CAZyme Information: MGYG000000745_01700

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74
• CAZyme ID: MGYG000000745_01700
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1125	MGYG000000745_39|CGC1	123167.9	6.0969

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000745	2615728	MAG	Kazakhstan	Asia

• Gene Location: Start: 19250; End: 22627 Strand: +

Full Sequence      

METLLNDGWEFVKTPLDATPEAARAAAWRKIGLPHDWLIGQHENLYEDSAGWYRRTLRAP
EPGRSLTLSFDGVYMDCRVLVNGRVAAAHAYGYTAFRVPLTPFLMPGENEIMVHARHQAP
NSRWYSGAGIFRDVTLLDLPARHLTPDGDYITARYDPVSGAGRLTLRCRAENTPDGETLL
VRLLDQQFNVLAQSAVPVANGEACWEYTAAHVSPWSPDAPSLYALEMSLAGETTRRRIGF
RTVETTAEGGLLLNGRHIKLHGVCLHHDLGALGAAFHADAARRQLRLMREMGVNALRTAH
NPPARQVMDLCDEMGLLVVNEAFDMWRKAKTPYDYARFFAACEAEDVESWVLRDRNHPSL
LMWSIGNEILDTHADAEAGYETARLLRDQVRALDPAGNGLITFGSNYMPWENTQRCAGLL
QAVGYNYGEKYYAAHHAAHPEWRIYGSETASLASSRGVYHFPIDAAILSDEDLQCSALGN
SATSWGARNLPALTDDERTPFSLGQFLWSGIDYIGEPTPYHTRSSYLGQADTACFPKDAY
YFFQALWTRAPMVHIGVYWDWNAGQLIDVPVMTNGDEAELFLNGVSLGRKAVSFGARESC
LPVWRVPYAPGELTAVAYRQGRPVARDAQRSFGESARIVLTASGDALPADGRSLAFITVG
MADESGAPVRNACDRVAVSVTGAGRLLGLDNGDSTDEEGYQTHTRRLFNGSLLAIIGAAD
TPGNIAVSVSSPGKAAQKIMLRALPVPRLPGVGCREGVAPGDCPPDRPVRRIRLTALGER
RLSPACPSVAFRALCLPENADGQKIEYRLVTAGGLDSPCGRLEETADGVIVHAVGDGAAY
LRATCKNGAAHARIISQLEINVTGFGAPYLDPYGFVPGGLHSLSSGDLGPGNEGGVAFAR
DGESMAGFERVDFGVAGSDEITLPIFALTGEEYCIALWLGVPDAGGRLLTRLRYRKPTIW
NTYQPETYRLPERLTGVQTLCFVLDRKLHLRGFSFTRQSRAWRSVAAGEADALYGDSFTR
AGDAVTNVGNNVTLLFRDMDFERATCARLHIDGETPLSSQPITLCQQAADGAESRRMLRF
DGGARHEQTFCVDILPNARQVSFLFLPGSRFNFYAFRFERVENES

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	544	1.6e-104	0.601063829787234

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	9.32e-59	1	369	11	407	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	3.29e-36	52	395	68	441	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.70e-34	4	368	43	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam18565	Glyco_hydro2_C5	1.95e-32	638	739	1	102	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK09525	lacZ	1.39e-27	47	368	118	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTE68512.1	0.0	1	1120	1	1124
QTE71175.1	0.0	1	1120	1	1119
QUC68071.1	0.0	1	1120	1	1118
QTE75139.1	0.0	1	1120	1	1119
AGA58497.1	0.0	4	1120	6	1141

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	3.75e-151	6	741	7	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	5.75e-147	6	733	13	797	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	1.12e-131	6	738	31	815	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4CU6_A	3.77e-124	6	731	19	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	1.02e-123	6	731	19	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	3.12e-134	6	733	48	838	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.49e-82	1	727	47	804	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	9.57e-47	5	617	56	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	2.22e-34	31	688	35	695	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
Q59140	1.08e-32	5	368	32	434	Beta-galactosidase OS=Arthrobacter sp. (strain B7) OX=86041 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000065	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000745_01700.