLGENGEVITEMTNGVNIITKNG
KKIAEGNKMFIPWDPKTEEKIYHYNPDGGASTWELPDSWKDVTSVKVYRLSDEGRNLADT
LTVSAGKITIQAEAGTGYVLCKGDAENTALHAETMDWSTGSPVRDMGFDSHGFGYWKKDE
SASHIVIENNSLGNSHLYIRGTQAGEVSQTITGLTPGETYSASVWTITDEGKKASIRVDT
GTETVENYMTRSNVLYGYHHNDKYQTYAQRMSVRFTAGSDTAVLTLAAEAGNSANSSTDF
DDVRVMACGITDPGSHTYYEDFENVDQGLGIFVSTESDQSHLSQKNPLNPEYTPDVISGT
YSLKVRAGDYMRTLPSTMCFLPNTEYTVSLEYKSAAAEAFTLGVKPDRARESGDTKNAVL
AEAVPTLGNMTKGTLELTFTTGDYDDYYMDITQKNAAEYVIDNVAVDAAVPMTAESLQKL
LASADSMTEKDYTPASWNILTEAIRNARNLLNTQITPSTEDITSVYTTLETAIKNAERYA
DREERTALQTMTNELKKLRSSDYIMGIGWMNLQILLKEADTLAQNSAATSPQISRMMQSL
ETAKSGLTPAVDRSRIHAAIAKAEAVNRSAITDGMELQSFLGAMEDAIKINQTAGVTKEE
IANAAAKLESAYQTLTIKDSSLNGMITAALEKSEVDAAYFPADALSEIQEARTALESLWG
GSGIFAHAFYDIMESLDAALSRKQDTPVLSDSLALESGSFTITCDNENPLSGSEGPASLA
FDGDANTIWHSNYTPVTPLPATIEIDLGAEYPVNQFSYQCRPTGSNGKVTDYSLSYLEAA
SGSWLPILEGGSFVNDTSVEKVSFAPVTTRKIRFVVNQGEGGFGSAAELAVYRKTADKNG
LQIQLDAFRRLDSSQYTPASLAALESLIRQAQVLLEDTDASQESVDALLKQIQAAADGLS
LLPSQTDLQVLKAVIRDAEAEDPAAYKNPEAFLSALADAKKALAAPTDQQAVQNASFSLY
QAHHALIEAGKKPVSEPAPGPAASQSLSAAAIAPIASQTYTGKPLTPQVSVTLGGTALRN
GTDYTLSYQANTNPGKALVTITGKGHYTGTRTASFHIVPKKAVLKKAVKYRTKAIKLTWK
RDKKADGYQICCSTNKKFKKGVRKILVKKNKTVTKIVKKLKSRTVYYVRIRSYKTIDGKK
VYGSFGKAKKVKTR

Enzyme Prediction     

No EC number prediction in MGYG000001027_00890.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	240	775	3.7e-97	0.8217821782178217
CBM32	1492	1609	9.3e-17	0.8709677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14244	GH_101_like	3.89e-82	473	753	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam12905	Glyco_hydro_101	1.78e-68	460	730	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
pfam18080	Gal_mutarotas_3	1.15e-40	241	459	5	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	2.44e-25	738	835	3	110	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.
pfam00754	F5_F8_type_C	8.24e-16	1492	1609	9	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBK62189.1	0.0	51	1754	320	2044
BBK22105.1	0.0	51	1754	320	2044
ATD58436.1	0.0	49	1475	496	1915
ATD54117.1	0.0	49	1475	496	1915
QBJ76357.1	0.0	49	1475	496	1915

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEP_A	2.73e-101	241	1246	16	1177	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QEV_B	2.82e-101	241	1246	16	1177	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
6QFK_A	2.82e-101	241	1246	16	1177	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
6SH9_B	2.82e-101	241	1246	16	1177	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]
2ZXQ_A	3.49e-101	241	1246	31	1192	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A9WNA0	8.98e-99	236	1191	51	1039	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
Q8DR60	8.03e-89	237	1226	334	1456	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
Q2MGH6	3.20e-88	237	1226	334	1456	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
P0DTR4	5.11e-17	1493	1612	526	644	A type blood N-acetyl-alpha-D-galactosamine deacetylase OS=Flavonifractor plautii OX=292800 PE=1 SV=1
Q0TR53	1.44e-10	1356	1614	524	768	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000473	0.998650	0.000205	0.000290	0.000184	0.000155

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001027_00890.