CAZyme Information: MGYG000000114_00525

Basic Information

• Species: Streptococcus sp902363395
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus sp902363395
• CAZyme ID: MGYG000000114_00525
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1629	MGYG000000114_1|CGC8	182194.61	5.0886

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000114	2012808	Isolate	Canada	North America

• Gene Location: Start: 584932; End: 589821 Strand: -

Full Sequence      

MDQRFLEKSCRYSIRKLTVGTASVLLGAIFLGSHQVGADSIVGSQNESNHLEAAPAIESP
TDGTGEAKPENRYIPPISEEKGLSPDTAVESKHTATSTTSIEPNEERETMKVETPVAKQT
DYHLSYNQPAAATYDGWEKQALPVGNGEMGAKVFGLIGEERIQYNEKTLWSGGPQPDSTD
YNGGNYQDRYKILAEIRKALEAGDRQKAKQLAEENLVGPNNAQYGRYLSFGDIFMVFNNQ
KKGLENVTDYHRDLNITEAITTTSYSQDGTTFKRETFSSYPDDVTVTHLSKKGDKTLDFT
LWNSLTEDLLANGDYSWEYSNYKQGAVTTDSNGILLKGTVKDNGLQFASYLGIKTDGQVT
AQDGYLTVTGASYATLLLSAKTNYAQNPKTNYRKDIDVEATVKSIVESAKAKDYETLKHD
HIEDYQRLFNRVQLNLGGNKSSQTTKEALQTYDPTKGQELEELFFQYGRYLLISSSRDRT
DALPANLQGVWNAVDNPPWNADYHLNVNLQMNYWPAYMSNLAETAKPMVNYIDDMRYYGR
IAAKEYAGIESKEGQENGWLVHTQATPFGWTTPGWNYYWGWSPAANAWMMQNVYDYYKFT
KDETYLKEKIYPMLKETAKFWNSFLHYDKSSDRWVSSPSYSPEHGTITIGNTFDQSLVWQ
LFHDYMEAANHLNMDQDLVTEVKAKFDKLKPLHINQEGRIKEWYEEDSPQFTNEGIENHH
RHVSHLVGLFPGTLFDKDQPEYLEAARATLNHRGDGGTGWSKANKINLWARLLDGNRAHR
LLAEQLKSSTLENLWDTHAPFQIDGNFGATSGMAEMLLQSHTGYIAPLPALPDAWKDGQV
SGLVARGNFEVSMKWKEKNLETLAFLSHAGGDLVVDYPDIESSIVKINRKEIKPTLLKGN
RIQIATQKGDQITFEKLPERVTRLSAVRKDAVTAELDFNPVTDATHYVIRRESKDESNDA
SSVKEFLTNVTHFIDRSIDSLHSYSYSIRAMIGERSRQASEAASIQPVVELMDDRDPRVQ
YGPAFHNWEDADLFAGTEKYADLTVDSYSDNDATARISFNGRGIEIYGLKSSELGLAEVQ
IDGKKVGDLDFHTSGETEKSALIGRFTGLPDGEHLLTLTVKKEHLNRGNERSKISLDYFK
IYPGQGATVEKMDDRDSRIHYGSLFKDWSESGLYQTTEKYATLQNVDPSQYAEAKATIPF
TGTGIRIYGLKSSDYGKALVTIDGKEMPSLDFYSSADYEKNVLIGEYTNLSNGDHILTLT
VDPNSPDDRKMISLDSFDILKPSTVSLDTPSLAPIKAGDQTITLTLPSGEWDAIALHFPG
EKNPIFLRKTDEQHVTLSDGNRVLAIKDQQVELPIPETAHQKQTQIISAYAIRGKTITSP
VVSLLPAIVTSLGDQEPPVVTLPEYTEPIGTAGQEAAIHSLPEYVLRVLKDQASQVEVIS
GENEIGSDSHLEIQKVLDQGLFGKIYDAFNLQLKDKEGNPVSVKGSVLVRLPLSKEVKQV
YVLTPNRELQPLAFTLREGMIEFMAQELGTYAIVYQSEPEVGTLSPDSYEKVEPIVSGRK
VQNRKMLVSESTSPKKAGDSLMSVPNSKQDQASQRQLPKTNHEQRSYLLFLAGLSLVLAA
SFLGKKEQV

Enzyme Prediction     

EC	3.2.1.63

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	119	882	2.5e-262	0.9889196675900277

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	8.11e-63	124	386	1	233	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
TIGR01168	YSIRK_signal	5.55e-10	4	40	3	39	Gram-positive signal peptide, YSIRK family. Many surface proteins found in Streptococcus, Staphylococcus, and related lineages share apparently homologous signal sequences. A motif resembling [YF]SIRKxxxGxxS[VIA] appears at the start of the transmembrane domain. The GxxS motif appears perfectly conserved, suggesting a specific function and not just homology. There is a strong correlation between proteins carrying this region at the N-terminus and those carrying the Gram-positive anchor domain with the LPXTG sortase processing site at the C-terminus.
COG1554	ATH1	9.68e-09	513	635	353	485	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
pfam04650	YSIRK_signal	3.07e-07	8	32	1	25	YSIRK type signal peptide. Many surface proteins found in Streptococcus, Staphylococcus, and related lineages share apparently homologous signal sequences. A motif resembling [YF]SIRKxxxGxxS[VIA] appears at the start of the transmembrane domain. The GxxS motif appears perfectly conserved, suggesting a specific function and not just homology. There is a strong correlation between proteins carrying this region at the N-terminus and those carrying the Gram-positive anchor domain with the LPXTG sortase processing site at the C-terminus.
pfam03632	Glyco_hydro_65m	5.14e-07	513	635	35	167	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VED67666.1	0.0	1	1628	1	1628
VEE19354.1	0.0	1	1628	1	1628
SQH66892.1	0.0	1	1628	1	1617
AYF94131.1	0.0	1	1628	1	1627
AGY40130.1	0.0	1	1628	39	1665

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2EAB_A	1.53e-157	121	875	17	849	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAD_A	1.10e-156	121	875	17	849	ChainA, Alpha-fucosidase [Bifidobacterium bifidum],2EAD_B Chain B, Alpha-fucosidase [Bifidobacterium bifidum]
2EAE_A	2.07e-156	121	875	16	848	ChainA, Alpha-fucosidase [Bifidobacterium bifidum]
2RDY_A	7.34e-128	140	916	16	791	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]
4UFC_A	1.25e-127	121	903	21	771	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	2.07e-107	127	860	49	797	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	1.20e-83	134	873	31	776	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1
Q5AU81	1.65e-83	124	860	22	780	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=1 SV=1
Q2USL3	4.88e-62	131	860	23	699	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2
P0DTR5	8.01e-16	982	1262	806	1063	A type blood alpha-D-galactosamine galactosaminidase OS=Flavonifractor plautii OX=292800 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001451	0.997004	0.000927	0.000208	0.000196	0.000183

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000114_00525.