CAZyme Information: MGYG000000217_01640

Basic Information

• Species: Acetatifactor sp900066565
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Acetatifactor; Acetatifactor sp900066565
• CAZyme ID: MGYG000000217_01640
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase large subunit

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
639	MGYG000000217_9|CGC1	74312.22	4.7032

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000217	3653823	Isolate	China	Asia

• Gene Location: Start: 3649; End: 5568 Strand: +

Full Sequence      

MEADIRWLDDPQVFRVGQLPAHSDHPIYQDTEEAAEGRSSLVQSLDGTWEFAYSVNAKSR
PVDFYRENADSAQFDTIRVPGHIELAGYDQIHYINNMYPWEGKIYRRPAYTLGKGLVEGA
FSEAEYNPVGSYRKRFHLAEGLRGKRVIICFEGVEQAMYVWLNGHFVGYAEDSFTPSEFD
LTPYIKEKDNLLAVEVHKRSTAAFLEDQDFFRFFGIFRSVELYAKPQWHVEDLWAKPYFS
VEDGKGTLDAAIKISAEGEGQRPGKMRVCLSDANGKCLLEQTQILQSQVEQTLHLRETLA
EKVVPWSHENPYLYQLEITLTDSEGEVTEVVPYKIGFRDFILDPTDRVMKLNGERLVICG
VNRHEWNAASGRCISLEDMKWDIECFKRNHINAVRTCHYPDRMEWYTLCDEAGIYMMAET
NLESHGSWQKMGAVEPSWNVPGSLPEWKEAVVDRARTNFECFKNHPSILFWSLGNESYAG
DDIAAMQQFFKEKDDQRLVHYEGVFHNRAYEDRISDVESRMYAYPQEIIDYLEHDPKKPY
LLCEYMHDMGNSLGGMKSYMELLDRFEMYQGGFIWDYIDQALWVEDEVTGRRVLRYGGDF
DDRPSDYEFSGNGILFADRSEKPAMQEVRYYYGTQNRNR

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	28	632	1.1e-145	0.6263297872340425

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10340	ebgA	1.38e-158	6	629	3	596	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	2.56e-141	11	630	20	623	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	8.99e-131	345	633	4	298	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
COG3250	LacZ	2.51e-123	33	638	3	557	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02837	Glyco_hydro_2_N	1.24e-30	42	226	1	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM02889.1	0.0	1	639	1	639
ABX42393.1	2.14e-315	1	633	1	636
AQP40966.1	9.36e-308	1	633	1	630
QEH67710.1	9.20e-304	1	632	1	622
CBK94938.1	1.59e-301	1	633	1	630

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	6.13e-119	9	632	5	593	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	6.25e-119	9	632	6	594	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
3DEC_A	3.91e-105	16	632	15	613	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
3BGA_A	3.58e-101	38	632	44	617	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
1F4A_A	6.00e-101	9	630	15	620	E.COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_B E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_C E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_D E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4H_A E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_B E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_C E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_D E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q48846	1.30e-287	1	633	1	622	Beta-galactosidase large subunit OS=Latilactobacillus sakei OX=1599 GN=lacL PE=3 SV=1
Q02603	1.56e-286	1	633	1	623	Beta-galactosidase large subunit OS=Leuconostoc lactis OX=1246 GN=lacL PE=1 SV=1
O07684	1.08e-277	1	633	1	625	Beta-galactosidase large subunit OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=lacL PE=3 SV=2
Q7WTB4	2.05e-275	1	633	1	625	Beta-galactosidase large subunit OS=Lactobacillus helveticus OX=1587 GN=lacL PE=2 SV=1
P24131	1.92e-214	7	632	10	612	Beta-galactosidase OS=Clostridium acetobutylicum OX=1488 GN=cbgA PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000052	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000217_01640.