CAZyme Information: MGYG000000289_01161

Basic Information

• Species: Prevotella buccae
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella buccae
• CAZyme ID: MGYG000000289_01161
• CAZy Family: GH128
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1513	MGYG000000289_13|CGC1	167053.35	6.5833

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000289	3001000	MAG	Sweden	Europe

• Gene Location: Start: 35058; End: 39599 Strand: +

Full Sequence      

MRKAIPLLLLWGCLVANKAFAQQQPPFPKKNELPVTLLQNGRYNGREVSITKLTSPKPGS
TYTLEVKGKINSGTGRGLDISANDASGMGFRVAMDAASLNWNNPLATLSSLSSTDNTKLR
TLRFTVADKKVTVYEDGYFVATRPLETIKEAMNPDGPNINPEVIPVGDWGQNKPTPTSKG
WYLVNENKVMNEWPANVRFESSPKNTLYNSDGTQYHGNCLFIRWDANSYKTYNYAYEVEL
AANTTYRFSMDCAYWANGHASTITAKVSDSRTLNTTLASHVFKTAKERVLVPGEFTFTTP
HAGKYYISFSSTWALFAIANLQLVEAGLSAEPMIIIGKNYEGEADMEVAAVTYDESGVYA
PETTSGEPTVVNLDNAGDVSKGYLFNSDLHVSGTTNLHFTGRPAPFSNTTIDLKGEDAWL
YLDYVKPSKTIKECLPSVKINGAAAENGKNCRVSVWANGTVVIPNGQAYDHKALVAYDGE
NFTGKSKTFEINIYHNNLGEWDNKIRSFKLKKGYMATLANNANGTGYSRVFIADDADLEV
PVLPEGMEQFVSFVRVFRWNWVSKKGKANGYGEKNQLNITCNYDWNVGGMSTDPDIEYSP
IRQNLYWPGWNDINNKPGVTHLLGCNEPDRPDQSNATVDQIVEMWPEMMKGGYRVGSPAP
SSVWAWNGAFFNTIDSLNYRVDFAVAHIYESTGPETLVSRIKTLSDKGKGRPVWITEWNN
GANWTNEYWPTASGPRRDADMNIIYGEDGSTKTVNRPLSPENAEKQRAYMAAALPALDQC
NLIERYFEYDWVQDCRALMLGGKPTPAGKLYGEHKAALAYRKANAYNHVWKIAPPFPLMT
IDGEFRNITLKWYDHNGETGKKYILERKMDGEADFAPYKEFVLGQDYRAGETVTFTEAIP
CLSKVEYRIKALSYKDKESIYSRVKAFTRDAAVPAPTLRGEALSTSIIKLSWDAVKGVKA
YRIERAETETGDYRTVADNLTATTYQDEKLQPATNYYYRAYSLNTAAEHPASTVLAVSTK
ALTIPEPVTGLHISGGTGSVSLRWAFAYDAQYRVARATRADGEYTVVADKVEATSYVDTG
LTNGLTYYYKVQPYNGAGFGEESPVLSATPAAGRHLHLAFDEQEGHTAYDEWGGYDAELH
NGASFVEGRNGGRAVKLAKGSRSYMELPAGAVSRLSDFTIATWLKLPGGKGRVFDFGNGT
GTFMIGAANNEGLRYKITCAKGAFDITVPMKWSADEWNHLAITQNGTDVRFYLNGEEVGK
GTNASGIQPKDMGLTRQNWLGRSQWANDAYCDHIYDDFRIYDCALSETDVQALYVDREPA
IDPQYARTVRPGSWNTICLPHAAMPAEGVTAYNISGINEARSVLYLSPVATMTAGVPYIF
RAEGDLAVFRENGLPAEAPADGANGLRGVFESKAGDVADGAYILRNDVWYRVDNQAQFNL
GNNRAYLPSLDAVTQTLPTGAKKMSIDHTGTTGIDTPRSSAEATKAYTIDGIKVQKPHKG
VYIINGKKQVKGK

Enzyme Prediction     

No EC number prediction in MGYG000000289_01161.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH128	575	811	4.5e-27	0.9732142857142857

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam13385	Laminin_G_3	4.12e-24	1160	1311	2	151	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.
pfam11790	Glyco_hydro_cc	3.54e-17	614	811	59	235	Glycosyl hydrolase catalytic core. This family is probably a glycosyl hydrolase, and is conserved in fungi and some Proteobacteria. The pombe member is annotated as being from IPR013781.
cd00063	FN3	6.45e-07	1025	1109	1	92	Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.
smart00060	FN3	8.99e-07	1025	1100	1	83	Fibronectin type 3 domain. One of three types of internal repeat within the plasma protein, fibronectin. The tenth fibronectin type III repeat contains a RGD cell recognition sequence in a flexible loop between 2 strands. Type III modules are present in both extracellular and intracellular proteins.
COG3401	FN3	1.68e-05	907	1091	51	232	Fibronectin type 3 domain [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AXY72824.1	3.00e-300	1	1313	1	1301
AUC19783.1	3.87e-288	29	1310	3	1314
APZ45921.1	3.87e-288	29	1310	3	1314
AEV99551.1	3.01e-273	7	1313	19	1336
BAV05333.1	1.98e-177	201	948	364	1078

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6UAQ_A	1.92e-08	616	823	85	262	Crystalstructure of a GH128 (subgroup I) endo-beta-1,3-glucanase from Amycolatopsis mediterranei (AmGH128_I) [Amycolatopsis mediterranei],6UAR_A Crystal structure of a GH128 (subgroup I) endo-beta-1,3-glucanase from Amycolatopsis mediterranei (AmGH128_I) in complex with laminaritriose [Amycolatopsis mediterranei]
6UFL_A	4.63e-08	616	823	85	262	Crystalstructure of a GH128 (subgroup I) endo-beta-1,3-glucanase (E199Q mutant) from Amycolatopsis mediterranei (AmGH128_I) in the complex with laminarihexaose [Amycolatopsis mediterranei],6UFZ_A Crystal structure of a GH128 (subgroup I) endo-beta-1,3-glucanase (E199Q mutant) from Amycolatopsis mediterranei (AmGH128_I) [Amycolatopsis mediterranei]
6UAS_A	1.11e-07	616	823	85	262	Crystalstructure of a GH128 (subgroup I) endo-beta-1,3-glucanase (E199A mutant) from Amycolatopsis mediterranei (AmGH128_I) in complex with laminaripentaose [Amycolatopsis mediterranei]
6UAT_A	1.11e-07	616	823	85	262	Crystalstructure of a GH128 (subgroup I) endo-beta-1,3-glucanase (E102A mutant) from Amycolatopsis mediterranei (AmGH128_I) in complex with laminaripentaose [Amycolatopsis mediterranei],6UAU_A Crystal structure of a GH128 (subgroup I) endo-beta-1,3-glucanase (E102A mutant) from Amycolatopsis mediterranei (AmGH128_I) in complex with laminaritriose and laminaribiose [Amycolatopsis mediterranei]
3MPC_A	1.24e-06	944	1008	18	82	ChainA, Fn3-like protein [Acetivibrio thermocellus ATCC 27405],3MPC_B Chain B, Fn3-like protein [Acetivibrio thermocellus ATCC 27405]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P38939	1.55e-06	1022	1098	925	1002	Amylopullulanase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=apu PE=1 SV=2
P16950	3.47e-06	1022	1098	926	1003	Amylopullulanase OS=Thermoanaerobacter thermohydrosulfuricus OX=1516 GN=apu PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000568	0.923745	0.074714	0.000350	0.000332	0.000279

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000289_01161.