CAZyme Information: MGYG000000424_00347

Basic Information

• Species: CAG-353 sp900768995
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; CAG-353; CAG-353 sp900768995
• CAZyme ID: MGYG000000424_00347
• CAZy Family: GH94
• CAZyme Description: N,N'-diacetylchitobiose phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
799	MGYG000000424_2|CGC1	90123.3	5.6588

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000424	2854163	MAG	Sweden	Europe

• Gene Location: Start: 97515; End: 99914 Strand: +

Full Sequence      

MNYGHFDLKNKEYVITRPDTPMPWANYLGDPEYGAIISNNAGGYSFVKSGANGRIIRYRF
NSVAVDQPGRYIYLKDGETGDYWSASWSPVCKPLDEYKSECRHGTAYTVITSEYKNIKTE
TTYYVPRNALYEVWASKITNNDTKPRKLSVTGYCEFVNDNNYEQDQVNLQYTLFITRTYF
KGNFILQKQNEIFKNPNNERFLGVAKADVSAYCGDRDSFVGAYRGYANPKGIEEGLKGEL
NYNTNSCGALQSDIVLQPGETKEIIYVLGQRPEAVAAEIIAKYQKDGVVEAELAELKEFW
HTKLGNFEVDTPDADFNNMVNVWNAYNCFITFIWSRAASFQYCGLRNGFGYRDTVQDIQG
IIHLAPEMAKKQIVFMLSAQVTNGAGLPLVKYDHKAGQENHPDENDPNTAYAKETGHPSY
RADDALWLFPTIYKYISESGDVSFLDEEILYANDGEKGTVYDHLKRAIMFSMNNLGNHGM
PAGLHADWNDCLRLGKKGESTFVAFQLVYAVKILRSYAELKNDAEYIKYLDEVKAKLDTI
LAACWNEDRWIRGYKEDGAVIGQRTDPEANMWLNPQSWSVISGFASKEQAEKAMDSVERE
LNTPYGAMVMYPPYVEHGFDGALMQCFNKCTKENAGIFSQPQGWLILAESLMGHGNRAYK
YWKEAAPATYNDNAEHRCLEPYVYGQFVEGKDSPYAGRAHVHWLTGTASTVMVGTVEGIL
GMRPDAAGLKVDPSISSEWGSYKINKTFRGKKLHMTFNNPDHVESGVKEIVLNGEKLPSN
HIPADKLKDVNEIVVTLGK

Enzyme Prediction     

EC	2.4.1.49

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	2	795	2.4e-258	0.7461389961389961

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	0.0	1	798	271	1054	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
cd11755	GH94N_ChBP_like	1.48e-171	1	295	1	300	N-terminal domain of chitobiose phosphorylase (ChBP) and similar proteins. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes chitobiose phosphorylase (EC:2.4.1.-). This N-terminal domain is involved in oligomerization and may play a role in catalysis, but it is separate from the catalytic domain [an (alpha/alpha)(6) barrel]. Chitobiose phosphorylase catalyzes the reversible phosphate dependent hydrolysis of chitobiose [(GlcNAc)2] into alpha-GlcNAc-1-phosphate and GlcNAc. In some organisms, ChBP may be involved in the production of GlcNac-6-phosphate in intracellular pathways.
pfam17167	Glyco_hydro_36	2.69e-162	299	723	1	425	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.
pfam06165	Glyco_transf_36	1.11e-98	21	269	1	247	Glycosyltransferase family 36. The glycosyltransferase family 36 includes cellobiose phosphorylase (EC:2.4.1.20), cellodextrin phosphorylase (EC:2.4.1.49), chitobiose phosphorylase (EC:2.4.1.-). Many members of this family contain two copies of this domain.
cd11754	GH94N_CBP_like	8.29e-81	1	295	1	303	N-terminal domain of cellobiose phosphorylase (CBP) and similar proteins. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cellobiose phosphorylase (EC:2.4.1.20) or cellobiose:phosphate alpha-D-glucosyltransferase, or CepA. This N-terminal domain is involved in oligomerization and may play a role in catalysis, but it is separate from the catalytic domain [an (alpha/alpha)(6) barrel]. Cellobiose phosphorylase participates in the degradation of cellulose, it catalyzes the phosphate dependent hydrolysis of cellobiose into alpha-D-glucose-1-phosphate and D-glucose, a reversible reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADD61963.1	0.0	1	798	1	802
CBL18243.1	0.0	1	798	1	798
BCJ96219.1	0.0	1	797	1	797
QNM00846.1	0.0	1	797	1	800
CCO03814.1	0.0	1	798	1	802

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1V7V_A	1.57e-170	1	794	1	797	Crystalstructure of Vibrio proteolyticus chitobiose phosphorylase [Vibrio proteolyticus],1V7W_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc [Vibrio proteolyticus],1V7X_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate [Vibrio proteolyticus]
3QDE_A	6.80e-160	1	798	1	811	ChainA, Cellobiose phosphorylase [Acetivibrio thermocellus],3QDE_B Chain B, Cellobiose phosphorylase [Acetivibrio thermocellus]
3AFJ_A	4.83e-147	1	759	21	805	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127],3AFJ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127]
2CQS_A	6.80e-147	1	759	21	805	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQS_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQT_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],2CQT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],3QFY_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFY_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFZ_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QFZ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus]
3ACT_A	6.80e-147	1	759	21	805	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant [Cellulomonas gilvus ATCC 13127],3ACT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant [Cellulomonas gilvus ATCC 13127]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q76IQ9	4.14e-172	1	798	1	801	N,N'-diacetylchitobiose phosphorylase OS=Vibrio proteolyticus OX=671 GN=chbP PE=1 SV=1
Q9F8X1	3.17e-171	1	798	1	800	N,N'-diacetylchitobiose phosphorylase OS=Vibrio furnissii OX=29494 GN=chbP PE=1 SV=1
B9K7M6	3.75e-163	1	798	1	813	Cellobiose phosphorylase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=cbpA PE=1 SV=1
P20471	8.25e-71	3	750	2052	2798	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2
Q7S0S2	2.52e-68	68	790	83	778	Cellobionic acid phosphorylase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=NCU09425 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000061	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000424_00347.