CAZyme Information: MGYG000000927_00829

Basic Information

• Species: Phascolarctobacterium_A sp900545025
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium_A; Phascolarctobacterium_A sp900545025
• CAZyme ID: MGYG000000927_00829
• CAZy Family: GH23
• CAZyme Description: Soluble lytic murein transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
206		24394.02	8.7608

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000927	1752526	MAG	China	Asia

• Gene Location: Start: 66448; End: 67068 Strand: -

Full Sequence      

MAGRKRKSSRNKGNSKIKRYIHLFICFLLLVASYGGWRLWNSDAVQMRFVYMWPYQQDIL
EYSRRNNIDPFLVAAIIKNESGFEPKAVSAVGAVGLMQIMPETGRWIAEQMGLEDYQDND
LYQTKKNIRMGCWYIGELEHEFQRNLALIMIAYNAGRGQTKAWMEENGWDYNFTDLEAIP
YPDTKEYVIKVLHDRDRYYLLYKDRL

Enzyme Prediction     

No EC number prediction in MGYG000000927_00829.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	61	194	7e-28	0.837037037037037

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd16896	LT_Slt70-like	1.07e-73	53	197	2	146	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
cd13401	Slt70-like	4.50e-52	54	205	5	152	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
cd00254	LT-like	5.27e-33	70	192	1	107	lytic transglycosylase(LT)-like domain. Members include the soluble and insoluble membrane-bound LTs in bacteria and LTs in bacteriophage lambda. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
pfam01464	SLT	5.65e-29	59	169	1	108	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.
COG0741	MltE	6.36e-27	1	206	79	287	Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTV76946.1	3.35e-111	4	206	5	205
BBG64618.1	1.92e-95	10	206	5	198
QNP77119.1	1.61e-94	24	206	27	209
AEQ23289.1	6.14e-65	19	202	12	193
ADB46849.1	1.27e-64	12	206	4	196

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MPQ_A	1.01e-24	51	202	412	559	BulgecinA: The key to a broad-spectrum inhibitor that targets lytic transglycosylases [Neisseria meningitidis]
5O1J_A	1.01e-24	51	202	416	563	Lytictransglycosylase in action [Neisseria meningitidis MC58]
6FPN_B	1.02e-24	51	202	422	569	Lytictransglycosylase in action [Neisseria meningitidis MC58]
5O24_A	1.03e-24	51	202	426	573	Lytictransglycosylase in action [Neisseria meningitidis]
5O29_A	1.04e-24	51	202	432	579	Lytictransglycosylase in action [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39434	6.99e-17	28	199	458	625	Soluble lytic murein transglycosylase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=slt PE=3 SV=2
O31976	2.03e-14	51	192	1418	1538	SPbeta prophage-derived uncharacterized transglycosylase YomI OS=Bacillus subtilis (strain 168) OX=224308 GN=yomI PE=3 SV=2
O64046	2.03e-14	51	192	1418	1538	Probable tape measure protein OS=Bacillus phage SPbeta OX=66797 GN=yomI PE=3 SV=1
P0AGC3	2.31e-14	28	199	458	625	Soluble lytic murein transglycosylase OS=Escherichia coli (strain K12) OX=83333 GN=slt PE=1 SV=1
P0AGC4	2.31e-14	28	199	458	625	Soluble lytic murein transglycosylase OS=Escherichia coli O157:H7 OX=83334 GN=slt PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000021	0.000013	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
20	42