CAZyme Information: MGYG000000902_01636

Basic Information

• Species: Clostridium neonatale
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium neonatale
• CAZyme ID: MGYG000000902_01636
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
750	MGYG000000902_17|CGC3	83837.44	5.021

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000902	4770522	MAG	China	Asia

• Gene Location: Start: 68202; End: 70454 Strand: +

Full Sequence      

MNSVSDFKEEIDELLSKMTLKEKVGQLNQRLYGWEIYKKTKTGYELTDTFKEEVKRWGGI
GAIYGLFRADPWSKVNFENGIDKEDSFKVINMIQEYIKENTRMKIPAIFSEESPHGHQSL
DGEMFPCNIAIGSTWNLDLIKEVSECISDELSEKGANLALVSTLDLLRDPRWGRSEECFS
EDPYLCAKYTEAIVDGYQKNNFENNVGVVLKHLCAQGAAVGGHNSGAANIGERELREIFL
PPVESGARRGAIGFMAAYNEIDGIPCHVNKKLLNDILRHEYQFNGIVMADGCALDRLTIM
TRDKEKAGKLAIEAGVDISLWDDIYTNLHRAVENKIIDELYIDDAVRRVLYVKFKLGLFD
KKKRNKKSYIDPSIINKKAASESIVLLKNNGVLPLSKEKKKIAVVGPNADTPYNQLGDYT
SPQRKNAVSTVLDGIKKFESNNITVNYSKGCSIRDISRSGFEEAINLAKNSDYTIVAIGG
SSARDFDLKFENNGAALIDDTKKDVNCGENVDLANLDLDGLQVELVRELSKTGTKVIVVL
IQGRPHSIENILDSSDAVLCAWYPGKMGGEAIAETIFGINNPNGKLAVSIPVSSMQLPCY
YNAKDSGAKIDYLDMSGLSLFPFGFGLSYTNFVYDKLRVDNEVTIEDLLKGKEVSIHVNI
KNCGDFDGYEVAQLYIRDEESSITRRVKELKGFKKVFIKSGEEVDVEFKLGFKELSIWDY
NLENRVEPGEVSIMVGKNSIDYIEKGLFLI

Enzyme Prediction     

EC	3.2.1.21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	98	320	1.7e-57	0.9861111111111112

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	4.03e-143	11	739	38	754	beta-glucosidase BglX.
COG1472	BglX	2.62e-78	18	445	1	397	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PLN03080	PLN03080	3.10e-61	125	708	114	740	Probable beta-xylosidase; Provisional
pfam00933	Glyco_hydro_3	4.20e-61	19	350	1	314	Glycosyl hydrolase family 3 N terminal domain.
pfam01915	Glyco_hydro_3_C	1.63e-56	384	629	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNF27516.1	4.42e-298	9	749	13	762
QGH37098.1	4.72e-291	18	749	1	738
QFK71465.1	3.87e-283	11	747	18	760
SQI53794.1	6.52e-277	9	741	12	753
QJD87999.1	6.47e-270	18	739	1	744

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XXL_A	7.71e-111	3	741	6	743	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	4.16e-110	3	741	6	743	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
5Z87_A	7.61e-110	59	750	88	785	ChainA, EmGH1 [Aurantiacibacter marinus],5Z87_B Chain B, EmGH1 [Aurantiacibacter marinus]
5TF0_A	7.69e-107	11	739	13	740	CrystalStructure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393],5TF0_B Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393]
6R5I_A	3.40e-101	11	739	7	722	ChainA, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5I_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KMH0	3.79e-118	73	739	25	710	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
T2KMH9	3.39e-106	2	742	31	747	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
P33363	4.85e-103	11	748	38	762	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
Q56078	4.85e-103	11	739	38	754	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
A7LXU3	9.70e-75	11	743	39	769	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000057	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000902_01636.