CAZyme Information: MGYG000000092_02301

Basic Information

• Species: UBA7160 sp902363135
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UBA7160; UBA7160 sp902363135
• CAZyme ID: MGYG000000092_02301
• CAZy Family: GH91
• CAZyme Description: Inulin fructotransferase [DFA-I-forming]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
451		49232.89	4.6106

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000092	3773903	Isolate	United Kingdom	Europe

• Gene Location: Start: 159150; End: 160505 Strand: +

Full Sequence      

MSSKNYYDVTKWNVGNSYEDIGEVINSIIADIKKRQTDSNMNEGGKPGAVIYIPSGDYHL
RTQVVIDISYLKIMGSGHGFVSSSIRYNLPENEWADLHEVWPGGSRILVDLSPEPRDEES
AGAAFYVERDGNPRISSVEFENFCIDGLHFVDDGSGMSNPENTYTNGKTGIYIGSAQDSF
RLTGMGIVYLEHGVTIYHADALSIHDNFIAECGNCIELRGWGQASKITDNLMGAGYKGYS
IFAENFGGLLITANNIFPRGTSSVHFRNVARSIITANRFHSFYPGMLVFEGNCSENMVSS
NHFLRDNEPWAPMLEYDNGLDDLYGILCISGNNNSVIANHISEIIHTQSIKPAGAKPVII
HIVSGKGNYISDNHIVATTEVADEQTKASDSCFSAQVDALTTTDKLVALDVTAVLVEKES
VQNTILDSGSEGQVIIDRTVNAFRATPVPGV

Enzyme Prediction     

EC	4.2.2.18	4.2.2.17	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH91	4	447	1.8e-194	0.9949367088607595

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd21111	IFTase	0.0	5	447	3	395	inulin fructotransferase. Inulin fructotransferase (IFTase; EC 4.2.2.17 and EC 4.2.2.18), a member of the glycoside hydrolase family 91, catalyzes depolymerization of beta-2,1-fructans inulin by successively removing the terminal difructosaccharide units as cyclic anhydrides via intramolecular fructosyl transfer. As a result, IFTase produces DFA-I (alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,1'-dianhydride) and DFA-III (alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride).
pfam18835	Beta_helix_2	6.06e-07	350	408	1	66	Beta helix repeat of Inulin fructotransferase. This region contains a right-handed parallel beta helix repeat unit found in Inulin fructotransferase. This Pfam entry includes sequences not found by pfam13229.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASM68477.1	0.0	1	451	1	451
SIP56492.1	1.41e-288	1	450	1	450
AJH00913.1	3.67e-272	1	450	1	450
QIZ08397.1	2.62e-264	1	450	1	446
QGQ44358.1	2.62e-264	1	450	1	446

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5ZKS_A	8.00e-220	1	450	1	444	Crystalstructure of DFA-IIIase from Arthrobacter chlorophenolicus A6 [Pseudarthrobacter chlorophenolicus A6],5ZKU_A Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZKU_B Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZKU_C Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZKU_D Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZKU_E Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZKU_F Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZKW_A Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with GF2 [Pseudarthrobacter chlorophenolicus A6],5ZKW_B Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with GF2 [Pseudarthrobacter chlorophenolicus A6],5ZKW_C Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with GF2 [Pseudarthrobacter chlorophenolicus A6],5ZKW_D Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with GF2 [Pseudarthrobacter chlorophenolicus A6],5ZKW_E Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with GF2 [Pseudarthrobacter chlorophenolicus A6],5ZKW_F Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with GF2 [Pseudarthrobacter chlorophenolicus A6]
5ZL5_A	1.87e-218	1	450	1	444	Crystalstructure of DFA-IIIase mutant C387A from Arthrobacter chlorophenolicus A6 [Pseudarthrobacter chlorophenolicus A6],5ZL5_B Crystal structure of DFA-IIIase mutant C387A from Arthrobacter chlorophenolicus A6 [Pseudarthrobacter chlorophenolicus A6],5ZL5_C Crystal structure of DFA-IIIase mutant C387A from Arthrobacter chlorophenolicus A6 [Pseudarthrobacter chlorophenolicus A6],5ZL5_D Crystal structure of DFA-IIIase mutant C387A from Arthrobacter chlorophenolicus A6 [Pseudarthrobacter chlorophenolicus A6],5ZL5_E Crystal structure of DFA-IIIase mutant C387A from Arthrobacter chlorophenolicus A6 [Pseudarthrobacter chlorophenolicus A6],5ZL5_F Crystal structure of DFA-IIIase mutant C387A from Arthrobacter chlorophenolicus A6 [Pseudarthrobacter chlorophenolicus A6],5ZLA_A Crystal structure of mutant C387A of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZLA_B Crystal structure of mutant C387A of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZLA_C Crystal structure of mutant C387A of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZLA_D Crystal structure of mutant C387A of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZLA_E Crystal structure of mutant C387A of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6],5ZLA_F Crystal structure of mutant C387A of DFA-IIIase from Arthrobacter chlorophenolicus A6 in complex with DFA-III [Pseudarthrobacter chlorophenolicus A6]
5ZKY_A	9.49e-208	1	450	1	419	Crystalstructure of DFA-IIIase from Arthrobacter chlorophenolicus A6 without its lid [Pseudarthrobacter chlorophenolicus A6],5ZL4_A Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6 wihout its lid in complex with GF2 [Pseudarthrobacter chlorophenolicus A6]
2INU_A	3.01e-111	1	377	9	370	Crystalstructure of Inulin fructotransferase in the absence of substrate [Bacillus sp. snu-7],2INU_B Crystal structure of Inulin fructotransferase in the absence of substrate [Bacillus sp. snu-7],2INU_C Crystal structure of Inulin fructotransferase in the absence of substrate [Bacillus sp. snu-7],2INV_A Crystal structure of Inulin fructotransferase in the presence of di-fructose [Bacillus sp. snu-7],2INV_B Crystal structure of Inulin fructotransferase in the presence of di-fructose [Bacillus sp. snu-7],2INV_C Crystal structure of Inulin fructotransferase in the presence of di-fructose [Bacillus sp. snu-7]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P19870	2.33e-91	7	407	6	390	Inulin fructotransferase [DFA-I-forming] OS=Arthrobacter globiformis OX=1665 PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000092_02301.