CAZyme Information: MGYG000000701_00865

Basic Information

• Species: Prevotella sp900552675
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900552675
• CAZyme ID: MGYG000000701_00865
• CAZy Family: GH76
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1280	MGYG000000701_11|CGC1	140319.92	6.4631

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000701	3061545	MAG	Kazakhstan	Asia

• Gene Location: Start: 48344; End: 52186 Strand: +

Full Sequence      

MKKLILTVACIAVVQGLWADVEAGKAYRLVPATDKGKAVFVENSAFDNGKKVVLWTQTPA
PSQQWYAERQGEKWVLRNVYTGKYLTIASTVAQQSDKATATSAQWTLEPINASTNTYRVA
QTIGTQLRYLGAVKTSDGTQLALGVKKTGDDAAQQSWTFVEETPITTFTHALRDEMGEHY
LASFLQTVSGGKTFTKGGWGEAEILETMLDAYETTGQKQYLDAFTSVYNYFKKKVGTNWL
HLVYEDAYKWYGHDFNDDVMWMIIAAARAYQLTGQKVYINDAKRAFDGIWQRAYNQWGML
RWAEQPGTGNKNGTNSCINGPAEVAACYIAMGLGDESYYEKARALYDKQRRYLFNAETGA
VYDCFTWNAETNLPGGYSYWSSTYNQGTMLGAATLLYNHYGDNFYLQDAQKIVDYSLNHL
CNANGIINVCQTVEGDLCGFKGILMRYMRQYATRFNVPSIMTWMQRNALHAYSNRNSKGI
TSSAWLTKAAEDGTYGDKRYDDQPFGCSTAVSAAFNAFVPTSGIVKEAWQVFQAEDFDYA
QGVYTDQIDGRTVVTNIKKGLYTCYRNVDFGSTPARQVQLTLSKVANKAVGGSVEVCLDS
PSAKPIATINAPTTDSWATLTADIAPVAGRHDVYMRFKTKVTSSNSAFSFDSFTFLTTAT
PMMSDVTKNGGVLTLSPASNQAALAIDADPTTSASVAGHQLSLSYQSEVAVRPLQYSVSV
GTEPSANDPKTWTLMASNDGETWIELDRQDNAQFADRASTQRHTLNTDVLYTRFRLNITD
NHGGKNTAVSDWQLVGTLNTNLGFTAQGTEVCMADGLYKPTRYAVTAPATTGQQAPTGWT
LQGSTDGKTWDDIDHRSHQSFACAAATAFYTASAQKGYSQFRLLTEGEGTAQTYTVFAQL
MPTATFYPGIFSNGGTFTAASSTASLKAIADADPSTSVRIPLTNGQAQVVYESPWPFKVK
SYALCAGSAQGETQANPKNWKVEVSKDGTKWTVLRTELSKAFNKRATVVTNTLSSNAEWK
YIRLNITAVSKADADEVVIDDWQLNGQCVTTDALNASGATTTALVPVDNANETGDKVADN
NIDTKYCYPFFMGSWLNYNFDTPVVANLYSVSSANDNAGRDPKAWTLMGSDNGTDWVTLD
QQTQIAFPNRKTTLFFAFNNKHAYAHYRLVMDGNNGDSMLQLSEWQLLYSDQIASGIGAV
ADHVSQAKIGINKDVENKLLTLSMPTNGTVAVYNSTGLLAMQQTVAAGKQQVALPSHARG
IYIIKVCAGAQQQTAKIMIR

Enzyme Prediction     

No EC number prediction in MGYG000000701_00865.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH76	176	488	5.7e-52	0.8463687150837989
CBM6	533	655	3.8e-23	0.855072463768116

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam03663	Glyco_hydro_76	4.55e-36	199	496	36	340	Glycosyl hydrolase family 76. Family of alpha-1,6-mannanases.
cd04084	CBM6_xylanase-like	4.06e-27	533	655	4	123	Carbohydrate Binding Module 6 (CBM6); many are appended to glycoside hydrolase (GH) family 11 and GH43 xylanase domains. This family includes carbohydrate binding module 6 (CBM6) domains that are appended mainly to glycoside hydrolase (GH) family domains, including GH3, GH11, and GH43 domains. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB6s belonging to this family are Microbispora bispora GghA, a 1,4-beta-D-glucan glucohydrolase (GH3); Clostridium thermocellum xylanase U (GH11), and Penicillium purpurogenum ABF3, a bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (GH43). GH3 comprises enzymes with activities including beta-glucosidase (hydrolyzes beta-galactosidase) and beta-xylosidase (hydrolyzes 1,4-beta-D-xylosidase). GH11 family comprises enzymes with xylanase (endo-1,4-beta-xylanase) activity which catalyze the hydrolysis of beta-1,4 bonds of xylan, the major component of hemicelluloses, to generate xylooligosaccharides and xylose. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold.
pfam03422	CBM_6	1.21e-17	533	655	1	123	Carbohydrate binding module (family 6).
smart00606	CBD_IV	4.64e-17	533	655	9	129	Cellulose Binding Domain Type IV.
COG4833	COG4833	7.05e-14	199	454	46	280	Predicted alpha-1,6-mannanase, GH76 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT74831.1	1.63e-189	1	1266	1	1297
AYD48939.1	3.68e-70	4	517	8	495
QIA07414.1	3.68e-70	130	638	178	658
QEC70572.1	2.53e-66	20	516	27	494
QOV19890.1	1.40e-61	197	656	57	493

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5M77_A	1.40e-36	199	665	73	531	ChainA, Alpha-1,6-mannanase [Niallia circulans],5M77_B Chain B, Alpha-1,6-mannanase [Niallia circulans]
4BOK_A	2.24e-29	199	495	39	326	ChainA, Alpha-1,6-mannanase [Niallia circulans]
4BOJ_A	2.38e-29	199	495	42	329	ChainA, Alpha-1,6-mannanase [Niallia circulans],4BOJ_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4BOJ_C Chain C, Alpha-1,6-mannanase [Niallia circulans]
4D4A_A	1.12e-28	199	495	60	347	ChainA, Alpha-1,6-mannanase [Niallia circulans],4D4A_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4D4B_A Chain A, Alpha-1,6-mannanase [Niallia circulans],4D4B_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4D4C_A Chain A, Alpha-1,6-mannanase [Niallia circulans],4D4C_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4D4D_A Chain A, Alpha-1,6-mannanase [Niallia circulans],4D4D_B Chain B, Alpha-1,6-mannanase [Niallia circulans],5N0F_A Chain A, Alpha-1,6-mannanase [Niallia circulans],5N0F_B Chain B, Alpha-1,6-mannanase [Niallia circulans],6ZBX_A Chain A, Alpha-1,6-mannanase [Niallia circulans],6ZBX_B Chain B, Alpha-1,6-mannanase [Niallia circulans],7NL5_A Chain A, Alpha-1,6-mannanase [Niallia circulans]
5AGD_A	5.02e-28	199	495	60	347	ChainA, Alpha-1,6-mannanase [Niallia circulans],5AGD_B Chain B, Alpha-1,6-mannanase [Niallia circulans],6ZBM_A Chain A, Alpha-1,6-mannanase [Niallia circulans],6ZBW_A Chain A, Alpha-1,6-mannanase [Niallia circulans],6ZBW_B Chain B, Alpha-1,6-mannanase [Niallia circulans]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000271	0.999036	0.000201	0.000164	0.000152	0.000143

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000701_00865.