CAZyme Information: MGYG000000145_03266

Basic Information

• Species: Lacrimispora sp902363735
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lacrimispora; Lacrimispora sp902363735
• CAZyme ID: MGYG000000145_03266
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
835	MGYG000000145_8|CGC2	97180.44	5.4075

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000145	4646417	Isolate	United Kingdom	Europe

• Gene Location: Start: 181853; End: 184360 Strand: -

Full Sequence      

MGNIQSLNGIWQWRREGDGFGFVYKGLVPGTVISHMLEQKLIEDPYWRCNEYGVRELMGN
NYTYKRSFRVSGEDLQSDLVELVCHGIDTIASVCINDRLVAETDDMHRTYRFPVKEYLHP
GENKIEVFFHSPLDFVRKENAQNDIFYASTGCIRGNGALRKAHYMFGWDWGPQLPDMGIF
RNIALEYSSGAKLEDVRIRQNHEEAGRVMLHFEIKIKTLSDKVKTGQKWFTKVEIRDPEG
VLISEKIQDSKEVQTEEIIHNPRLWWPNGLGDQPLYKVSVFLLDHAEVTYDSCEYRIGLR
TVTVSTDENEYGNEFAITVNGVKIFAMGANYIPEDSIFTRVTKERTERLIEDCAAANFNC
LRVWGGGYYPDDYFYDKCDEKGILVWQDLMFGCNVYALNDHFEENIVEETKDNVRRLRNH
PCLALWCGNNEMEWGWGDEWARIKGHHPRYKADYIKIFEYILPKVIKSCDDVTFFWPSSP
SSGGAFDSPNAMNRGDQHYWEVWHSGKPFTEYGDFSFCSEYGFQSFPHSKTIASFTLKED
RNIFSRVMESHQKNPAANGKILNYIADYFLYPKDTDSLAYISQILQLKAIEYGVGHWRRK
RGQCMGSLYWQLNDCWPVASWASIDYYGRWKALHYGAKRFYSPLTISIGEEEEMSPRVSY
YVHNDTREQQEWRMEILLQDHDFHVLKKETAEGIIPPLSVFKAAEADFSQWTDREEIRSS
IFSAFRLYQGGELVQERTVLFVKPKHFDFKALAYDTDISETEESFCITVKSSSFCQYAEL
YLKNYDVVFSDNFFDITSRSGITVCINKNEFKNPVSVDLLRENLVIRSVADSYSR

Enzyme Prediction     

No EC number prediction in MGYG000000145_03266.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	689	5.2e-107	0.6702127659574468

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	6.63e-97	4	746	12	727	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	4.68e-13	756	831	6	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam17786	Mannosidase_ig	3.09e-11	658	746	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
pfam00703	Glyco_hydro_2	1.13e-09	191	300	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	4.55e-09	176	435	190	453	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SET87559.1	0.0	1	833	1	836
QRV20341.1	0.0	1	833	1	845
ADL05496.1	0.0	1	833	1	845
QQO08489.1	0.0	1	835	1	830
QOV18719.1	0.0	1	833	1	826

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BYE_A	2.73e-148	28	830	37	851	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	2.80e-148	28	830	37	851	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	7.10e-148	4	822	8	836	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	7.49e-148	4	822	10	838	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	7.70e-148	4	822	10	838	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q29444	2.89e-108	6	833	24	879	Beta-mannosidase OS=Bos taurus OX=9913 GN=MANBA PE=1 SV=1
Q95327	2.96e-107	6	833	24	879	Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=1 SV=1
Q5B7W2	2.68e-106	11	680	13	691	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
O00462	8.68e-103	6	833	24	879	Beta-mannosidase OS=Homo sapiens OX=9606 GN=MANBA PE=1 SV=3
A1CGA8	1.62e-101	27	651	31	663	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000067	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000145_03266.