CAZyme Information: MGYG000000853_00718

Basic Information

• Species: Prevotella sp900549175
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900549175
• CAZyme ID: MGYG000000853_00718
• CAZy Family: PL11
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1520		162601.07	5.113

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000853	3157276	MAG	China	Asia

• Gene Location: Start: 23082; End: 27644 Strand: -

Full Sequence      

MKKKLLLLLFLGMTAVGGFAQRTTDAVDRGLVAVKTGSGVYCSWRITADEYYDVTYNIYR
DGTKINTEPLSVSNFTDRSGTVTSKYTVEAVVRGKAQAKSKEATPWARNYKEVKMNHGAL
TSTYIPNDACVADVDGDGELEILLKFDNASDAANGYKPAGNNGEYAIIEVYKMDGTRLWW
IDLGPNMADFQNNENNIVAYDWDGDGRAEAVMRAADGTTIHTATGQTIVIGNKSKNYRPS
GGMSGQWFIHEGSEFLLYLNGQTGEVYQQMEYPLKRLEAGETDLSKAWGDGYGHRSTKHF
FGAPYLDGRKPSIFLARGIYTRHKMIAFDVDPATHKLTERWRWNCNTGGAWYGQGYHNFG
IADVDWDGRDEIVFGSMVIDDNGKGLSTTGLGHGDSQHCSDFDPYSHGQEIFACNEDSPN
NNFRDATTSKIYYRTTGGNDDGRAIMGNFCNEYPGAQGSSTRDGSLISSVTHAAIPGGSK
ANIAQNFRIYWDGDLCSETFNYANGKNTAGTIYKAGRGAIATLEGSMTNNDTKGTPCYQG
DILGDWREEVIMRTADNNIRIYTTNVSTKWRNYTLWHDHQYRQAMVWQMCGYNQTPHTSY
FLGELEGITKAPAPLTMTGRTEIADGATIGSTENDKHIIMCETGDMTVGVADGAAPYILT
VNAPTWVQGHDNNNNITTETFTHTITGGAFTGSMRLVKQGDGVLNLPAVVQTYTGPTDVW
AGTLNFDGTLQSSRLWLNRFAVLNSAGTFAKGIEMEYASVLRPGGKDAKGVITTDSLILN
YGAMVELDLYGGSFEADKINMNTLKIDKKNWSNGPAYLSPVFKIVRHPAAGEDKLTPGRY
HIADVKAVDGNVDDIVVEGLAGVKAVVVYEDGKLYVEVQDMRDAMEIIWTGENGSVWDFA
ETSNFKTHGGSEEVFVDGDVVLFNDDAKTGEVVLAQNLTPSSVVFNNNELEYNITGKAIS
GGATLIKNGAAGTTISNANEYTGGTVIDGGKLTVGSLANDEGTDFGALGGADAAITINNG
ASLSVTGDVKTVQTINVGEGGATFDVPQNATLNLAAPVKSAVKAPVYKRGAGTLRISKGN
SFGKMYLEQGTIASLTANSASDKYFPDTLVFNGNNVTLNHDNTEYSMTENIHDYTNFSVP
AGKSGNIYLDGRCVYHGGLAGEGTFRVYPRYVRSYLSGDWSKFTGTLQICYNGTNSYGVN
AVTLQENSGARFPNAKVVVDDNAILDNNGVSLSLASVSGKGTLSGTGTYTIGSDGSDQNF
TVKFTSPVKKEGEGMWTIGTSNAQSSIGNVTINGGTLYLATFNSSTSVIGGSVTVNSGAS
VIGLGTVKSMTLNAGATLTPGQISATDPTGLFAVTSTLNIKSGAVVNLNVKSASNTNYSR
TYLKSDQYLMLSGTVNVTLSDAYTPKLGDEIILWQAKSFTGKPTVNLPALPDGLGWDTTG
LLAPTGVLRVVTFADGIGAVSGNAEVRCGVYTLSGLQVAGFVSTESEAAAHAGTLGLVSG
TYILRVNGQGVAKSVKLVIK

Enzyme Prediction     

EC	4.2.2.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL11	24	605	5.9e-164	0.9759862778730704

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd10318	RGL11	1.00e-177	28	600	5	564	Rhamnogalacturonan lyase of the polysaccharide lyase family 11. The rhamnogalacturonan lyase of the polysaccharide lyase family 11 (RGL11) cleaves glycoside bonds in polygalacturonan as well as RG (rhamnogalacturonan) type-I through a beta-elimination reaction. Functionally characterized members of this family, YesW and YesX from Bacillus subtilis, cleave glycoside bonds between rhamnose and galacturonic acid residues in the RG-I region of plant cell wall pectin. YesW and YesX work synergistically, with YesW cleaving the glycoside bond of the RG chain endolytically, and YesX converting the resultant oligosaccharides through an exotype reaction. This domain is sometimes found in architectures with non-catalytic carbohydrate-binding modules (CBMs). There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain through a beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
pfam18370	RGI_lyase	2.79e-36	22	106	1	86	Rhamnogalacturonan I lyases beta-sheet domain. This is the beta-sheet domain found in rhamnogalacturonan (RG) lyases, which are responsible for an initial cleavage of the RG type I (RG-I) region of plant cell wall pectin. Polysaccharide lyase family 11 carrying this domain, such as YesW (EC:4.2.2.23) and YesX (EC:4.2.2.24), cleave glycoside bonds between rhamnose and galacturonic acid residues in RG-I through a beta-elimination reaction. Other family members carrying this domain are hemagglutinin A, lysine gingipain (Kgp) and Chitinase C (EC:3.2.1.14).
PRK15319	PRK15319	1.57e-10	910	1406	694	1132	fibronectin-binding autotransporter adhesin ShdA.
PRK15316	PRK15316	0.001	964	1058	1881	1966	RatA-like protein; Provisional
PRK15319	PRK15319	0.001	961	1377	621	1044	fibronectin-binding autotransporter adhesin ShdA.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIM09959.1	0.0	15	1506	5	1528
AGB29439.1	0.0	1	1511	1	1514
BCS86257.1	6.89e-306	24	878	2	866
QZN78711.1	6.77e-132	25	614	2	574
APO48084.1	5.60e-131	13	614	16	600

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2Z8R_A	3.21e-129	20	614	1	578	Crystalstructure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8R_B Crystal structure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8S_A Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2Z8S_B Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2ZUX_A Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis],2ZUX_B Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis]
4CAG_A	4.19e-121	12	604	2	577	Bacilluslicheniformis Rhamnogalacturonan Lyase PL11 [Bacillus licheniformis]
2ZUY_A	1.63e-120	21	614	5	599	Crystalstructure of exotype rhamnogalacturonan lyase YesX [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31526	4.24e-128	20	614	38	615	Rhamnogalacturonan endolyase YesW OS=Bacillus subtilis (strain 168) OX=224308 GN=yesW PE=1 SV=1
O31527	7.06e-120	21	614	5	599	Rhamnogalacturonan exolyase YesX OS=Bacillus subtilis (strain 168) OX=224308 GN=yesX PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001002	0.989606	0.008634	0.000286	0.000241	0.000215

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000853_00718.