CAZyme Information: MGYG000000069_02742

Basic Information

• Species: Clostridium_A leptum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Clostridium_A; Clostridium_A leptum
• CAZyme ID: MGYG000000069_02742
• CAZy Family: GH39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1898	MGYG000000069_19|CGC1	203788.65	4.0449

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000069	3099132	Isolate	United Kingdom	Europe

• Gene Location: Start: 4245; End: 9941 Strand: -

Full Sequence      

MERCKKIARGAAAGLLSAAVLMSSMLTTSFGQMRASAAEVRNPQATLTVDLDPAVNTGDI
VHGAAGFLYGVSSEDVPTTNTIVPLKSKILVTKGALGTEHPYGDALDVAKTFLESGGEQV
QMYNSNYYGVFGVTATIEQYCDDLKNYICPAVVAWKEAWKEEHGTPEAPKDNIGARIDID
EAIVYVPINEGTPNGGNFQNAWKSFYDAIKSVDKNASLAGPNSAGYGTQFTSGQSNKSFV
QFCADNNCMPDVITWHELQTNCLNDMSWHMDDFRNIWESTDWSKYNEANGTEGIPEIPQI
CFNEYAEMDYCGVPGRLVNWIARIEDEKATGCLPFWHQANNLNDLASGANEGNGAWWLYK
WYGDMSGTTQPVSTSTNYDGLYGVSTMDEAKKLSTTLLGGFTGDITVQLNNVTATSTFAD
AEVVHVSVQETMFTGFHGTAYETPTILEGAYPVNDDGSVTVKIPDTLFENAYNVTVTQAS
GDEIVGLALRSSSGDVYEAEDAGLSGGAFASSAGTNPSYYMSNNGSGNRAVGMPSGSSMT
YTINVPADGKYKLDFNYGNGQGTERNDMNTHNPVNVKQTFALDGGEAETVTMESTLFQTM
TGTKTLYYDLTAGEHQITVTTADSGVDGNLLFHDFVRVSYAGVYGQELPAFNKVYEAELA
DVNRLLGNTDSTVSTETSLEGYSGGGYVMGLSDRAVTEGGGIRNTVVVEESGLYNITLRY
QSSQAGEASIYVGNTAITLDRVNKTVSLQAGDGWQEVTASVYLQKGINVVDLDTTVPAAL
DYMRVRALPAQDSSTTIEAEDAIPEELEGSIQVAESDGASGGKYVVGMKGAYQDADYLEF
TYNAPEAGKYQMQAFHSNEDLAGSHSYNIKVTDKYAVVDVNGTSQYPQFEGIVPVQAENL
KTYYFVDCGDHDPSTLSEGDELGSLNSVTDQLYGRDAKTGYYWGVAALNEQDTKAEPGSA
AFADPDDKGVYTKYQKALSNSAADVTDGKPKEASFRYADSQDTVGINPRCAFYKFELDPG
KYDVTVGMSNTWGNAGNPVVRLSADGIDEIVSDPYSSGSKTLSVDLTQAEPNDNGKVELS
VKGTTTGATLQMTYITITEPANDAGVDYFILPPGEEKVPVDNIRDAEGVYTGELADGFDW
LIDYRNLKNDTDRYFFINTFSDDTFREKTITLDLQKGENTIRIYNDNSWNVTYGGSTSQP
GLNYLANYTPNFDKFVITPMALNSAVELEEEYTIDVASTEYGIASANQNTVGENGEYTVS
MIPAEGKEIVNVLVNGADRTDDIVFDEASGAYQLKISGVSEDQKVQVYFSKPNTSKDSLK
NLYNEYKDLEKGTYSTASWDLFDAARSKAESVLNKDEPQQWEINNAYDELLAGINGLKDI
ENLVYFVDCGDHDPVTVTYGDDFGLYNSVTDQMYGVDDKAGYSWGVLMIDPREKETPGQG
STVVEGDKAVYTNYQKALSNGDSNFADGQDKTATLRYAHGQDTAGIDPRYVAYKFELDPG
KYDVEVGMSNTWGNAGSPIVTLSAGEVEDVVSEPYSSGSKTLSIDLTNAETNDSGRVELT
VKGTTTGATLQMTYITINKSVEAPVLDRLEVAAPTIVEYELGEELNTDGMVVKAVYSDGS
SKVLTAEQYTLSGFSSDKAGTKTVTVSYTENEITKTASFDVTVKEAPVVIPVDKTGLKEK
LDEAKAIGPDGVTEGSYEKLQNAIQEAEGVYNDPNASQDAVNQQIQKLIGAISGLKPVLS
GLEVSDPVKTEYAVGERLDTTGMTVTAQYSDGTTVTLASGQYTVSGFDSATAGEKQVTVS
YTEDGVTKTAAFTVTVKESSGSSEDPGTSEDPGSSEGPGTGSSGSESGGTESGTGSGTEN
NNNAQTGDGMTVPIILLSLLVAASAGIAVVLGKKRRLG

Enzyme Prediction     

No EC number prediction in MGYG000000069_02742.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04086	CBM35_mannanase-like	2.08e-13	653	774	1	106	Carbohydrate Binding Module 35 (CBM35); appended to several carbohydrate binding enzymes, including several glycoside hydrolase (GH) family 26 mannanase domains. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes, including periplasmic component of ABC-type sugar transport system involved in carbohydrate transport and metabolism, and several glycoside hydrolase (GH) domains, including GH26. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB35s belonging to this family are mannanase A from Clostridium thermocellum (GH26), Man26B from Paenibacillus sp. BME-14 (GH26), and the multifunctional Cel44C-Man26A from Paenibacillus polymyxa GS01 (which has two GH domains, GH44 and GH26). GH26 mainly includes mannan endo-1,4-beta-mannosidase which hydrolyzes 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans, and galactoglucomannans, but displays little activity towards other plant cell wall polysaccharides. A few proteins belonging to this family have additional CBM3 domains; these CBM3s are not found in the CBM6-CBM35-CBM36_like superfamily.
pfam07523	Big_3	7.60e-11	1747	1816	2	67	Bacterial Ig-like domain (group 3). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.
cd04083	CBM35_Lmo2446-like	1.15e-10	655	772	3	108	Carbohydrate Binding Module 35 (CBM35) domains similar to Lmo2446. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes. Some CBM35 domains belonging to this family are appended to glycoside hydrolase (GH) family domains, including glycoside hydrolase family 31 (GH31), for example the CBM35 domain of Lmo2446, an uncharacterized protein from Listeria monocytogenes EGD-e. These CBM35s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. GH31 has a wide range of hydrolytic activities such as alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, or alpha-1,4-glucan lyase, cleaving a terminal carbohydrate moiety from a substrate that may be a starch or a glycoprotein. Most characterized GH31 enzymes are alpha-glucosidases.
cd04083	CBM35_Lmo2446-like	1.61e-08	497	630	3	118	Carbohydrate Binding Module 35 (CBM35) domains similar to Lmo2446. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes. Some CBM35 domains belonging to this family are appended to glycoside hydrolase (GH) family domains, including glycoside hydrolase family 31 (GH31), for example the CBM35 domain of Lmo2446, an uncharacterized protein from Listeria monocytogenes EGD-e. These CBM35s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. GH31 has a wide range of hydrolytic activities such as alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, or alpha-1,4-glucan lyase, cleaving a terminal carbohydrate moiety from a substrate that may be a starch or a glycoprotein. Most characterized GH31 enzymes are alpha-glucosidases.
cd04081	CBM35_galactosidase-like	3.02e-08	497	624	3	115	Carbohydrate Binding Module family 35 (CBM35); appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. This family includes carbohydrate binding module family 35 (CBM35); these are non-catalytic carbohydrate binding domains that are appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. Examples of proteins which contain CBM35s belonging to this family includes the CBM35 of an exo-beta-1,3-galactanase from Phanerochaete chrysosporium 9 (Pc1,3Gal43A) which is appended to a GH43 domain, and the CBM35 domain of two bifunctional proteins with beta-L-arabinopyranosidase/alpha-D-galactopyranosidase activities from Fusarium oxysporum 12S, Foap1 and Foap2 (Fo/AP1 and Fo/AP2), that are appended to GH27 domains. CBM35s are unique in that they display conserved specificity through extensive sequence similarity but divergent function through their appended catalytic modules. They are known to bind alpha-D-galactose (Gal), mannan (Man), xylan, glucuronic acid (GlcA), a beta-polymer of mannose, and possibly glucans, forming four subfamilies based on general ligand specificities (galacto, urono, manno, and gluco configurations). Some CBM35s bind their ligands in a calcium-dependent manner. In contrast to most CBMs that are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates, while family GH27 includes alpha-galactosidases, alpha-N-acetylgalactosaminidases, and isomaltodextranases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBE97772.1	0.0	1	1816	1	1636
QKE72773.1	4.71e-103	29	884	20	823
ASS97004.1	1.30e-101	45	883	33	893
QUL57159.1	3.04e-99	45	882	347	1188
QYR21026.1	1.91e-98	45	884	33	889

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U22_A	6.46e-29	47	479	25	423	Structureof N2152 from Neocallimastix frontalis [Neocallimastix frontalis]
2WZ8_A	5.02e-06	798	882	12	94	ChainA, Cellulosome Protein Dockerin Type I [Acetivibrio thermocellus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0A401ETL2	4.09e-09	1739	1820	1373	1455	Exo-beta-1,6-galactobiohydrolase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=bl1,6Gal PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001044	0.997549	0.000362	0.000490	0.000292	0.000236

TMHMM  Annotations     

start	end
7	29
1870	1892