CAZyme Information: MGYG000000746_01391

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; UBA1777
• CAZyme ID: MGYG000000746_01391
• CAZy Family: GT39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
602		67913.05	7.8533

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000746	2046986	MAG	Kazakhstan	Asia

• Gene Location: Start: 47394; End: 49202 Strand: -

Full Sequence      

MNTILLLPLSLFFIIAALITLLPDLRRCDLSRRDNGRVTAGEVCVLALICAVYAAVAFFR
LGNTVSPESFASFKEGESVTIELPGEEHVASMSLFSGLNVGEFSIEFSVDGQEYFHASDF
DQNYVSIFKWNSISPDSSSLSAPARYVRISCTGGEPYLGEVAFFAEGRRLITPVGGGALT
DEQSTAPVESTFMDSSYFDEIYHARTAAEHIANIKPYEISHPPLGKLIIALGIRLFGLTP
FGWRFSGTLFGVLMLPALYFFCRSMFEKRVAACCTVIFAFDFMHFVQTRLATIDTYAVFF
IILMYWFMYLYVTQDKDGGEKRARTYLALSGVFFGLGAASKWTALYAGAGLAVIWLAHWI
IKLASSKESADARRRVRNGFFRNCGFCTVFFVVIPALIYYLSYFRYGTAAGLRAPGMFFT
KEYLDIVLDNQRFMFTYHVGIKATHPYASRWYQWVFDIKPILYFLHYGKDGIQQSFGAFV
NPALCWGGIISVVILCAVAIIRRDRTAAFICAGYFAQLVPWMFIDRLTFEYHYFPCTVFL
VLALGYIFELIARSERRWRLPVYGYTAVCTALFALFYPVLSGAPVYGLVVEKLLGWFPTW
PF

Enzyme Prediction     

No EC number prediction in MGYG000000746_01391.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	194	404	5.4e-55	0.905829596412556

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam16192	PMT_4TMC	4.77e-20	431	577	7	172	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
COG1928	PMT1	6.52e-17	198	403	50	264	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
COG1928	PMT1	4.25e-16	431	602	505	695	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
pfam13231	PMT_2	2.48e-15	220	358	1	132	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	1.69e-14	195	399	20	233	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANU42918.1	1.87e-158	27	602	57	653
QQR07704.1	1.87e-158	27	602	57	653
QIA32159.1	5.26e-157	27	602	53	649
QBB67038.1	7.58e-141	46	602	80	752
ALP95330.1	1.51e-140	46	602	80	752

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P9WN04	2.57e-22	198	600	73	521	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=pmt PE=3 SV=2
P9WN05	2.57e-22	198	600	73	521	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pmt PE=1 SV=2
L8F4Z2	1.40e-21	198	600	67	515	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycolicibacterium smegmatis (strain MKD8) OX=1214915 GN=pmt PE=3 SV=1
Q8NRZ6	5.86e-19	219	600	97	519	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) OX=196627 GN=pmt PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000048	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
4	23
36	58
240	262
269	286
290	312
325	339
344	366
379	401
478	500
507	524
529	551
558	580