CAZyme Information: MGYG000000135_02495

Basic Information

• Species: Murimonas intestini
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Murimonas; Murimonas intestini
• CAZyme ID: MGYG000000135_02495
• CAZy Family: GH2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
814	MGYG000000135_5|CGC1	94715.53	4.9099

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000135	5745007	Isolate	Canada	North America

• Gene Location: Start: 37753; End: 40197 Strand: +

Full Sequence      

MTEKCIDSGWSFRRVGEACWYPAKVPGSVYTDMMASGLMEDPFWKDNEEKALSLMEDDYE
YETHFQGGEIPGCEQLLLRFEGLDTIADVYLNDERIGSAENMHRIWEYDVTGKLRASENT
LRVVLHSPTEYIRKAFEESRTYGSEDAMDGFVHIRKAHCMFGWDWGAHLPDAGIFRPVRL
LGIRKGRIESVYITQEHKQESVALNLQVKVQAPGHEGSAGGGFSYEAEVTDPEGNTSVYS
LSPEKIVIDAPMLWWPNGYGKQYLYTVKVLLKAGGEIVDVWERRIGLRTMTVRRRKDEWG
ESFAHEVNGVTVFAMGADYIPEDHLLGRTSRERTRRLLQDCREAHFNAIRVWGGGLYPED
YFFDICDEMGLIVWQDFMFACAVYDLTPQFAANIRQEFIDNIVRIRHHACLGLWCGNNEM
EQFVKEGNWVTKPSEVRDYIIMYEHMIPELLREYDPQTFYWPASPSSGGSFDAPNDPDRG
DVHYWEVWHGNKPFTEYRKHFFRYASEFGFQSFPCRKTIETFTDDEKDYNPFSYIMEKHQ
RNYGANGKIMNYMQQTFCYPTDFDTFLYASQLLQAEAIRYGVEHFRRNRGRCMGAIYWQL
NDCWPVISWSSIDYTGRWKALHYYAKRFFAPVMISCQEEGMMSQAADMNREHFEFEKSIH
LNVANETMEPQSGTVFWELRTADSDIIKAGQEFIQLEPCSSKWLGKKCFDDIDIFSTYVS
YRFEQDGNTVSEGSVIFSYPKYFRYEDPCLTCETEGDEIIITSKAYAKSVEIQNSQEDLV
LEDNYFDMNKGQRRVRILRGKAEGVRGRSVFDIK

Enzyme Prediction     

No EC number prediction in MGYG000000135_02495.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	696	7.6e-105	0.6861702127659575

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.21e-101	9	677	18	690	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	2.47e-17	61	419	113	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam00703	Glyco_hydro_2	4.74e-13	187	288	2	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10150	PRK10150	2.05e-10	4	293	13	281	beta-D-glucuronidase; Provisional
pfam17786	Mannosidase_ig	4.30e-09	659	742	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBE97789.1	0.0	1	814	15	837
QIB54534.1	0.0	1	814	1	823
QMW79049.1	0.0	1	814	1	823
QJU13790.1	0.0	1	814	1	823
QQQ93614.1	0.0	1	814	1	823

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BYE_A	6.92e-150	1	814	3	848	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	7.11e-150	1	814	3	848	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYI_A	5.07e-149	1	814	1	846	Crystalstructure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYI_B Crystal structure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYG_A	5.21e-149	1	814	3	848	Crystalstructure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYG_B Crystal structure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	1.39e-148	6	798	11	816	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	2.01e-118	6	804	9	834	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
B0YBU9	7.72e-118	6	804	9	834	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
Q0CCA0	1.03e-117	3	639	6	661	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
A1D911	2.12e-117	6	804	9	834	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
Q5B7W2	3.95e-117	2	639	5	663	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000135_02495.