CAZyme Information: MGYG000001018_02250

Basic Information

• Species: Mediterraneibacter sp900751785
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp900751785
• CAZyme ID: MGYG000001018_02250
• CAZy Family: GH136
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1249	MGYG000001018_152|CGC1	134048.54	4.063

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001018	2841260	MAG	Sweden	Europe

• Gene Location: Start: 12240; End: 15989 Strand: -

Full Sequence      

MKKKRVYKAAAGMLAAGMLLGEILSGTVLQAQIVSGAVPSSEGTETAGGEQVQSENKTVV
TRIQAENDAYVTLNPGIGTDMGSSFVVENEAASDEGAHIKTSVIGASVTLRFNGTGVRFY
TKKGNGAGNLSVSVDGNVYDDISEYVSGSAQFKTKVFEAENISSENEDHVLVLTTKTAEN
VGSNFNFDYFEIISEEEEAEYIQSPGDTTYYLDSSAEDGGDGKSEEEAFDSLDDINGYQF
AAGDRILIKAGSEFQGQLYPKGSGTADAPVVIDMYGEGEKPLIDGNGRYSNAPSVSDDGP
FGEAGAAVYLYNQQYWEINNLRVTNWSSDGVDRERSGIRVEASGGGTYRHIYIRNCEISD
IRGYDGQDSIWDVVPENGGTTFYGARTTHRTGGINIVSYTKRDTSAGLGTAGEILDEEPT
IFDDVLIEDNTIENCHANGITTTNVRGELDDKDYRHTNVVIRGNEIRNVQRAGIVPLYTS
GVLVERNLVDTFQQTTKGYGCGIWCDRADNMLFQYNEVCNGQNTMDGMAFNLDDMTENGI
IQYNYSHNNVGGGVMLHVRTNSYNRNNVVRYNLSVNDTRGYAAHQAIIVCVGEDSSTKIE
NAKIYNNTFVNTNTVHPVYQGNEILFANNIFYLPNQGMASKADAYTLGAKTTFINNVFAG
AHSSSEPSGNGNVSVDDPLLAGRLDGTESLEEAMEKAMLSHESPALGIGDTTVLADAETD
FYGNAAVIDGKCNAGIYNGESVDRLPDSGDPDPGTDPEFGEEPSEDEYDVEYIEAEDDAV
IKSGAYTLASGSQSEGHANTHIYFADTGSYAEYTFTGKAISVYTKTGPGAGVATIYLDGE
EAAADDQYTAVQEFNKRAYTVVFEEEGLHTIRVENSGNRNPSSSGNTVNIDCFKVFHGKD
KDVDLTELENVITLADEYAAKIDSYTEDTAEAFWNAYNAAKTVLADTEAEQDEIDAAAAA
LKTAIDGLKLAEEISTAVLEYALELAENVDTEGVADSVVEIFNDRKAAAQDILERTKAGD
PSVTQSMVDESWRGLIEIIQYMSFKPGDMTDLQKVVDLANTMDLTKYLEKGQQEFTDALA
AAEAILEDEFTEQSEINEAWQNLLTAMSELRLKPSKGALEELIASAQSLSTWGVSEETAA
VFRSALARAVSVYEDEQATKEEVVSAEEELQAAIDQMLASAGGSTGTTGSKSTGGGTDTD
GAQNRGSKTSVSGSNAVKTGDAASAGVLLAIMAGSGVLAVCAGRKRKKS

Enzyme Prediction     

No EC number prediction in MGYG000001018_02250.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH136	208	736	3.3e-103	0.9898167006109979

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam13229	Beta_helix	5.68e-07	454	629	6	156	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
cd14251	PL-6	1.95e-04	563	661	261	369	Polysaccharide Lyase Family 6. Polysaccharide Lyase Family 6 is a family of beta-helical polysaccharide lyases. Members include alginate lyase (EC 4.2.2.3) and chondroitinase B (EC 4.2.2.19). Chondroitinase B is an enzyme that only cleaves the beta-(1,4)-linkage of dermatan sulfate (DS), leading to 4,5-unsaturated dermatan sulfate disaccharides as the product. DS is a highly sulfated, unbranched polysaccharide belonging to a family of glycosaminoglycans (GAGs) composed of alternating hexosamine (gluco- or galactosamine) and uronic acid (D-glucuronic or L-iduronic acid) moieties. DS contains alternating 1,4-beta-D-galactosamine (GalNac) and 1,3-alpha-L-iduronic acid units. The related chondroitin sulfate (CS) contains alternating GalNac and 1,3-beta-D-glucuronic acid units. Alginate lyases (known as either mannuronate (EC 4.2.2.3) or guluronate lyases (EC 4.2.2.11) catalyze the degradation of alginate, a copolymer of alpha-L-guluronate and its C5 epimer beta-D-mannuronate.
pfam05048	NosD	0.006	423	629	28	191	Periplasmic copper-binding protein (NosD). NosD is a periplasmic protein which is thought to insert copper into the exported reductase apoenzyme (NosZ). This region forms a parallel beta helix domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYE35145.1	9.28e-169	206	929	34	780
AZG68471.1	4.38e-121	209	636	117	560
QNK55088.1	2.96e-67	208	744	58	545
QJD84649.1	3.58e-66	207	742	254	739
QNK55089.1	1.22e-62	191	746	239	744

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7V6M_A	2.52e-30	208	724	9	564	ChainA, Fibronectin type III domain-containing protein [Tyzzerella nexilis]
5GQC_A	4.67e-26	208	725	18	585	Crystalstructure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_C Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_D Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_E Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_F Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_G Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_H Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQF_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQF_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum]
6KQT_A	1.55e-22	210	555	247	598	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - native protein [Eubacterium ramulus ATCC 29099]
6KQS_A	7.56e-21	210	554	247	597	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - selenomethionine derivative [Eubacterium ramulus ATCC 29099]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH9	1.28e-06	903	1118	1666	1883	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.012225	0.979470	0.007468	0.000315	0.000244	0.000251

TMHMM  Annotations     

start	end
12	34
1222	1244