CAZyme Information: MGYG000000147_00415

Basic Information

• Species: Bacillus paralicheniformis
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus paralicheniformis
• CAZyme ID: MGYG000000147_00415
• CAZy Family: CE12
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
543	MGYG000000147_1|CGC6	60204.07	7.4415

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000147	4371304	Isolate	United Kingdom	Europe

• Gene Location: Start: 398829; End: 400460 Strand: +

Full Sequence      

MKRYVRCVWAILLLFFFSTGIEGPAQPKAAAAHHSQDLFLFDFGKGPAEKDYVKVNEASA
YQNGKGYGFSRAGNAVCKKTQKKEALKKDYCVVNRTAFLAVLSPGEYRVTVFASSRGELK
LYAEGEEMGTIPAGAKGSSAEKAFTVTVEDGRLDLEWKGPQPKVYAVEISKVYRFDFGDG
PLASGFIPIDAQSAYTPLKGYGFDDTTKVTCTDRGIADPLRSDFCIPDGTAFTVDLPDGQ
YHVRLITGDARAKTNMDVRANGMIQLYSFGAEAGGYNEKTFPIHISGGRLSIQALAARTA
PRLNALVLTKTAYHVTDDKTTVFLAGDSTVSDYEPGYAPMAGWGQFLGGYFSDEIVIDNQ
AKRARSAKSFVDERSLDTILNRMKRGDYLLIQFGINDAGQDPARHTDPYTTFQEYLRTYI
NKAREKGGRPVLVTSQSKRTYDEKGVFYNSIGEYPNAMRQLGREMNVPVLDLNKKSIDYY
NEIGVEATKNVFMFLEPGESPNYPDGIQDRVHVHEFGANQLARLVTEEIADMNMYPLKHS
LKK

Enzyme Prediction     

No EC number prediction in MGYG000000147_00415.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CE12	321	529	4.7e-74	0.9952380952380953

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01821	Rhamnogalacturan_acetylesterase_like	9.72e-99	320	529	1	198	Rhamnogalacturan_acetylesterase_like subgroup of SGNH-hydrolases. Rhamnogalacturan acetylesterase removes acetyl esters from rhamnogalacturonan substrates, and renders them susceptible to degradation by rhamnogalacturonases. Rhamnogalacturonans are highly branched regions in pectic polysaccharides, consisting of repeating -(1,2)-L-Rha-(1,4)-D-GalUA disaccharide units, with many rhamnose residues substituted by neutral oligosaccharides such as arabinans, galactans and arabinogalactans. Extracellular enzymes participating in the degradation of plant cell wall polymers, such as Rhamnogalacturonan acetylesterase, would typically be found in saprophytic and plant pathogenic fungi and bacteria.
COG2755	TesA	3.09e-17	321	533	10	212	Lysophospholipase L1 or related esterase [Amino acid transport and metabolism].
cd01838	Isoamyl_acetate_hydrolase_like	2.04e-08	322	475	2	165	Isoamyl-acetate hydrolyzing esterase-like proteins. SGNH_hydrolase subfamily similar to the Saccharomyces cerevisiae IAH1. IAH1 may be the major esterase that hydrolyses isoamyl acetate in sake mash. The SGNH-family of hydrolases is a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases
pfam13472	Lipase_GDSL_2	4.32e-08	326	474	3	149	GDSL-like Lipase/Acylhydrolase family. This family of presumed lipases and related enzymes are similar to pfam00657.
cd00229	SGNH_hydrolase	2.18e-06	322	482	1	163	SGNH_hydrolase, or GDSL_hydrolase, is a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the typical Ser-His-Asp(Glu) triad from other serine hydrolases, but may lack the carboxlic acid.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VEB18530.1	0.0	1	543	1	543
BCE06183.1	0.0	1	543	1	543
BCE14037.1	0.0	1	543	1	543
BCE12412.1	0.0	1	543	1	543
AGN35887.1	0.0	1	543	1	543

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2O14_A	8.70e-40	171	529	14	359	X-RayCrystal Structure of Protein YXIM_BACsu from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR595 [Bacillus subtilis]
3C1U_A	2.16e-12	320	501	1	198	ChainA, Rhamnogalacturonan acetylesterase [Aspergillus aculeatus]
1DEO_A	2.92e-12	320	450	1	147	RHAMNOGALACTURONANACETYLESTERASE FROM ASPERGILLUS ACULEATUS AT 1.55 A RESOLUTION WITH SO4 IN THE ACTIVE SITE [Aspergillus aculeatus],1DEX_A RHAMNOGALACTURONAN ACETYLESTERASE FROM ASPERGILLUS ACULEATUS AT 1.9 A RESOLUTION [Aspergillus aculeatus],1K7C_A Rhamnogalacturonan acetylesterase with seven N-linked carbohydrate residues distributed at two N-glycosylation sites refined at 1.12 A resolution [Aspergillus aculeatus],1PP4_A The crystal structure of rhamnogalacturonan acetylesterase in space group P3121 [Aspergillus aculeatus],1PP4_B The crystal structure of rhamnogalacturonan acetylesterase in space group P3121 [Aspergillus aculeatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31523	2.72e-64	322	541	8	223	Rhamnogalacturonan acetylesterase RhgT OS=Bacillus subtilis (strain 168) OX=224308 GN=rhgT PE=1 SV=1
O31528	8.91e-45	322	543	5	215	Probable rhamnogalacturonan acetylesterase YesY OS=Bacillus subtilis (strain 168) OX=224308 GN=yesY PE=1 SV=1
P42304	5.99e-41	171	529	29	374	Uncharacterized esterase YxiM OS=Bacillus subtilis (strain 168) OX=224308 GN=yxiM PE=1 SV=2
Q00017	2.05e-11	320	450	18	164	Rhamnogalacturonan acetylesterase OS=Aspergillus aculeatus OX=5053 GN=rha1 PE=1 SV=1
Q5BAA2	2.09e-10	321	492	19	201	Rhamnogalacturonan acetylesterase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=AN2528 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000474	0.938543	0.060282	0.000240	0.000223	0.000200

TMHMM  Annotations     

start	end
7	26