CAZyme Information: MGYG000000488_00797

Basic Information

• Species: Anaeroplasma sp900767915
• Lineage: Bacteria; Firmicutes; Bacilli; Acholeplasmatales; Anaeroplasmataceae; Anaeroplasma; Anaeroplasma sp900767915
• CAZyme ID: MGYG000000488_00797
• CAZy Family: GT8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
279		33388.9	8.7564

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000488	1945837	MAG	Fiji	Oceania

• Gene Location: Start: 17726; End: 18565 Strand: +

Full Sequence      

MINVLYCGNDKVFDGILSSMISILKRTKTEEPFHFYIFTMDATFVNKKYLPISDKMYHFI
DLLAKSYNKDNLVSLIDVGEYYNKEFRNCPNEECYCSPYTLLRLFLDEIPNMPKDKVLYL
DVDLLFNKDITLLYNIDITGYEYSASKDQYGKYLVHPNYINAGVLLFNMEEIKKTKLLTK
ARELIKIKHLPFADQSAILRSTTKRKMLPQCFNDQRGVNKKTVVRHFAQKLYWLPYPHIE
NIKQWNLDRYFKVFNYKELNDIIYDFIYMKECFLRNKEI

Enzyme Prediction     

No EC number prediction in MGYG000000488_00797.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	3	229	3.2e-30	0.8443579766536965

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04194	GT8_A4GalT_like	1.83e-26	2	198	1	192	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
pfam01501	Glyco_transf_8	4.19e-15	3	195	1	197	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
cd00505	Glyco_transf_8	1.32e-11	4	183	4	176	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
COG1442	RfaJ	3.96e-11	2	197	3	194	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].
cd06429	GT8_like_1	2.38e-08	21	176	18	166	GT8_like_1 represents a subfamily of GT8 with unknown function. A subfamily of glycosyltransferase family 8 with unknown function: Glycosyltransferase family 8 comprises enzymes with a number of known activities; lipopolysaccharide galactosyltransferase lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase and inositol 1-alpha-galactosyltransferase. It is classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNL97925.1	3.24e-109	1	276	1	275
QQA01162.1	1.55e-108	1	279	1	276
AEE15925.1	2.19e-59	1	267	1	264
QAT42778.1	5.81e-53	3	262	2	262
AFR21129.1	1.07e-49	3	271	2	274

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000055	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000488_00797.