CAZyme Information: MGYG000000146_01534

Basic Information

• Species: Dorea formicigenerans
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Dorea; Dorea formicigenerans
• CAZyme ID: MGYG000000146_01534
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
568	MGYG000000146_3|CGC2	67028.6	5.0699

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000146	3357413	Isolate	United Kingdom	Europe

• Gene Location: Start: 85369; End: 87075 Strand: -

Full Sequence      

MQNKLKKIFGKIKREGLINAIQNYVDKEATLKKELKIIRDYHLINEEERKQQKEFKFECE
EKISIITPLYNTPKDFLIQLIDSVEKQTYSNWELCLADGSDLDHEYVREICMKYMEADNR
IIYKKLEENEGIVGNTNKAIQMATGTYLGLLDHDDILHESALYECMKEIQNSADFIYTDE
MKFEKKIEDSIDIVCKSGFGKDELRSHNYICHFVVFKKQLLDNMTEFYRREYEGSQDYDM
VLRLTEKAEKIVHIPKILYYWRVHAGSVAQNLSVKQYAVDAAIKAISDQLKRSGEKGTVK
CNLPYETIYRIEYEVPQTPVVSLCLWTEEKIDYQVYIKELLKKTSYRPLEIITTAETSFD
YDEEQIQIITLRKNDYKNRYEWFENARKSMTGQYEVYMNCECIPERKEWIEELLMYASRQ
DVGVVGVSIKNSEEQVVFGGGILDKEEGIHIMNEGLEWREQGYEANMRHVRNTTLLTSVC
MMIAKEKLDGAGGFQTDLGDCADIDICLKCLEKHYWNVWNCFAVMKYTGDKSIKDFWNND
DLLFERWKDRIEREDNYYHPLLKKLGKI

Enzyme Prediction     

No EC number prediction in MGYG000000146_01534.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	64	177	1.4e-22	0.6588235294117647

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04184	GT2_RfbC_Mx_like	2.96e-81	62	263	2	202	Myxococcus xanthus RfbC like proteins are required for O-antigen biosynthesis. The rfbC gene encodes a predicted protein of 1,276 amino acids, which is required for O-antigen biosynthesis in Myxococcus xanthus. It is a subfamily of Glycosyltransferase Family GT2, which includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds.
cd06433	GT_2_WfgS_like	2.51e-22	64	267	1	199	WfgS and WfeV are involved in O-antigen biosynthesis. Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.
pfam00535	Glycos_transf_2	4.59e-21	64	171	1	105	Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.
cd00761	Glyco_tranf_GTA_type	1.23e-18	65	253	1	156	Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.
PRK10018	PRK10018	4.61e-12	63	254	7	207	colanic acid biosynthesis glycosyltransferase WcaA.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRO35980.1	1.17e-169	4	565	3	553
QBF76213.1	1.17e-169	4	565	3	553
QMW89744.1	1.74e-121	37	562	56	605
BBK78193.1	1.74e-121	37	562	56	605
QUO22278.1	1.35e-117	45	560	80	617

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HEA_A	2.34e-10	61	271	5	225	CgTstructure in hexamer [Streptococcus parasanguinis FW213],5HEA_B CgT structure in hexamer [Streptococcus parasanguinis FW213],5HEA_C CgT structure in hexamer [Streptococcus parasanguinis FW213],5HEC_A CgT structure in dimer [Streptococcus parasanguinis FW213],5HEC_B CgT structure in dimer [Streptococcus parasanguinis FW213]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P55465	1.73e-56	63	562	368	888	Uncharacterized protein y4gI OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a03550 PE=4 SV=1
Q50864	8.48e-36	63	509	321	774	O-antigen biosynthesis protein RfbC OS=Myxococcus xanthus OX=34 GN=rfbC PE=4 SV=1
A0A0H2UR96	3.88e-16	61	288	3	244	Glycosyltransferase GlyG OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyG PE=1 SV=1
Q077R2	1.11e-12	61	271	2	204	UDP-Glc:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-glucosyltransferase WfaP OS=Escherichia coli OX=562 GN=wfaP PE=1 SV=1
Q58457	3.37e-12	63	263	10	213	Uncharacterized glycosyltransferase MJ1057 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1057 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000049	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000146_01534.