CAZyme Information: MGYG000000919_01644

Basic Information

• Species: Niameybacter sp900551325
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Cellulosilyticaceae; Niameybacter; Niameybacter sp900551325
• CAZyme ID: MGYG000000919_01644
• CAZy Family: GH101
• CAZyme Description: Endo-alpha-N-acetylgalactosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1306	MGYG000000919_6|CGC4	149250.04	5.4353

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000919	4175004	MAG	China	Asia

• Gene Location: Start: 120161; End: 124081 Strand: +

Full Sequence      

MKYAADLSKKDVSYWIKTSESTGDIEAKLQREFNRLYMKGKLLCVDEACPVFESYFVDVS
LRITKKGQHVGVVLAYKDDNQWIWIGHKADGKWGYKGANGTEVFGFLGPTLKEGRTYNLK
VKYDQQEITLWVNGQLIFKENIQTDYSCTGKVGLMSWHEENQILVNDLYCQEVEKIVYTP
QEPVLTTIHSEKMVVEIDETFPRVVSYTLSPSGVLLYGQEDVLSQIKINSDTYIPQVTFK
KVSSNEACYSLKVPEIQVELSIGMQVVGNVLDFKVTHIEETGDILVKTLEIPSHSLISVR
SNQDNPQVTATKLWGILTATDEYHTLHEMKEHGIPTEKTMVFINTDTVAASIENNVLYKE
KRVLYEAVTKEEYKKCGVWNGVWTYREIDEEVCELPWCKVVLSGDENGDGKVDWQDAAIG
YRKHMVLPFGHEIARSHVSHIAMDFASLAQYPFLRILDNIKKYHHYTDGFGQMVLVKGHA
AEGHDSAHPDYGGHFNQRAGGIKDFNQLVEGAHGYNAKVGVHINATEYHPEAHGYNPDIL
KKDAEDGWAWLDTSHYVDRKKDIVSGELYRRLEGLKSDAPGLDWVYVDVYMEKNAWNEYK
LASKINDLGLALGTEWPGPMDKWVVWNHWQDIGYDTTGDGLTSKIIRFVSNQVKDAFPHH
PLLKSNDNKGFMGWEGDLPREKDLLYAIEKFFTWSLPCKYMQHFPIQSWAKDKVQFEGGV
SVIGEGEIEVIKKVADEEGVYSKQKVKMPQFTKLYKDGKLIYSGENVQHTGERIQERNNK
LISNNNLVFIPWNPLTEEKIYHWNDLGGTSTWDLPESWTNLKCIKLYKLTSTGRTFVREL
EIVDGRVTLAVLPKTPYVLYKEESVGEETLWGQYSLVKDMSFDSGVFDVWQRSSKSLEVD
HIQMVMEEEVGKGRGYNYLLIKGSQGAQGMVEQKIEGLTPGKTYTASVWVEVFKGRKAIL
GIKDYGGPHVTNYVDVTDVPNYNVNSEKHLTHFQRIKVQFTMPQGESSAILYLEAEVGRP
DSYVKFDDVRLVETEATYKDTHYFHEDFEHVDEGWGPFVYAYDGFCQTHLSESNLPYTKD
VIEGKFSLKTKDEEYAGEIYRTVPGLLRFEKGKKYKVSMDYMAYNEYGKTKDYQYKLVLR
SKEHGEVDTLSEYSLLSTKTNDTPNGPVEVAHMEWTIETGAYKEAYLAVVKDNKEQGILV
IDNLIINEYEGDIVHPEQPTSYCGVQPIEEGIVKTTIGRIPKLPEFIKVKTYTNEVLCVP
VVWEYVSPTKYKEAGTFNVKGKLLGINQEVQTKVEVEEAKKNLEWM

Enzyme Prediction     

EC	3.2.1.97

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	190	861	3.4e-155	0.9971711456859972

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	1.38e-105	415	676	1	265	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	6.50e-97	428	709	2	296	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam18080	Gal_mutarotas_3	1.69e-62	188	414	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	2.32e-23	695	835	2	111	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.
pfam17974	GalBD_like	5.11e-18	1027	1181	1	160	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AQT68159.1	1.49e-271	187	1207	27	981
AMN35754.1	2.31e-255	188	1216	92	1157
AQT69519.1	8.16e-246	188	1125	51	920
AZN39795.1	4.29e-239	70	1202	70	1143
QHW33475.1	5.54e-228	30	1211	37	1212

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEP_A	5.13e-112	187	1123	11	1030	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6SH9_B	5.33e-112	187	1123	11	1030	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]
6QEV_B	5.33e-112	187	1123	11	1030	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
6QFK_A	5.33e-112	187	1123	11	1030	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
2ZXQ_A	6.79e-112	187	1123	26	1045	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A9WNA0	2.54e-124	187	1123	51	965	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
Q2MGH6	1.48e-109	64	1123	188	1322	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	4.92e-109	64	1123	188	1322	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000919_01644.