CAZyme Information: MGYG000001018_01600

Basic Information

• Species: Mediterraneibacter sp900751785
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp900751785
• CAZyme ID: MGYG000001018_01600
• CAZy Family: GH136
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2101		227728.25	3.9776

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001018	2841260	MAG	Sweden	Europe

• Gene Location: Start: 10299; End: 16604 Strand: +

Full Sequence      

MKQRKKILSVILTVAMLFGNASAAFAEEPVSEAIEAGVSMETETPNPDADPDAGSRESES
VTYYVSSLNGSSENPGTSEDQAFSSLLEINDITLKPGDKVLLERGSVFENEYLHIEGAGS
ADEPIIIGCYGDESKPLPQINTNGYGVWHQDYNIDSLDSTTHVLKGDVSSCILLYDVEYV
EISDIAMTNSGNFADGENYSTADRMDRTGVAAVTQNIGTADHIVLRNLDISDIQGNVYNK
HMCNGGIYMVCAQPLDESTGIAKYNDVLIEGCTLSNVNRWGIAVGYTCYWSQFNGAELPD
DVVMEYGSTNVVVRNNYLSNLGGDGITMMYCYQPLVERNVVTAFARDMNNSVYTEPVSQK
NGGKNRGGMLAAGIWPWKCKTPVFQYNECYDAYANQDGQAWDADSGDGCIYQYNYSSNNA
GGSVMFCLQEAVNTVFRYNISQNDLGGVLNLSSNPNGEIYNNVFYIKENVPVNRMRNGTV
NIYNNIFYYTGDEPAPASVCNWNWIRGKWSNNIYYNFSTIPDDPNAITEDPMFVDGGSAP
AGAQISGLVYDRSVFDGYKLQEDSPAIDAGKPVSSAGGQDFFGNELDMMPDIGAYETGLY
VADSNAEIVSTPYMITGEGTRQTINVPSLEKNPTTTEDVLANIEIAPKSEAFIFKGEDEA
GRALVEDGMILRIYAESGVYRDYTIKVKNSYDFVEDYSSVQGNVWFYQYKSKDGTYSNMT
AKDEWGGWMESYENGMSNTWATVARENNTRDGIVSGPLYNRNGADVESAAMAWRAPKAGS
VTLSFGGGSVVGGAKLRGTTSGGPSYLKITKNGEDVIDPINMTESGGALIALESQVLKVE
QGDYIRVECMTAADAENPGFYVTPVVSYNNTGDEEAPSVPGNVRASQIQGAKAVISWEAS
SDNVGVEGYNIYLDGELIATTASSSYQLRGLTEGGEYTVEVDAFDLANNVSERGSVRFTA
EAENLIYTLPEPVELDGTEGGITLDQDVLDASTDLDELTVTAQFVGPTSLGSVISFSQSD
ATANHFHIYANGVTLGYEMRGEWGNYNAAVSCLEPGSGNAIAFVADPDTYTFKLFANGEL
VHTRQLNEGSWRMLSDYAGALDAVTVGWTPRNHTSKYPFVGDILDLNIYSAAFSDEDMTA
YTNIDVPAPEKEPHMFSATGSLFYRIPALLTLSDGSLMAAVDARFGSAADSPNNLDTAVV
FRQAGEEEWEDATIPFHFLDISDKAGAVTNNSASFIDPVIVQADDGTIYLMADAFPYGTG
SARAQAGSGMLTVDGEKYLALTEKGNSVTDMENFTLYIKEDGTVWDSESNTATEYSVDEN
FNLYKNGSALTIQQRDPSLQYNGTRVPMNIFYEDAELQVYRTSYLWMASSSDGGKTWSAP
QILNDLKLEAESFLGFGPGRGYVISQGEYEGRILIPVYSTEGSRGERSSVIYSDDNGKTW
ERGPATTVTNDAALNPGKTSEAQFVELPDGTIRMYARGTTGYVGYSDSADGVNYGPFKED
PQLAYCGNSMVSVINYSKTVDGKPALILSCPEHRENRKDGIIRIGLISENTDPSSAEKYT
VDWKYRYEVNRDEFIYSCLTELPNGDIGLLYETHVDNGAPMNYAEYTLEELLVEEDSVID
SVELSPSQPEPGDEIRVSVTLREPAVNPEDLDTAVLEILYPASGLGKGTLSFEGISEDNL
VLTYSGSLPKSESAYEFLIRMPEWCSHPTVGKKSPVVETADGLINPANKDTLYGMASAEP
EPAPETDKSALEAVIADGDTYAAQIDRYTSETAEVFRNAYDAAKKVFADAEASQEEVDNA
AAALQAAIDSLELAQASGEVSTAILEYALELAENVDTEGVIDSVAEIFNNTKAEAQDLLK
RVQEGDATVTQSMVDQCWQLLIRTMQYMSFRQGDMTDLQKVVDMANSLDLTRYLEEGKKE
FTDALAAAETVLGEEFVEQGEIDQAWKDLLKAMSELRLKPSKEALEALVDQANSLNIEGA
DEETAAVFRSALARAMSVLDNSDAAEDEIAAAESELQAAIDQILAAAEDTSGKLLSDQSG
KSSVDTTVKPAAGAGDETTVAAGSSKAAKTGDTFDLAVWGAVVLLSAGILVSSGYRKKKT
K

Enzyme Prediction     

EC	3.2.1.140

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH136	61	596	3.7e-150	0.9938900203665988
GH33	1158	1596	7.8e-66	0.9210526315789473
CBM40	981	1142	1.3e-21	0.9106145251396648

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd15482	Sialidase_non-viral	2.02e-51	1159	1598	9	329	Non-viral sialidases. Sialidases or neuraminidases function to bind and hydrolyze terminal sialic acid residues from various glycoconjugates, they play vital roles in pathogenesis, bacterial nutrition and cellular interactions. They have a six-bladed, beta-propeller fold with the non-viral sialidases containing 2-5 Asp-box motifs (most commonly Ser/Thr-X-Asp-[X]-Gly-X-Thr- Trp/Phe). This CD includes eubacterial and eukaryotic sialidases.
COG4409	NanH	2.68e-44	1058	1593	174	701	Neuraminidase (sialidase) [Carbohydrate transport and metabolism, Cell wall/membrane/envelope biogenesis].
pfam13088	BNR_2	5.59e-13	1369	1539	86	238	BNR repeat-like domain. This family of proteins contains BNR-like repeats suggesting these proteins may act as sialidases.
pfam02973	Sialidase	5.57e-10	989	1142	24	185	Sialidase, N-terminal domain.
pfam13229	Beta_helix	7.40e-07	265	463	9	157	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCT93619.1	5.82e-144	63	597	37	580
ADO52688.1	4.46e-140	48	964	24	994
ARV05068.1	1.93e-139	63	597	37	579
ALE10905.1	2.98e-138	48	968	24	976
EFR49825.1	1.74e-137	48	964	11	981

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7V6M_A	4.83e-154	61	596	9	576	ChainA, Fibronectin type III domain-containing protein [Tyzzerella nexilis]
6KQT_A	8.13e-130	63	471	247	644	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - native protein [Eubacterium ramulus ATCC 29099]
7V6I_A	8.28e-126	58	597	11	609	ChainA, Lacto-N-biosidase [Bifidobacterium saguini DSM 23967]
6KQS_A	9.08e-126	63	471	247	644	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - selenomethionine derivative [Eubacterium ramulus ATCC 29099]
5GQC_A	8.08e-122	58	599	15	599	Crystalstructure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_C Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_D Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_E Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_F Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_G Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_H Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQF_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQF_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q27701	3.73e-96	962	1594	74	731	Anhydrosialidase OS=Macrobdella decora OX=6405 PE=1 SV=1
P29767	6.67e-89	995	1805	219	1010	Sialidase OS=Clostridium septicum OX=1504 PE=3 SV=1
Q54727	1.38e-62	1007	1594	81	671	Sialidase B OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=nanB PE=1 SV=2
P62576	3.18e-45	971	1598	125	773	Sialidase A OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=nanA PE=1 SV=1
P62575	3.18e-45	971	1598	125	773	Sialidase A OS=Streptococcus pneumoniae OX=1313 GN=nanA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000265	0.998981	0.000186	0.000210	0.000172	0.000157

TMHMM  Annotations     

start	end
7	26
2076	2095