CAZyme Information: MGYG000000732_00816

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; TANB77; CAG-508; CAG-245
• CAZyme ID: MGYG000000732_00816
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
237		27073.05	6.2658

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000732	1710353	MAG	Kazakhstan	Asia

• Gene Location: Start: 58615; End: 59328 Strand: -

Full Sequence      

MKFIRRLLLFLLIIIVIVCSVFYMNGKKLYDSKLSSISLEDKISSIKSDESFVSLKDLPD
YYKNAVIAVEDHRYYNHGAIDPIALARAIVSNIKQKEFKEGGSTITQQTAKNLYFISEDD
VVSRKVAEGILSFRLEKDYSKDDILELYVNTIYFGNGYYGIKEACEGYLKKEPKDMTLYD
ATMLAGIPNAPSVYAPTVNFNLTQSRQHKVVSSMLEYKYITQEQSDALNEEITNAKW

Enzyme Prediction     

No EC number prediction in MGYG000000732_00816.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	47	214	6.7e-57	0.9378531073446328

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00912	Transgly	1.43e-70	53	215	16	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
COG0744	MrcB	6.07e-69	28	233	57	260	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	8.89e-63	52	233	69	250	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	1.00e-42	53	237	69	252	penicillin-binding protein 1a; Provisional
PRK13481	PRK13481	2.47e-37	34	224	29	224	glycosyltransferase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRP41835.1	7.07e-71	24	225	27	227
ASN93505.1	7.07e-71	24	225	27	227
QJU22158.1	1.42e-70	24	225	27	227
QIX92252.1	2.01e-70	24	225	27	227
ANU46909.1	4.04e-70	24	225	27	227

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3NB6_A	7.92e-41	52	230	22	200	Crystalstructure of Aquifex aeolicus peptidoglycan glycosyltransferase in complex with Methylphosphoryl Neryl Moenomycin [Aquifex aeolicus]
2OQO_A	1.12e-40	52	230	22	200	Crystalstructure of a peptidoglycan glycosyltransferase from a class A PBP: insight into bacterial cell wall synthesis [Aquifex aeolicus VF5],3D3H_A Crystal structure of a complex of the peptidoglycan glycosyltransferase domain from Aquifex aeolicus and neryl moenomycin A [Aquifex aeolicus],3NB7_A Crystal structure of Aquifex Aeolicus Peptidoglycan Glycosyltransferase in complex with Decarboxylated Neryl Moenomycin [Aquifex aeolicus]
4OON_A	2.74e-36	53	237	42	225	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3DWK_A	1.87e-34	53	224	30	200	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
5U2G_A	2.91e-34	53	231	43	220	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O87579	1.23e-44	34	232	50	250	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
O86088	2.58e-42	34	232	50	250	Penicillin-binding protein 1A OS=Neisseria cinerea OX=483 GN=mrcA PE=3 SV=1
Q5FAC7	2.58e-42	52	232	70	250	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=mrcA PE=3 SV=3
P0A0Z5	2.58e-42	52	232	70	250	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=mrcA PE=3 SV=1
P0A0Z6	2.58e-42	52	232	70	250	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.796905	0.041466	0.159491	0.000207	0.000116	0.001828

TMHMM  Annotations     

start	end
7	24