CAZyme Information: MGYG000000056_01511

Basic Information

• Species: Muribaculum sp002358615
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; Muribaculum; Muribaculum sp002358615
• CAZyme ID: MGYG000000056_01511
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1241	MGYG000000056_11|CGC1	140009.81	5.5554

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000056	3324937	Isolate	United Kingdom	Europe

• Gene Location: Start: 44104; End: 47829 Strand: +

Full Sequence      

MTQAIKYLLACAALIPAQSVYSGNNGDNCRSYDFSDSALPLTTVGRGSCSVTDGVLRSQG
AYALFGSPDMKNYSFSFKARAPQEAEQVQIWGGFRNSNRFDRYMVGIKGGLQDDVYLMRT
GYMGTDEFMGVRPLNFHPVPGEWYTLRVEACGNRIRVFVGDEPLPYIDVTDSNGEALTPS
GEVSLGGAWIETEFDDLVITPLNDNALDGIADTEYCQRLTPVQKENMRREQRAAYHPIKA
GRLNGSRTDISLDGNWLFMPVYECDDELSAASPAADDSNWHVMTVPDFWNPIRIWLHGET
MPSPRGQQHKGVSDTYYQRETDRCENYTFDYRKTGAAWYRQWLELPADVKGRQMTLTFDA
VSKTAEIYINGRQAATHVGMFGEIVLDATPYLHPGRNLIAVKVTRDINDTAAQTSDAMEN
YYSSVRKDVEDNKDDAQANKTVLTDIPHGFYGDNPAGIWQPVKLTISDPLKVEDVFIKPA
LDGAEFDITVKNSSLKGKRKFAVRADIVDRQSRDTLYSATLHSGLSLGAGQSATYTYRVD
GLNPKLWEPATPNLYDFTFSLVSDGGSKEIDRTTITSGFRTFEARNDGFLYLNGRRYWLR
GGNHIPFAICPNDEKLADTFMQLMREGNVNSTRTHTTPWNEMWMAAADRNGIAVSFEGTW
SWLMIHSTPIPDKGVLELWADEWLKVMNKYKNHPSLVLWTVNNEMKFYDLDADKERAMQK
FRIISDVVKRMRALDPTRPVSFDSNYIRRNGLNRFGSDFMATVDDGDIDDNHAYYNWYDF
SLFRFFNGEFQKQFKTPGRPLISQEMSTGYPNGETGHPTRSYQLIHQNPYTLIGYEAYDW
ADPASFLKVQSFITGELAEALRRTGDQVSGIMHFAYMTWFRQCYDHKAITPWPTYHALKR
ALQPVLVSAELWGRNLYAGERLPVRIYTVNDDEQGRDLPATTLEWRITDDNDRTLASGTE
LFPEVPYYGRRYIEPDIALPQTLPADKVKSKLKLRLTGKDSVLSENEYDLLLARKDWNSG
KVLTDKSTPILILDRNGMTDVLNHLGIKTTPITSASDLLKSKHKKSLCIISGGAECSDAE
AAALRAYQSAGGKLLILDSKETARKIYPEYITGWIIPTEGDISVMERPDAGVFDDIDVLE
LRYFNNNRREIPLVCNAALKAVRHQNVTELASQMKIHAYIDGGKPEDRIERIESMRGLTL
LGIRDGKGDAIVSTMCTEKATTDPIAGRLLVNLINELITTK

Enzyme Prediction     

No EC number prediction in MGYG000000056_01511.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	243	984	1.4e-88	0.7047872340425532

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	2.54e-33	335	778	65	476	beta-D-glucuronidase; Provisional
COG3250	LacZ	1.60e-31	246	909	9	636	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	4.23e-20	222	743	9	475	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02836	Glyco_hydro_2_C	4.86e-17	587	842	5	244	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam02837	Glyco_hydro_2_N	2.08e-10	251	405	3	135	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT39629.1	0.0	1	1238	1	1230
AAO76883.1	0.0	1	1238	1	1230
QMW88738.1	0.0	1	1238	1	1230
QQA09898.1	0.0	1	1238	1	1230
ALJ40807.1	0.0	1	1238	1	1230

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5UJ6_A	7.46e-25	244	809	22	522	CrystalStructure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],5UJ6_B Crystal Structure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],6NZG_A Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis],6NZG_B Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis]
3GM8_A	1.49e-24	270	741	24	422	ChainA, Glycoside hydrolase family 2, candidate beta-glycosidase [Phocaeicola vulgatus ATCC 8482]
6D50_A	2.26e-24	243	809	29	530	Bacteroidesuniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii],6D50_B Bacteroides uniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii]
6D8G_A	4.60e-23	243	809	29	530	D341AD367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii],6D8G_B D341A D367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii]
6U7I_A	1.11e-21	244	704	7	412	Faecalibacteriumprausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_B Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_C Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_D Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	2.56e-23	246	746	50	472	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KM09	1.79e-18	242	704	41	432	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
A7KGA5	2.70e-17	232	842	27	573	Beta-galactosidase OS=Klebsiella pneumoniae OX=573 GN=lacZ PE=3 SV=1
A6TI29	2.70e-17	232	842	27	573	Beta-galactosidase 2 OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) OX=272620 GN=lacZ2 PE=3 SV=1
P77989	2.44e-16	247	715	3	398	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000209	0.999192	0.000179	0.000140	0.000130	0.000122

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000056_01511.