CAZyme Information: MGYG000000123_01853

Basic Information

• Species: Blautia sp001304935
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia sp001304935
• CAZyme ID: MGYG000000123_01853
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
813	MGYG000000123_5|CGC5	91668.14	5.6021

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000123	5803260	Isolate	Canada	North America

• Gene Location: Start: 164934; End: 167375 Strand: +

Full Sequence      

MNTTYYPINKNCRFHLGDCPDAWQAWYDDSDWESVSLPHDWSVTLPFSREYSSGTGYLAG
GIGWYRFRITPRESWKGRRLYLAFDGVYKNSRVWCNSYYLGNRPNGYISFTYDITEQFSF
DRENVICVCADHRDIADSRWFTGSGITRKVQLLVQEPVHVIPGSMAFETPEVSSQKAHFR
ASCEIKNDTQQDVQITAKFRLVKTESTESAIENCSSAADKMQAEVSVNMHIPAGTACRAK
ATGEVLSPALWSPDSPDLYTLETRLCPADDVSGEACCGKEYMADSRRVGIRSIRFDPDTG
FFLNDKNMLIKGVCVHHDAGCLGAAVPVPVWERRLKKLKDMGCNAIRMSHNPHMPELYDL
CDSMGFLVIDEAFDEWEGPKNKWSTGHNVYPPKHQGYYLDFPRWHKEDLTDLVRRDRCHP
SIIMWSIGNEIDYPNDPYCHSSFTTMTGNNDTDKPAEERQYNPKRPDAGRLAVLAGRLTK
IVKEIDTSRPVTLAAAFPELSSRLGFLDSLDVAGYNYKEHLYEESHRRFPDKPFIGSENG
HSLEAWEAVTKNPYISGQFLWTGIDYLGEAKGWPIHGSSAGLLTLAGFEKPGYYRRQSLW
SSKPMIHLSTARPDADRGEWTPVSDTWNYPEEEEVLVKCYTNLPQAEIWLNEKCLGTFQK
DPARDAIILTTAYVPGTLKALGKASDGTEVSHALTTTGTACKISLHCYEDTLPSATEPAF
RQIEVLMKDTWGNPVLSDTSLLHVNAENGRILGLENGDLADVTDYCAAFRRVYHGRLLIY
VNYIDEEKEMTITVSGDAEANIKTAVLTLPPLK

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	602	1.2e-111	0.6103723404255319

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.84e-63	7	571	14	507	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	1.10e-31	5	433	41	452	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	1.86e-30	64	432	68	419	beta-D-glucuronidase; Provisional
pfam02836	Glyco_hydro_2_C	1.02e-26	298	567	5	246	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam02837	Glyco_hydro_2_N	2.40e-25	13	153	9	166	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBE95340.1	0.0	1	813	1	828
QIB55323.1	0.0	1	813	1	828
QMW76814.1	0.0	1	813	1	828
QJU15852.1	0.0	1	813	1	821
ASU31149.1	0.0	1	813	1	821

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5T9A_A	4.05e-155	8	801	32	839	Crystalstructure of BuGH2Cwt [Bacteroides uniformis],5T9A_B Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_C Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_D Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9G_A Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_B Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_C Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_D Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis]
3GM8_A	1.43e-122	10	796	11	786	ChainA, Glycoside hydrolase family 2, candidate beta-glycosidase [Phocaeicola vulgatus ATCC 8482]
5T98_A	1.35e-114	8	809	30	818	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
6B6L_A	9.08e-114	14	781	16	750	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]
7RSK_A	3.75e-113	14	781	16	750	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7RSK_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	2.05e-131	8	803	47	845	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	2.67e-90	8	797	51	812	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	2.55e-44	28	661	75	678	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	2.90e-32	4	726	27	716	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P77989	6.77e-32	18	427	18	384	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999863	0.000124	0.000021	0.000000	0.000000	0.000001

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000123_01853.