CAZyme Information: MGYG000000196_01563

Basic Information

• Species: Bacteroides thetaiotaomicron
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides thetaiotaomicron
• CAZyme ID: MGYG000000196_01563
• CAZy Family: GH78
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
729		84035.24	5.2321

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000196	6397474	Isolate	China	Asia

• Gene Location: Start: 238396; End: 240585 Strand: -

Full Sequence      

MILLGALSLASSTFAQTWIWYPGDYEIWLGNQMNNRRTERGAFFPPFWKTDSHYVVVEFS
KVLNLSEPEEVFIAAEGTYNVKLDGKLQFGMPETLLLPAGKHSLNIKVWNQATPPTIYVK
GKTVNSDSSWRVTYEDKEWIDESGKASDTSATIYMDAGCWNFDDATQRPSQFSLMREPQQ
PVAKTEQPEGGILYDFGKETFGFITLKNLSGKGKIDLYYGESPEEAKDKAYCETLDKLLL
EPGQITDLAIRSTSPLHHSDNEYTLENSKAFRYVYITHEPEVQIGEVSMQYEYLPEEYRG
NFRCNDEELNRIWEVGAYTMHLTTREFFIDGIKRDRWVWSGDAIQSYLMNYYLFFDSESV
KRTIWLLRGKDPVTSHSNTIMDYTFYWFLSVYDYYMYSGDRHFVNQLYPRMQTMMDYVLG
RTNKNGMVVGMSGDWVFVDWADGYLDKKGELSFEQVLFCRSLETMALCADLVGDKDGQQK
YEKLASALKAKLEPTFWNNQKQAFVHNCVDGRQSDAVTRYANMFSVFFDYLNADKQQAIK
QSVLLNDEILKITTPYMRFYELEAFCALGEQETVMKEMKAYWGGMLKAGATSFWEKYNPE
ESGTQHLAMYGRPYGKSLCHAWGASPIYLLGKYYLGVKPTKEGYKEFAVSPVLGGLKWME
GTVPTPNGDIHVYMDNKTIKVKATEGKGYLTIQSRRQPKANMGTVEKVSEGVWRLWIDSP
EERIVTYRL

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	186	680	2.2e-115	0.9642857142857143

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	8.06e-26	300	633	4	339	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	3.32e-05	636	672	1	38	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
COG3408	GDB1	2.30e-04	264	556	202	514	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
COG4354	COG4354	4.54e-04	389	498	449	566	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].
pfam04685	DUF608	0.001	389	498	106	212	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALJ42159.1	0.0	1	729	1	729
AAO76108.1	0.0	1	729	1	729
QQA06603.1	0.0	1	729	6	734
QMW89333.1	0.0	1	729	1	729
QUT70971.1	0.0	1	729	1	729

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CIH_A	0.0	1	729	3	731	Crystalstructure of a putative alpha-rhamnosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
2OKX_A	4.84e-46	269	668	503	910	Crystalstructure of GH78 family rhamnosidase of Bacillus SP. GL1 AT 1.9 A [Bacillus sp. GL1],2OKX_B Crystal structure of GH78 family rhamnosidase of Bacillus SP. GL1 AT 1.9 A [Bacillus sp. GL1]
4XHC_A	2.05e-26	305	633	212	541	ChainA, Alpha-L-rhamnosidase [Klebsiella oxytoca],4XHC_B Chain B, Alpha-L-rhamnosidase [Klebsiella oxytoca]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KM26	4.86e-15	183	671	598	1109	Bifunctional sulfatase/alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22250 PE=1 SV=2
P9WF03	9.44e-12	122	672	281	853	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000348	0.999008	0.000156	0.000180	0.000155	0.000154

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000196_01563.