CAZyme Information: MGYG000000647_02003

Basic Information

• Species: CAG-390 sp900753295
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-272; CAG-390; CAG-390 sp900753295
• CAZyme ID: MGYG000000647_02003
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
592	MGYG000000647_93|CGC1	67446.35	4.729

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000647	2268104	MAG	Germany	Europe

• Gene Location: Start: 2696; End: 4474 Strand: +

Full Sequence      

MTVPRNEYPRPQLVRNEWLCLNGEWQFEADNAKSGMERDFYANDYTLNGKINVPFCPESR
LSGIGNRDFIASVWYKRAVDIPAEWRGKRIILHFGAVDYHAIVFVNGTRVCEHRGGYTPF
SADITEFLTWDGDMISLCAYDDVRSHDQPAGKQSAKYNSYGCFYTRTTGIWQTVWLEAVG
DCYINEIKFTPDVPSVSVSAGFVLEGDFRGAELSVRVSFDGREVGRADVAGITARTVNIA
IPLSECHLWDVGEGNLYDVSVEVRRDGAVLDSAESYFGLRSVSLDGRVLRLNGRVVFGRW
VLDQGFYPDGIYTAPTDDDLRGDIEKSMELGFNGARLHEKVFEPRFLYWADKLGYLCWGE
HANWGLNVSEAGQIQHFLPEWLEAVARDYSHPSVIGWCPFNETWDYAGRAQDNSVLSTVY
RVTKALDPTRPVIDTSGNYHVETDIFDVHDYEQNPELFRTYYDKIPEGIINDQIQRNAAR
RTRQKYDGKLPVFVSEYGGIGWMPEGKAGWGYGKGPATEEEFIARYRGLTDALLDNEYIL
GFCYTQLYDVEQEKNGLMTYDRKFKFDPAIFREINTRTAAIEKNDAETPEKK

Enzyme Prediction     

No EC number prediction in MGYG000000647_02003.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	14	454	2.1e-93	0.5132978723404256

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	5.02e-47	10	448	3	466	beta-D-glucuronidase; Provisional
COG3250	LacZ	6.89e-36	21	456	15	451	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	8.73e-16	21	444	44	477	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	3.44e-14	21	138	4	132	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
PRK09525	lacZ	5.17e-11	8	432	64	485	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANY74929.1	8.63e-255	3	582	7	581
ALS26546.1	2.03e-252	3	582	9	586
SMF87854.1	1.18e-247	2	582	6	581
AIQ16879.1	2.37e-247	2	582	6	581
AIQ73223.1	4.77e-247	2	582	6	581

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	5.00e-90	7	577	28	581	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
3K4A_A	1.34e-39	21	498	17	508	Crystalstructure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12],3K4A_B Crystal structure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12]
4JHZ_A	4.49e-39	21	498	17	508	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12],4JHZ_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12]
3LPF_A	4.58e-39	21	498	17	508	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPF_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPG_A Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],3LPG_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],5CZK_A Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12],5CZK_B Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12]
6LEM_B	8.12e-39	21	498	13	504	ChainB, Beta-D-glucuronidase [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	4.54e-38	21	498	15	506	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
O97524	7.76e-34	8	498	29	541	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1
O18835	3.43e-33	8	498	29	541	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1
Q4FAT7	1.20e-31	8	498	30	542	Beta-glucuronidase OS=Sus scrofa OX=9823 GN=GUSB PE=3 SV=1
P12265	4.11e-29	17	498	37	538	Beta-glucuronidase OS=Mus musculus OX=10090 GN=Gusb PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000006	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000647_02003.