CAZyme Information: MGYG000000137_01249

Basic Information

• Species: UBA7160 sp902363665
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UBA7160; UBA7160 sp902363665
• CAZyme ID: MGYG000000137_01249
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
836	MGYG000000137_5|CGC3	96890.6	4.4485

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000137	4141348	Isolate	United Kingdom	Europe

• Gene Location: Start: 155722; End: 158232 Strand: +

Full Sequence      

MVQQKIDSSWQMRRVGDEAFSPAVVPGSVYTDLLRNGQMEDPFFKDNELKALKLMDEDYE
YVTSFDCEEAICSSERKVLRFDGIDTIADVYLNGEMIGSPDNMHRTWEYDVTEEVKEKDN
ELRIVFHSPTKYIAEAFKVSKTHGSEDAMEGFVNIRKAHCMFGWDWGAHLPDAGLFRPVT
LLGIDEARIDSVYVTQEHADGKVTLHFAVDVDCCPEMEDPSGMWNAEAEGASENEADPQE
SGLYYTVKVTAPDGTQVSANGSPEQLVIEDPQLWWPNGYGRQDLYTVEVTLYFEGKELDC
WKRRIGLRTMTVDRSKNEWGENFAICVNGMDIFAMGADYIPEDHLLGRITPETTRSLLEK
CKWANFNAIRVWGGGYYPEDWFYDLCDELGFVVWQDFMFACAVYDLTPEFEENIRAEFID
NVKRLRHHASLGLWCGNNEMEEFVYAKNSWLTKPQEVRDYYLMYERILPEIVREYDPQTF
YWPSSPSSGGCMDEPQDPERGDVHYWDVWHGDKPFTEYRKFYFRFLSEFGFQSFPSIKTI
ETFTDDPADMNIFSYVMERHQRNGSANGKIMNYMQQTYRYPSDFRTVLYASQLLQADGIR
YGVEHFRRNRGRCMGAVYWQLNDCWPVASWSSVDYCGRLKALHYYAKRFFAPLMLSCEEQ
GMMSNAQPLNWQHSGEFEKSIRLNVANETREDAKVTVHWALRRATAEVIKSGEETITVPA
LKSVWLDKIEFPEADPYSEYISYELEKDGEIISAGSVNFSYPKYFRYEDPQLTCRVIGDE
IEVTAKAYAKSVEILNENEDLILSDNYFDMNAGTKRVKIESGDVSRLMLRSVYDIR

Enzyme Prediction     

No EC number prediction in MGYG000000137_01249.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	756	1e-106	0.7327127659574468

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	8.40e-104	1	656	10	635	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	2.20e-16	8	439	78	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam17786	Mannosidase_ig	3.05e-14	681	764	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
pfam00703	Glyco_hydro_2	1.04e-11	187	308	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10150	PRK10150	2.27e-10	75	478	82	442	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QJU13790.1	0.0	1	836	1	823
QQQ93614.1	0.0	1	836	1	823
ASU28856.1	0.0	1	836	1	823
ANU76049.1	0.0	1	836	1	823
QBE97789.1	0.0	1	836	15	837

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BYE_A	6.62e-150	6	836	8	848	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	6.80e-150	6	836	8	848	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYI_A	4.84e-149	6	836	6	846	Crystalstructure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYI_B Crystal structure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYG_A	4.98e-149	6	836	8	848	Crystalstructure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYG_B Crystal structure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	2.15e-142	6	660	11	661	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	7.94e-115	6	820	9	829	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
B0YBU9	3.03e-114	6	820	9	829	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
A1D911	1.16e-113	6	820	9	829	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
A1CGA8	8.62e-113	17	816	23	824	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
Q0CCA0	1.15e-112	3	804	6	791	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000066	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000137_01249.